Reviving the Weizmann process for commercial n-butanol production

Nguyen, Ngoc-Phuong-Thao; Raynaud, Céline; Meynial-Salles, Isabelle; Soucaille, Philippe

doi:10.1038/s41467-018-05661-z

Cited by 94 publications

(72 citation statements)

References 46 publications

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“…To solve the problem of insufficient biomass in continuous cultivations, growth needs to be uncoupled from the liquid feed flow rate. Uncoupling can be done by regulating the cell concentration in a continuous culture equipped with a cell retention technique or by immobilization of the cells [ 86 , 114 , 210 , 222 , 338 ]. The topics of cell retention and immobilization are described in the following sections.…”

Section: Continuous Fermentation Methodsmentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

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The sustainable production of solvents from above ground carbon is highly desired. Several clostridia naturally produce solvents and use a variety of renewable and waste-derived substrates such as lignocellulosic biomass and gas mixtures containing H2/CO2 or CO. To enable economically viable production of solvents and biofuels such as ethanol and butanol, the high productivity of continuous bioprocesses is needed. While the first industrial-scale gas fermentation facility operates continuously, the acetone–butanol–ethanol (ABE) fermentation is traditionally operated in batch mode. This review highlights the benefits of continuous bioprocessing for solvent production and underlines the progress made towards its establishment. Based on metabolic capabilities of solvent producing clostridia, we discuss recent advances in systems-level understanding and genome engineering. On the process side, we focus on innovative fermentation methods and integrated product recovery to overcome the limitations of the classical one-stage chemostat and give an overview of the current industrial bioproduction of solvents.

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Section: Continuous Fermentation Methodsmentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

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“…ABE fermentation is a process for producing acetone, butanol, and ethanol, which is a representative industrial application of Clostridium strains including C. acetobutylicum [ 94 , 95 ]. Some efforts have been made to produce such non-native chemicals in other acetogens, such as C. aceticum , C. ljungdahlii , and A. woodii [ 47 , 51 , 64 , 90 , 96 ].…”

Section: Engineering Of Acetogens For Biochemical Productionmentioning

confidence: 99%

Synthetic Biology on Acetogenic Bacteria for Highly Efficient Conversion of C1 Gases to Biochemicals

Jin

Bae

Song

et al. 2020

IJMS

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Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen. Acetogenic bacteria (acetogens) have emerged as an alternative solution to recycle C1 gases by converting them into value-added biochemicals using the Wood-Ljungdahl pathway. Despite the advantage of utilizing acetogens as biocatalysts, it is difficult to develop industrial-scale bioprocesses because of their slow growth rates and low productivities. To solve these problems, conventional approaches to metabolic engineering have been applied; however, there are several limitations owing to the lack of required genetic bioparts for regulating their metabolic pathways. Recently, synthetic biology based on genetic parts, modules, and circuit design has been actively exploited to overcome the limitations in acetogen engineering. This review covers synthetic biology applications to design and build industrial platform acetogens.

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“…Low cell density fermentation is a disadvantage of clostridia compared with other industrial strains, such as yeast and lactobacillus. As to solventogenic clostridia, high cell density fermentation is necessary to develop effective continuous butanol production with better volumetric productivity (Nguyen et al, 2018).…”

Section: Reducing Cell Energy State Accelerated Glucose Utilization Amentioning

confidence: 99%

Enforcing ATP hydrolysis enhanced glycolysis and promoted solvent production under anaerobic conditions

Dai¹,

Zhu²,

Dong³

et al. 2020

Preprint

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Background The intracellular ATP level, an indicator of cellular energy state, plays a critical role in regulating the intracellular metabolic activities. The classical ATP paradox that the glycolytic rate negatively correlates with the intracellular ATP level was well studied. Reducing intracellular ATP level could stimulate the glycolytic rate, thereby enhancing products accumulation in aerobic conditions. However, there are limited studies about the effect of reducing cellular ATP level on anaerobic glycolysis and products formation.Results Taking anaerobic ABE (acetone-butanol-ethanol) fermentation by Clostridium acetobutylicum as a model, we reduced the intracellular ATP level by introducing an independent ATP hydrolysis module of F 1 -ATPase. We found that the low intracellular ATP level in C. acetobutylicum compared to that in aerobic microbes, was further reduced and the glucose uptake was remarkably enhanced, achieving a 78.8% increase of volumetric glucose utilization rate. The total solvent production was improved with increase in productivity (46.5%), titer (9.9%) and yield (14.5%) compared to control, with an early onset of solventogenesis and a shortened fermentation period. Consistently, genome-scale metabolic modeling revealed the elevated metabolic fluxes through glycolysis, TCA cycle, and pyruvate metabolism in F 1 -ATPase overexpression strain.Conclusions Decreasing the cellular energy level could further enhance the glycolytic rate at anaerobic condition and lead to significantly improvement on solvents synthesis. Our findings provide a novel strategy for improving ABE fermentation performance, which is beneficial for industrial Weizmann process. Engineering intracellular ATP level can thus be considered as a promising tool to enhance the efficiency of other anaerobic fermentation processes.

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Reviving the Weizmann process for commercial n-butanol production

Cited by 94 publications

References 46 publications

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Synthetic Biology on Acetogenic Bacteria for Highly Efficient Conversion of C1 Gases to Biochemicals

Enforcing ATP hydrolysis enhanced glycolysis and promoted solvent production under anaerobic conditions

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