Reversed-phase liquid chromatography on an amide stationary phase for the determination of the B group vitamins in baby foods

Viñas, Pilar; López-Erroz, Carmen; Balsalobre, Nuria; Hernández‐Córdoba, Manuel

doi:10.1016/s0021-9673(03)00977-4

Cited by 89 publications

(47 citation statements)

References 26 publications

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“…Several stationary phases have been used to separate water-soluble vitamins, like C 18 -reverse phase, reverse phase with ion-pairing reagent, normal phase, ion exchange phase and special bonded phase. 33 Generally, C 18 -reverse phase has been considered the gold standard in vitamin analysis, due to its simplicity and ruggedness. If there are very different polarities of vitamins, however, a more suitable column must be used.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS C 30 (4.6 mm × 250 mm, 5 µm particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients (r 2 ) higher than 0.995. The developed method was validated with respect to linearity, intra-and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra-and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…The extraction method consisted of the combination of acid and enzymatic digestion of food samples. The procedure was based on the method described by Viñas et al (28), but several improvements were introduced. Food samples of 0.5 g Products from different producers.…”

Section: Methodsmentioning

confidence: 99%

Gluten-Free Flours from Different Raw Materials as the Source of Vitamin B1, B2, B3 and B6

Rybicka

Gliszczyńska‐Świgło

2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary Gluten-free (GF) products are those with a natural absence or acceptable level (,20 mg/kg) of gluten. They should be a part of a diet for people with gluten-related disorders, like celiac disease. Recently the popularity of a gluten-free diet (GFD) has risen extremely, because a lot of healthy individuals exclude gluten from their menus. According to the literature data on nutritional deficiencies in the GFD, this trend seems to be risky. This paper describes the nutritional value of 14 flours from different GF raw materials from the aspect of B-group vitamin content (B1, B2, B3, B6). Vitamins were determined using high performance liquid chromatography after enzymatic and acid hydrolysis of the samples. The vitamin contents significantly differed in the analysed flours. The content (in 100 g of the product) of vitamin B1 ranged from 0.01 mg (amaranth flour) to 0.60 mg (teff flour), vitamin B2 from 0.03 mg (GF flour with oats) to 0.22 mg (buckwheat flour), vitamin B3 from below 0.01 mg (amaranth flour) to 6.02 mg (millet flour), and vitamin B6 from 0.03 mg (acorn flour) to 0.69 mg (amaranth flour). The content of vitamins in the analysed GF flours was also compared to gluten-containing flours. Obtained results indicate that flours from teff, millet, chestnut, buckwheat, and amaranth are better sources of certain B-group vitamins than flours from corn, rice, and some flours with gluten.

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“…26 Therefore, the DAD and FLD detectors were tested at several wavelengths by using 10 mg L −1 standards of the water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, p-aminobenzoic acid) and the fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, phytonadione). The DAD detector was found to be more appropriate for some of the vitamins, whereas the FLD detector was better suited for others.…”

Section: Selection Of Appropriate Detector and Wavelengthmentioning

confidence: 99%

“…4,5 The standard 6 and official analytical methods for vitamin analysis 7 are tedious and involve a pre-treatment of the sample through complex chemical, physical, or biological reactions in order to eliminate interference of other constituents in the vitamin, which is typically followed by individual methods for each different vitamin. Many analytical methods, such as those based on UV-Vis spectrophotometry, 8 fluorimetry, 9 chemiluminiscence, 10 capillary electrophoresis, 11 microbiology, 12 and high-performance liquid chromatography [13][14][15][16][17] have been proposed only for the determination of water-soluble or fat-soluble vitamins and are tedious, sometimes nonspecific, and time-consuming. 18 Among them, both normal and reversed-phase HPLC 19 techniques are most widely used and provide rapid, sensitive, and accurate vitamin analysis and are often used with detection modes, including UV spectrometry with a diode array, fluorometry, and electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

Küçükkolbaşı¹,

Bilber²,

Ayyıldız³

et al. 2013

Quím. Nova

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Recebido em 14/10/12; aceito em 22/2/13; publicado na web em 24/5/13In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.

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Reversed-phase liquid chromatography on an amide stationary phase for the determination of the B group vitamins in baby foods

Cited by 89 publications

References 26 publications

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Gluten-Free Flours from Different Raw Materials as the Source of Vitamin B<sub>1</sub>, B<sub>2</sub>, B<sub>3</sub> and B<sub>6</sub>

Simultaneous and accurate determination of water- and fat-soluble vitamins in multivitamin tablets by using an RP-HPLC method

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