Retroviral-mediated transmission of a mouse VL30 RNA to human melanoma cells promotes metastasis in an immunodeficient mouse model

Abstract

Infection of a human melanoma cell line by a retroviral vector resulted in transmission of a mouse VL30 (mVL30-1) retroelement RNA to some of the cells infected by the retrovirus, followed by synthesis, integration, and expression of the mVL30-1 cDNA. One vector carried a tissue factor (TF) transgene that generated high TF melanoma clones, and another vector was a control without the TF transgene that generated low TF clones. Some high TF melanoma clones contained the mVL30-1 retroelement and others did not, and some low TF melanoma clones contained the mVL30-1 retroelement and others did not. Each type of melanoma clone was tested for its metastatic potential in severe combined immunodeficient (

Binding of mouse VL30 retrotransposon RNA to PSF protein induces genes repressed by PSF: Effects on steroidogenesis and oncogenesis

“…This study expands on an earlier finding that a retroviral vector used to transfect TF cDNA into a human melanoma line also transmitted mVL30-1 RNA from the retrovirual packaging cell line (2). The transfected melanoma cells that expressed high levels of TF protein and mVL30-1 RNA together, but not separately, showed a strong increase in metastatic potential.…”

“…The proposed regulatory role for endogenous mVL30 RNA in mouse steroidogenesis could also account for the effect of exogenous mVL30 RNA in promoting metastasis of human tumor cells (2). Most of the 11 genes induced by mVL30-1 RNA in human tumor cells (Table 1) also are induced by exposing the cells to IGF1, and a high serum level of IGF1 is associated with an increased risk for developing several types of human cancer (17).…”

“…The Th and Te lines were grown as a monolayer to Ϸ70% confluence, and total RNA was isolated with TRIzol. RT-PCR was performed as described (2). The beta-actin mRNA was used to normalize the amounts of RNA in the two cell samples.…”

“…The human melanoma lines Th, T4, Te, L1, L3, L4, and L8 were cloned from the parental line YU-SIT1 by infection with a retroviral vector (2). mVL30-1 RNA was retrovirally transmitted to the Th, L3, and L8 lines but not to the T4, Te, L1, and L4 lines.…”

Long non-coding RNAs drive metastatic progression in melanoma (Review)

“…fascinatingly, this tumour-suppressor function is eradicated by the binding of a mouse retrotransposon lncrna, VL30-1, to the rna-binding domain motifs present in PSf. Moreover, retroviral transmission of VL30-1 to human melanoma cells promoted metastatic progression in immunocompromised mice, suggesting a possible role for PSf in the aetiology of metastatic melanoma (61,62). While attention-grabbing, the absence of a human homologue of the mouse VL30-1 lncrna raised doubts regarding the existence of a similar intrinsic mechanism in humans.…”