Reversible gelation of cells using self-assembling hydrophobically-modified biopolymers: towards self-assembly of tissue

Javvaji, Vishal; Dowling, Matthew B.; Oh, Hyun‐Taek; White, Ian M.; Raghavan, Srinivasa R.

doi:10.1039/c4bm00017j

Cited by 26 publications

(56 citation statements)

References 25 publications

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“…In contrast, a solution of 1 wt% native alginate was mixed at the same volume ratio with blood, and the resulting mixture remained as a freely flowing 6 viscous liquid (Figure 1, right). This result not only corroborated earlier in vitro studies, 14 but also gave us an initial indication that the hydrophobic modification chemistry was successful.…”

Section: Resultssupporting

confidence: 87%

“…Previous work has shown that hm-alginate, much like hm-chitosan, forms a 3D network and gels when mixed with cells. 14 In this study, we will test the hypothesis that hm-alginate, prepared as a compression dressing in sponge format, will have similar increase in hemostatic effect as previously illustrated with hm-chitosan in vivo. While this work focuses on topical treatment of hemorrhage in traumatic wounds, future work with hm-alginate will focus on internal surgical applications.…”

Section: Introductionmentioning

confidence: 95%

“…Hmalginate was synthesized as described by Javvaji et al 14 The swine were prepared, anesthetized, intubated, placed on mechanical ventilation, and maintained as described in DeCastro et al 10 Surgical procedures, fluid resuscitation, and data collection were performed as previously described, 10 except that 6.0 mm diameter vascular punch was used instead of a 4.4mm. Animal survival was defined as MAP and end tidal pCO 2 greater than 20 mmHg and 15 mmHg, respectively, after 180 minutes.…”

Section: Hm-alginate Synthesis and Preparation Of Alginate And Hm-algmentioning

confidence: 99%

See 2 more Smart Citations

Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine

Dowling¹,

Chaturvedi²,

MacIntire³

et al. 2016

Injury

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Hm-alginate demonstrates a greatly superior efficacy, relative to unmodified alginate and Kerlix™ gauze dressings, in achieving hemostasis from a lethal femoral artery puncture in swine. This is a similar result as has been previously described when performing hydrophobic modification to chitosan. The current study further suggests that hydrophobic modification of a hydrophilic biopolymer backbone can significantly increase the hemostatic capabilities relative to the native biopolymer.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 95%

Section: Hm-alginate Synthesis and Preparation Of Alginate And Hm-algmentioning

confidence: 99%

See 1 more Smart Citation

Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine

Dowling¹,

Chaturvedi²,

MacIntire³

et al. 2016

Injury

Self Cite

View full text Add to dashboard Cite

show abstract

“…6D for PEGHDI- DTH gel), both the moduli of cell-laden gels increased which is likely due to the interaction between the PU matrix through hydrophobic domains. A similar increase in dynamic moduli has been observed between cells and hydrophobically modified alginate gels which is attributed to the interactions of cells with hydrophobic domains [60]. Furthermore, G″ values show fluctuations from the intermediate frequency range which are likely due to reduced relaxation and reorganization of PEG chains in the presence of cells, similar to responses in worm-like micelles and actin-based gels which cannot recombine to form original structures at high frequencies [61,62].…”

Section: Resultsmentioning

confidence: 55%

Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells

Nalluri

Krishnan

Cheah

et al. 2015

Materials Science and Engineering: C

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Segmental polyurethanes exhibit biphasic morphology and can control cell fate by providing distinct matrix guided signals to increase the chondrogenic potential of mesenchymal stem cells (MSCs). Polyethylene glycol (PEG) based hydrophilic polyurethanes can deliver differential signals to MSCs through their matrix phases where hard segments are cell-interactive domains and PEG based soft segments are minimally interactive with cells. These coordinated communications can modulate cell–matrix interactions to control cell shape and size for chondrogenesis. Biphasic character and hydrophilicity of polyurethanes with gel like architecture provide a synthetic matrix conducive for chondrogenesis of MSCs, as evidenced by deposition of cartilage-associated extracellular matrix. Compared to monophasic hydrogels, presence of cell interactive domains in hydrophilic polyurethanes gels can balance cell–cell and cell–matrix interactions. These results demonstrate the correlation between lineage commitment and the changes in cell shape, cell–matrix interaction, and cell–cell adhesion during chondrogenic differentiation which is regulated by polyurethane phase morphology, and thus, represent hydrophilic polyurethanes as promising synthetic matrices for cartilage regeneration.

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“…α-ω-cholesterol-modified poly(oxyethylene), that could be inserted into the hydrophobic region of the lipid bilayer and interconnected the cells to form gels with a storage modulus around 100<G'<500 Pa[159]. More recently, Raghavan et al synthesized hydrophobically-modified chitosan or alginate polymers that were anchored on the membrane of HUVECs via hydrophobic effect and capable of gelling the suspensions (1<G'<10 Pa), without affecting their viability[160].…”

mentioning

confidence: 99%

Cell membrane engineering with synthetic materials: Applications in cell spheroids, cellular glues and microtissue formation

Amaral

Pasparakis

2019

Acta Biomaterialia

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Biologically inspired materials with tunable bio-and physicochemical properties provide an essential framework to actively control and support cellular behavior. Cell membrane remodeling approaches benefit from the advances in polymer science and bioconjugation methods, which allow for the installation of un-/natural molecules and particles on the cells' surface. Synthetically remodeled cells have superior properties and are under intense investigation in various therapeutic scenarios as cell delivery systems, bio-sensing platforms, injectable biomaterials and bioinks for 3D bioprinting applications. In this review article, recent advances in the field of cell surface remodeling via biochemical means and the potential biomedical applications of these emerging cell hybrids are discussed.

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Reversible gelation of cells using self-assembling hydrophobically-modified biopolymers: towards self-assembly of tissue

Cited by 26 publications

References 25 publications

Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine

Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine

Hydrophilic polyurethane matrix promotes chondrogenesis of mesenchymal stem cells

Cell membrane engineering with synthetic materials: Applications in cell spheroids, cellular glues and microtissue formation

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