Reversible electrochemical monitoring of surface confined reactions at liquid–liquid interfaces by modulation of ion transfer fluxes

Xu, Yufeng; Shvarev, Alexey; Bakker, Eric

doi:10.1039/b503548a

Cited by 16 publications

(25 citation statements)

References 16 publications

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“…Here, however, this process is instrumentally controlled and leads to reversible sensor signals (see also Voltammetry: ITIES). 7 Non-equilibrium potentiometry is a promising technique that widens possible applications of ion-selective electrodes. Historically, however, steady-state concentration gradients across ion-selective membranes have been too slow to establish across such membranes and resulted in potential drifts.…”

Section: Ises: New Mechanisms Unusual Analytesmentioning

confidence: 99%

Electrochemical Sensors

2006

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Section: Ises: New Mechanisms Unusual Analytesmentioning

confidence: 99%

Electrochemical Sensors

2006

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“…In this paper [10], EIS showed that solution adsorption of commercially available non-ionic surfactants can be used to reproducibly produce a SAM of the surfactant at the electrode/electrolyte interface and, the electrochemically imposed ion flux at the interface of a chronopotentiometric polymer ISE, is modulated by the SAM, thereby providing a promising experimental tool for the detection of surface confined reactions (e.g. a biorecognition event).…”

Section: Introductionmentioning

confidence: 98%

“…A natural extension of the work by Muslinkina and Pretsch [8,9] was to study the solution adsorption of surfactants onto the polymeric ISE membrane, and this research was undertaken by Xu et al [10]. In this paper [10], EIS showed that solution adsorption of commercially available non-ionic surfactants can be used to reproducibly produce a SAM of the surfactant at the electrode/electrolyte interface and, the electrochemically imposed ion flux at the interface of a chronopotentiometric polymer ISE, is modulated by the SAM, thereby providing a promising experimental tool for the detection of surface confined reactions (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, this fundamental study has been undertaken to ascertain if a blocked interface polymeric membrane ISE is a suitable biosensor technology platform when used in conjunction with chronopotentiometric polymeric membrane ISEs. Given that EIS and atomic force microscopy (AFM) have been applied successfully in previous studies of the influence of solution adsorption of surfactants [10] and polyelectrolytes [11] on the kinetics of ion transfer with these blocked interface polymeric membrane ISEs, the use of EIS and AFM in a study of the surface blocking of polymeric membrane ISEs by an avidin-biotin biorecognition element was deemed appropriate.…”

Section: Introductionmentioning

confidence: 99%

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Detecting Biorecognition Events at Blocked Interface Polymeric Membrane Ion‐Selective Electrodes Using Electrochemical Impedance Spectroscopy and Atomic Force Microscopy

Panduwinata

2008

Electroanalysis

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Immobilization of a biorecognition element onto a polymeric membrane ion-selective electrode (ISE) using a selfassembly approach may provide scope for a novel biosensor technology platform based on the altered potentiometric response at the blocked ISE interface. In this paper, the authors have investigated the influence of solution adsorption of the model biorecognition element, avidin-biotin, on the electrode kinetics of a conventional polymeric membrane Ca 2þ ISE using atomic force microscopy (AFM) coupled with electrochemical impedance spectroscopy (EIS). It is demonstrated that solution adsorption of avidin followed by biotin incorporation leads to a demonstrable biorecognition event characterized by an impediment in the Ca 2þ ion transfer kinetics of the modified ISE surface. This kinetic principle is amenable to biosensing using pulsed chronopotentiometric polymeric ISEs, which is an established dynamic electrode technique for use with polymeric membrane ISEs.

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“…In this case, the binding event is expected to increase the energetics of the instrumentally imposed ion flux across the chemically modified sample–membrane interface. Early work with this general protocol has been performed on non-specific membrane coatings, including surfactants and polyelectrolyte layers 17, 18. It was established that the technique is most sensitive to surface blocking events under conditions where a depletion of the marker ion in the aqueous phase boundary of the membrane is observed 17, 19.…”

Section: Introductionmentioning

confidence: 99%

Ion Channel Mimetic Chronopotentiometric Polymeric Membrane Ion Sensor for Surface-Confined Protein Detection

Xu¹,

Bakker

2008

Langmuir

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The operation of ion channel sensors is mimicked with functionalized polymeric membrane electrodes, using a surface confined affinity reaction to impede the electrochemically imposed ion transfer kinetics of a marker ion. A membrane surface biotinylated by covalent attachment to the polymeric backbone is used here to bind to the protein avidin as a model system. The results indicate that the protein accumulates on the ion-selective membrane surface, partially blocking the current induced ion transfer across the membrane/aqueous sample interface, and subsequently decreases the potential jump in the so-called super-Nernstian step that is characteristic of a surface depletion of the marker ion. The findings suggest that such a potential drop could be utilized to measure the concentration of protein in the sample. Because the sensitivity of protein sensing is dependent on the effective blocking of the active surface area, it can be improved with a hydrophilic nanopore membrane applied on top of the biotinylated ion-selective membrane surface. Based on cyclic voltammetry characterization, the nanoporous membrane electrodes can indeed be understood as a recessed nanoelectrode array. The results show that the measuring range for protein sensing on nanopore electrodes is shifted to lower concentrations by more than one order of magnitude, which is explained with the reduction of surface area by the nanopore membrane and the related more effective hemispherical diffusion pattern.

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Reversible electrochemical monitoring of surface confined reactions at liquid–liquid interfaces by modulation of ion transfer fluxes

Cited by 16 publications

References 16 publications

Electrochemical Sensors

Electrochemical Sensors

Detecting Biorecognition Events at Blocked Interface Polymeric Membrane Ion‐Selective Electrodes Using Electrochemical Impedance Spectroscopy and Atomic Force Microscopy

Ion Channel Mimetic Chronopotentiometric Polymeric Membrane Ion Sensor for Surface-Confined Protein Detection

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