Rheological, dielectric and diffusion analysis of mucin/carbopol matrices used in amperometric biosensors

Capra, Raúl; Baruzzi, Ana María; Quinzani, Lidia M.; Strumia, Miriam Cristina

doi:10.1016/j.snb.2007.01.022

Cited by 40 publications

(24 citation statements)

References 26 publications

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“…Nasal mucus consists of 95% water, 2.5% glycoproteins, 1-2% electrolytes etc. Glycoproteins are responsible for rheological properties of the mucus and contain sialic acid (pK a = 2.6) and sulfate groups making mucin anionic at neutral pH (5). Although several theories have attempted to explain bioadhesive and mucoadhesive phenomena, explanations based on surface energy thermodynamics are currently popular (3,6).…”

Section: Introductionmentioning

confidence: 99%

Surface Thermodynamics of Mucoadhesive Dry Powder Formulation of Zolmitriptan

et al. 2011

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Abstract. Microparticle powders for nasal delivery were formulated to contain the model drug, zolmitriptan, and varying proportions of different polymers. The objective of the study was to investigate the effects of these formulative parameters on the surface chemistry of the spray-dried microparticles and their potential for adhesion to the tested substrates, porcine mucin, and nasal tissue. The polymers used were chitosans of varying ionization states and molecular weights and hydroxypropyl methyl cellulose. The surface energies of the surfaces of the microparticles were determined using contact angle measurements and the van Oss model. The theory of surface thermodynamics was applied to determine the theoretical potential for the different materials to adhere to the substrates. It was found that the drug or polymers alone, as well as the various formulations, were more likely to adhere to mucin than to nasal tissue. Further, there was a trend for higher molecular weight chitosans to adhere better to the substrates than lower molecular weight chitosans. Similarly, adhesion was improved for formulations with a higher content of polymers. These theoretical predictions may be compared with further experimental results and be of use in making informed decisions on the choice of formulations for future expensive bio-studies.

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Section: Introductionmentioning

confidence: 99%

Surface Thermodynamics of Mucoadhesive Dry Powder Formulation of Zolmitriptan

et al. 2011

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“…It worth to mention that one of the best analyte detection limit was achieved for a sol-gel enzyme electrodes that immobilizes the enzyme into an albumin matrix [27,28]. Albumin is well characterized globular protein has several amino groups that could be linked to other species by using bi-functional molecules such as glutaraldehyde [29,30].…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of a lactate amperometric biosensor based on lactate oxidase cross-linked with polymeric matrixes

Romero¹,

Garay²,

Baruzzi³

2008

Sensors and Actuators B: Chemical

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“…The detection limit of biosensor was 3.0 M, which is better/lower than that of any other earlier electrochemical oxalate biosensor (Sakaa and Vallon, 1994;Shimohigoshi and Karobe, 1996;Reddy and Vadgama, 1997;Milardovic et al, 2000aMilardovic et al, ,b, 2008Perez et al, 2001;Hong et al, 2003;Fiorito and Torresi, 2004;Fiorito et al, 2005;Capra et al, 2007;Benavidez et al, 2009;Mishra et al, 2009;Chaudhary and Pundir, 2010) with the exception of DO metric oxalate biosensor based on PVC membrane (1 M) prepared in our laboratory (Pundir and Phaugat, 2009) which had a drawback, interference by aerobic O 2 . In order to check the repeatability and reproducibility of the method, the oxalate content in same urine sample was determined five times on a single day (within batch) and again after storage at −20 • C for one week (between batch).…”

Section: Evaluation Of Oxalate Biosensormentioning

confidence: 55%

“…Therefore the demand for a simple, sensitive, accurate and rapid method has arrived. Over the past few years, various electrochemical methods for determination of oxalate based on the oxalate oxidase (OxOx) have been reported (Rahni and Guilbault, 1986;Assolant et al, 1987;Dinckaya and Telefoncu, 1993;Sezginturk and Dinckaya, 2003;Sakaa and Vallon, 1994;Shimohigoshi and Karobe, 1996;Reddy and Vadgama, 1997;Milardovic et al, 2000aMilardovic et al, ,b, 2008Perez et al, 2001;Hong et al, 2003;Fiorito and Torresi, 2004;Fiorito et al, 2005;Capra et al, 2007;Benavidez et al, 2009;Mishra et al, 2009;Pundir and Phaugat, 2009;Chaudhary and Pundir, 2010). However these OxOx based biosensors have some common drawbacks such as unstability due to leaching of enzyme from membrane/support, low conductivity and interference in electron communication.…”

Section: Introductionmentioning

confidence: 99%