Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

Ivanović, Darko; Popović, Ana; Radulovic, D.; Medenica, Mirjana

doi:10.1016/s0731-7085(98)00109-5

Cited by 87 publications

(34 citation statements)

References 6 publications

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“…HPLC was used to determine the content of vitamins C (Ivanovic et al 1999). The level of this vitamin was assayed after protein precipitation by mixing 300 ll of the liver homogenate with an equal volume of 100 g/L metaphosphoric acid.…”

Section: Biochemical Assaysmentioning

confidence: 99%

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

2005

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The aim of this paper is to assess the antioxidant properties of rat liver in the course of acute and chronic fasciolosis. Wistar rats were infected per os with 30 metacercariae of Fasciola hepatica. Liver activities of antioxidant enzymes and concentrations of non-enzymatic antioxidants were determined at 4, 7, and 10 weeks post-infection. Activities of superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GSH-Px), and glutathione reductase (GSSG-R) were decreased, catalase (CAT) activity was increased and non-enzymatic antioxidant concentrations (reduced glutathione, vitamins C, E and A) were reduced simultaneously with enhancement of lipid peroxidation processes as evidenced by increased levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). Changes in the antioxidant abilities of the liver and in the phospholipid structure of the cell membrane were accompanied by rising activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as markers of liver damage.

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Section: Biochemical Assaysmentioning

confidence: 99%

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

2005

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“…Many analytical methods, such as those based on UV-Vis spectrophotometry, 8 fluorimetry, 9 chemiluminiscence, 10 capillary electrophoresis, 11 microbiology, 12 and high-performance liquid chromatography [13][14][15][16][17] have been proposed only for the determination of water-soluble or fat-soluble vitamins and are tedious, sometimes nonspecific, and time-consuming. 18 Among them, both normal and reversed-phase HPLC 19 techniques are most widely used and provide rapid, sensitive, and accurate vitamin analysis and are often used with detection modes, including UV spectrometry with a diode array, fluorometry, and electrochemical detection. [20][21][22] However, few methods using HPLC have been developed for the simultaneous determination of water-and fat-soluble vitamins.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Küçükkolbaşı¹,

Bilber²,

Ayyıldız³

et al. 2013

Quím. Nova

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Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

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“…Four vitamins have been determined by ATR-FTIR [14], by ion pairing in pharmaceuticals in ca. 27 min [15], and in multivitamin and milk powder, pork muscle, and haricot vert beans [16]. Six vitamins including B 6 in three forms are separated by ion-pairing HPLC in 55 min and the method was applied to infant milk [2].…”

Section: Introductionmentioning

confidence: 99%

Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction

Chatzimichalakis

Samanidou

Verpoorte

et al. 2004

J of Separation Science

109

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An HPLC method was developed for the simultaneous determination of seven water-soluble vitamins, viz. thiamine, riboflavin, nicotinic acid, nicotinamide, pyridoxine, cyanocobalamin, and folic acid, in multivitamin pharmaceutical formulations and biological fluids (blood serum and urine). Separation was achieved at ambient temperature on a Phenomenex Luna C18 (150 x 4.6 mm) analytical column. Gradient elution was performed starting at a 99:1 A:B v/v composition, where A: 0.05 M CH3COONH4/CH3OH (99/1) and B: H2O/CH3OH (50/50), at a flow rate of 0.8 mL/min. After a 4-min isocratic elution the composition was changed to 100% of B in 18 min and elution continued isocratically for 8 min. Detection was performed with a photodiode array detector at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples. Detection limits were in the range of 1.6-3.4 ng, per 20-microL injection, while linearity held up to 25 ng/microL. Accuracy, intra-day repeatability (n = 6), and inter-day precision (n = 7) were found to be satisfactory. Theobromine (2 ng/microL) was used as internal standard. Sample preparation of biological fluids was performed by SPE on Supelclean LC-18 cartridges with methanol-water 85/15 v/v as eluent. Extraction recoveries from biological matrices ranged from 84.6% to 103.0%.

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Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals

Cited by 87 publications

References 6 publications

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

Antioxidant potential of rat liver in experimental infection with Fasciola hepatica

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction

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