Superparamagnetic iron oxide nanoparticles (SPIONs) were modified with [3-(2,3-epoxy-propoxy)propyl] trimethoxy silane, which resulted in formation of epoxy groups on the particles surface. The epoxy functionalized SPIONs can bind to human serum albumin (HSA). The binding amount of HSA to epoxy modified SPIONs was found to be as 32.7 microM for 1 mg epoxy modified SPIONs at 280 and 342 nm of excitation and emission wavelengths by using fluorescence spectroscopy. Interaction of ketoconazole with HSA immobilized epoxy functionalized SPIONs was studied at 300 and 390 nm of excitation and emission wavelengths, respectively. Binding mechanism of ketoconazole and HSA was identified by Stern-Volmer equation. Thermodynamic parameters (deltaH, deltaS and deltaG) were also estimated for the interaction. Therefore, the nature of the binding forces was found to be as hydrophobic interaction. Protein and drug attachments were also examined by Infrared spectroscopy (IR) and Scanning Electron Microscopy (SEM). This study show that prepared albumin-based magnetic nanoparticles carrier systems represents an attractive strategy for drug delivery.
Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.
The purpose of present study is to investigate the effi ciency of different kinds of Turkish commercial bleaching earth materials for changes in different colour pigment concentrations in neutralized sunfl ower oils. The bleaching experiments were performed in a pilot system under at stable vacuum (50 mmHg) and temperature (100ºC) for 30 min. By examining the changes in chlorophyll, β-carotene and red colour, bleaching process parameters such as type and dosage of the bleaching material were optimised. The sorption characteristics of colour pigments were evaluated using common adsorption isotherms and Scatchard plot analysis. Ads-3 acid-activated earth material at 1% (w/w) per samples was found to be the most appropriate sorbent and the amount of sorbed pigments was calculated as 1.01x10 -4 mmol/g ads. for chlorophyll, 1.15x10 -3 mmol/g ads. for carotene and 1.70 red on Lovibond colour scale. The procedure indicated that this system can be easily adapted to the actual oil refi ning systems. Unauthenticated Download Date | 5/11/18 5:46 AM
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