Reversal of resistance in bacteria underlies synergistic effect of essential oils with conventional antibiotics

Lahmar, Aida; Bedoui, Ahmed; Mokdad-Bzéouich, Imèn; Dhaouifi, Zaineb; Kalboussi, Zahar; Chéraif, Imed; Ghédira, Kamel; Chekir‐Ghedira, Leila

doi:10.1016/j.micpath.2016.10.018

Cited by 47 publications

(42 citation statements)

References 27 publications

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“…Our results from time-kill studies demonstrated that EbEO is an antibacterial agent showing a synergetic interaction with ampicillin, chloramphenicol, and kanamycin; and additive effects with ciprofloxacin and erythromycin. The improvement of antibiotic action has been reported for several EOs and isolated sesquiterpenes (Langeveld et al, 2014;Lahmar et al, 2017;Espinoza et al, 2019;Khoury et al, 2019).…”

Section: Discussionmentioning

confidence: 94%

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Filho

Silva

et al. 2020

Front. Microbiol.

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Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including multidrug resistance isolates) with values ranging from 8 to 516 µg/mL. EbEO also synergistically increased the action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX), an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of S. aureus infection in G. mellonella, as shown by lower levels of melanin production in those larvae. In summary, our data suggest that EbEO is a potential source of lead molecules for development of new therapeutic alternatives against S. aureus.

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Section: Discussionmentioning

confidence: 94%

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Filho

Silva

et al. 2020

Front. Microbiol.

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“…Based on literature survey, no similar results have been yet described with TTO and MRSA and they appear to be crucial when considering the possibility of combining TTO with beta-lactams for MRSA infection. In fact, the activity of other EOs was evaluated against several MRSA strains [ 41 , 42 ] and the tendency of EOs to reduce antibiotic resistance was observed, with the combination natural compounds–synthetic drugs inducing the reversal of resistance in bacteria toward antibiotics such as penicillin [ 4 ], ampicillin/sulbactam [ 5 ], carbapenems [ 43 ], and oxacillin [ 44 ]. The mode of action of antimicrobial combination leading to the synergism is still an area of active research and it is probably attributed to the perturbation of bacterial membrane or the inhibition of PBP2a activity or its reduction [ 44 ] exerted by natural compounds.…”

Section: Discussionmentioning

confidence: 99%

High Potency of Melaleuca alternifolia Essential Oil against Multi-Drug Resistant Gram-Negative Bacteria and Methicillin-Resistant Staphylococcus aureus

Oliva

Costantini

et al. 2018

Molecules

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Purpose: Herein, an extended investigation of Tea tree oil (TTO) against a number of multi-drug resistant (MDR) microorganisms in liquid and vapor phases is reported. Methods: The activity of TTO was tested against methicillin-sensitive Staphylococcus aureus (MSSA), Escherichia coli, and clinical strains of methicillin-resistant S. aureus (MRSA), extended-spectrum beta lactamases producer carbapenem-sensitive Klebsiella pneumoniae (ESBL-CS-Kp), carbapenem-resistant K. pneumoniae (CR-Kp), Acinetobacter baumannii (CR-Ab), and Pseudomonas aeruginosa (CR-Pa). Minimal inhibitory/bactericidal concentrations (MIC/MBCs) and synergistic activity between TTO and different antimicrobials were determined. In the vapor assay (VP), TTO-impregnated discs were placed on the lid of a petri dish and incubated for 24 h at 37 °C. Results: TTO showed a potent bactericidal activity against all the tested microorganisms. TTO in combination with each reference antimicrobial showed a high level of synergism at sub-inhibitory concentrations, particularly with oxacillin (OXA) against MRSA. The VP assay showed high activity of TTO against CR-Ab. Conclusion: Evaluation of in-vitro activity clearly indicated TTO as a potential effective antimicrobial treatment either alone or in association with known drugs against MDR. Therefore, TTO could represent the basis for a possible role in non-conventional regimens against S. aureus and Gram-negative MDR. TTO in VP might represent a promising option for local therapy of pneumonia caused by CR-Ab.

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“…Similarly, Mun and colleagues also showed that a plant-derived flavonol, morin reversed the oxacillin-and ampicillin-treated MRSA [124]. Essential oils derived from Pituranthos chloranthus, Teucrium ramosissimum and Pistacia lentiscus also reduced the resistance of MRSA to various antibiotics in Penicillins' group such as amoxicillin, piperacillin, and oxacillin [125].…”

Section: Synergism Of Natural Products and Antibioticsmentioning

confidence: 87%

Non-microbial Natural Products That Inhibit Drug-Resistant Staphylococcus aureus

Chew¹,

Peh²,

Yeang³

2019

Staphylococcus Aureus

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Drug resistance developed in human pathogenic bacteria is emerging and has become a global problem. Methicillin-resistant Staphylococcus aureus (MRSA) spreading in both hospital and community areas has posed a great impact to global public health. Current antibiotics used against these resistant strains are no longer efficacious and the search for new alternative is in urgent need. In the past decades, natural products have demonstrated multiple biological activities in biomedical areas including their antibacterial actions against various drug-resistant bacteria. More promisingly, some natural products could reverse the resistance of bacteria to the antibiotics, making the target bacteria susceptible to these drugs again. Numerous natural products have also exhibited potent synergism against the drug-resistant bacteria when used in combination with various types of antibiotics. Recently, several antibacterials derived from microbes have been developed and approved by Food and Drug Administration (FDA) for clinical use. In this chapter, we discuss the potential use of non-microbial natural products in controlling Staphylococcus aureus (S. aureus)'s growth, and the underlying challenges in developing the natural products into clinical applications.

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Reversal of resistance in bacteria underlies synergistic effect of essential oils with conventional antibiotics

Cited by 47 publications

References 27 publications

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

High Potency of Melaleuca alternifolia Essential Oil against Multi-Drug Resistant Gram-Negative Bacteria and Methicillin-Resistant Staphylococcus aureus

Non-microbial Natural Products That Inhibit Drug-Resistant Staphylococcus aureus

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