Rheological behavior and microstructure of Pickering emulsions based on different concentrations of gliadin/sodium caseinate nanoparticles

Abstract

The properties of Pickering emulsion stabilized by different gliadin/sodium caseinate nanoparticle (Gli/CAS NPs) concentrations were investigated through physical stability, rheological properties, and microstructure observation. The results suggested that the higher the particle concentration, the smaller the size of the emulsion. The Pickering emulsion with 4% Gli/ CAS NPs displayed the smallest particle size and higher stability. All the Pickering emulsion samples showed pseudoplastic behavior which is regulated through NPs concentration and oil fractions. Frequency sweep and large deformation rheology showed that the prepared Pickering emulsions shared dominant solid characteristic which was mainly determined by the particle network in the continuous phase. CLSM and Cryo-SEM observation showed that Gli/CAS particles adsorbed on the oil-water interface and formed film structures. As Gli/CAS NPs increased, the surface film becomes denser which prevents the coalescence of adjacent oil droplets. The unadsorbed particles in the continuous phase formed a three-dimensional net structure which improved the stability and viscoelastic properties of Pickering emulsion. This work showed that the microstructure and rheological properties of Pickering emulsions could be regulated by particle concentration, which might provide interesting features for various industrial applications.

Formation, structure and stability of high internal phase Pickering emulsions stabilized by BSPI-C3G covalent complexes

“…4 C. For all of the samples, G' was always larger than G'', showing frequency dependence, which indicated that the Pickering emulsion might form a high internal phase gel structure. The interconnected network formed between the adsorbed and without adsorbed protein particles in the emulsions also improved the elasticity of the emulsion ( Xu et al, 2021 ). With the increase of BSPI-C3G particle concentration, the elasticity of emulsion gradually increased and then remained at a relatively high level, which may be due to the increase in the number of droplet–droplet interactions, indicating that BSPI-C3G particle-stabilized Pickering emulsion with an interfacial three-dimensional network promoted the formation of the elastic gel-emulsion network ( Liu et al, 2020 ).…”

Fabrication of Antioxidant Pickering Emulsion Based on Resveratrol-Grafted Zein Conjugates: Enhancing the Physical and Oxidative Stability

“…There was no stratification in any of the emulsions with different oil phase volume fractions; they were still uniform milky white liquids ( Figure 3 C). As shown in Figure 4 , in the observation of optical microstructure, the increased oil phase volume fraction led to an increase in emulsion droplet size and uneven distribution, which was due to the increase in the oil phase volume fraction, and oil droplets tended to aggregate and form a lipid layer [ 42 ]. The droplet size of the emulsions with 5% and 10% volume fractions of the oil phase did not change significantly after 15 days of storage.…”

Synthesis of polymeric nanoparticles by double emulsion and pH-driven: encapsulation of antibiotics and natural products for combating Escherichia coli infections

“…Protein variants, drawn from diverse sources like mung beans (Mohammadian et al 2021), whey protein, notably sodium caseinate (Peng et al 2020;Xu et al 2021), and proteins derived from soy and wheat (Sun et al 2023a;Kevij et al 2020), are integral components in the process of nanoencapsulation employing the described method. Zein, valued for its versatility and cost-effectiveness, is a frequent choice as a polymer in fabricating PNPs utilizing the pH-driven technique.…”