Reversed-phase liquid chromatographic separation of complex samples by optimizing temperature and gradient time

Dolan, John W.; Snyder, Lloyd R.; Wolcott, Robert G.; Haber, Piotr; Bączek, Tomasz; Kaliszan, Roman; Sander, Lane C.

doi:10.1016/s0021-9673(99)00766-9

Cited by 95 publications

(40 citation statements)

References 23 publications

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“…Percentage error between experimental and calculated from Eqs. (11) and (12) The oven temperature was estimated from Eq. (7) using data of Table 1.…”

Section: Discussionmentioning

confidence: 99%

Combined effect of temperature and organic modifier concentration on the retention under single mode gradient conditions in reversed‐phase HPLC

Pappa-Louisi¹,

Nikitas²,

Zisi³

et al. 2008

J of Separation Science

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The combined effect of temperature, T, and organic modifier concentration, phi, on the retention under gradient conditions in RPLC is studied by considering, both theoretically and experimentally gradients, of phi at constant T and gradients of T at constant phi. Two approaches are examined: in the first approach the prediction of the elution time of a sample solute is based on the isocratic/isothermal properties of this solute. The second approach is based on a direct fitting procedure of a proper retention model to 2-D isocratic/T-gradient or isothermal/phi-gradient retention data. These approaches were tested using alkylbenzes in eluting systems modified by ACN. We found that both approaches can give excellent predictions under certain prerequisites. However, the first approach exhibits the notable advantage that it can be used effectively to predict retention times under any kind of phi-gradients at constant T or T-gradients at constant phi. The second approach has the advantage that it is relatively simple but its applicability is very restricted since its predictions are satisfactory only if the gradients are of the same kind with those used in the fitting procedure and the conditions lie within those used for fitting.

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“…Percentage error between experimental and calculated from Eqs. (11) and (12) The oven temperature was estimated from Eq. (7) using data of Table 1.…”

Section: Discussionmentioning

confidence: 99%

Combined effect of temperature and organic modifier concentration on the retention under single mode gradient conditions in reversed‐phase HPLC

Pappa-Louisi¹,

Nikitas²,

Zisi³

et al. 2008

J of Separation Science

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“…The accuracy of the simulation was described using two parameters [35]. One is the error du in the volume-fraction of the strong solvent u for the predicted separation, as calculated from Eq.…”

Section: Binary Mobile Phase Screening and Computation Of Isoeluotropmentioning

confidence: 99%

Wheat digalactosyldiacylglycerol molecular species profiling using porous graphitic carbon stationary phase

Deschamps

Gaudin

Baillet

et al. 2004

J of Separation Science

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The potential of porous graphitic carbon stationary phase (PGC) was assessed for the separation of molecular species of digalactosyldiacylglycerol (DGDG). Detection was by an evaporative light scattering detector (ELSD). A conventional optimization strategy allowed definition of a quaternary non-aqueous mobile phase and separation of 9 wheat DGDG molecular species with isocratic elution: methanol/toluene/tetrahydrofuran/chloroform 64.3/21.5/13.7/0.5 v/v with 0.1% of triethylamine and a stoichiometric amount of formic acid. The molecular species were identified by LC/MS. The chromatographic behavior of DGDG on PGC was then compared to previous studies. The addition of a carbon double bond on the alkyl chain decreased the retention. This contribution was less important when the number of unsaturations increased in the alkyl chain. The consequence of this retention behavior with PGC was an elution order of molecular species which did not agree with the partition number as observed with C18 grafted stationary phases.

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“…The gradient run induced a peak focusing effect coinciding with lower retention time, resulting in sharper peak shapes and less tailing. To measure this effect quantifiably, the average peak capacity was measured of the isothermal runs and the gradient run as described in literature [45][46][47]. A peak capacity of 55 was obtained for the gradient run (time interval of 20 min), while the peak capacities of the isothermal runs at 25, 40 and 60°C were 50, 54 and 38, respectively.…”

Section: Resultsmentioning

confidence: 99%

Investigation of poly(styrene-divinylbenzene-vinylsulfonic acid) as retentive and electroosmotic flow generating phase in open-tubular electrochromatography

Smet

Lynen

2015

Journal of Chromatography A

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Reversed-phase liquid chromatographic separation of complex samples by optimizing temperature and gradient time

Cited by 95 publications

References 23 publications

Combined effect of temperature and organic modifier concentration on the retention under single mode gradient conditions in reversed‐phase HPLC

Combined effect of temperature and organic modifier concentration on the retention under single mode gradient conditions in reversed‐phase HPLC

Wheat digalactosyldiacylglycerol molecular species profiling using porous graphitic carbon stationary phase

Investigation of poly(styrene-divinylbenzene-vinylsulfonic acid) as retentive and electroosmotic flow generating phase in open-tubular electrochromatography

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