Reverse Transcriptase and Reverse Splicing Activities Encoded by the Mobile Group II Intron COBI1 of Fission Yeast Mitochondrial DNA

Schäfer, Bernd; Gan, Lin; Perlman, Philip S.

doi:10.1016/s0022-2836(03)00441-8

Cited by 19 publications

(12 citation statements)

References 42 publications

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“…The blots were hybridized with various 5′-end-labeled 50-mer oligonucleotides, as described in Ref. 37. Hybridization signals were measured using a Fuji LAS-1000 imager.…”

Section: Northern Blot Analysis In S Pombementioning

confidence: 99%

The 3′ Ends of Mature Transcripts Are Generated by a Processosome Complex in Fission Yeast Mitochondria

Hoffmann¹,

Nickel²,

Speer³

et al. 2008

Journal of Molecular Biology

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“…The blots were hybridized with various 5′-end-labeled 50-mer oligonucleotides, as described in Ref. 37. Hybridization signals were measured using a Fuji LAS-1000 imager.…”

Section: Northern Blot Analysis In S Pombementioning

confidence: 99%

The 3′ Ends of Mature Transcripts Are Generated by a Processosome Complex in Fission Yeast Mitochondria

Hoffmann¹,

Nickel²,

Speer³

et al. 2008

Journal of Molecular Biology

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“…The capture of these tRNAs and/or tRNA-like structures by these plasmids has been explained by the integration of a tRNA sequence in the plasmid sequence during a reverse transcription step of a plasmid mRNA. Indeed, a reverse transcriptase activity was evidenced in fungal and plant mitochondria encoded by some group II introns (Schäfer et al, 2003). The tRNA integration would be due to the use of a tRNA for priming the cDNA synthesis (Chiang and Lambowitz, 1997) or to a template switching occurring during this synthesis as suggested for two hybrid plasmids of N. intermedia (Maas et al, 2007a).…”

Section: Dcarota Intmentioning

confidence: 99%

The Agrocybe aegerita mitochondrial genome contains two inverted repeats of the nad4 gene arisen by duplication on both sides of a linear plasmid integration site

Férandon¹,

El-Kirat-Chatel²,

Castandet³

et al. 2008

Fungal Genetics and Biology

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“…In a process referred to as retrohoming, the intron and its ORF colonize typically intron-less cognate alleles through a site-specific DNA integration mechanism known as target DNA-primed reverse transcription (Zimmerly et al 1995a,b). Retrohoming involves both the intron RNA and the IEP and is completed by host DNA repair mechanisms (Schäfer et al 2003;Lambowitz and Zimmerly 2004).…”

Section: Introductionmentioning

confidence: 99%

A group II intron encodes a functional LAGLIDADG homing endonuclease and self-splices under moderate temperature and ionic conditions

Mullineux¹,

Costa²,

Bassi³

et al. 2010

RNA

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A group II intron encoding a protein belonging to the LAGLIDADG family of homing endonucleases was identified in the mitochondrial rns gene of the filamentous fungus Leptographium truncatum, and the catalytic activities of both the intron and its encoded protein were characterized. A model of the RNA secondary structure indicates that the intron is a member of the IIB1 subclass and the open reading frame is inserted in ribozyme domain III. In vitro assays carried out with two versions of the intron, one in which the open reading frame was removed and the other in which it was present, demonstrate that both versions of the intron readily self-splice at 37°C and at a concentration of MgCl 2 as low as 6 mM. The open reading frame encodes a functional LAGLIDADG homing endonuclease that cleaves 2 (top strand) and 6 (bottom strand) nucleotides (nt) upstream of the intron insertion site, generating 4 nt 39 OH overhangs. In vitro splicing assays carried out in the absence and presence of the intron-encoded protein indicate that the protein does not enhance intron splicing, and RNA-binding assays show that the protein does not appear to bind to the intron RNA precursor transcript. These findings raise intriguing questions concerning the functional and evolutionary relationships of the two components of this unique composite element.

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Reverse Transcriptase and Reverse Splicing Activities Encoded by the Mobile Group II Intron COBI1 of Fission Yeast Mitochondrial DNA

Cited by 19 publications

References 42 publications

The 3′ Ends of Mature Transcripts Are Generated by a Processosome Complex in Fission Yeast Mitochondria

The 3′ Ends of Mature Transcripts Are Generated by a Processosome Complex in Fission Yeast Mitochondria

The Agrocybe aegerita mitochondrial genome contains two inverted repeats of the nad4 gene arisen by duplication on both sides of a linear plasmid integration site

A group II intron encodes a functional LAGLIDADG homing endonuclease and self-splices under moderate temperature and ionic conditions

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