Rheological Investigation of the Gel Time and Shrinkage in Hybrid Organic/inorganic UV Curable Films

Nayinia, Mohsen Mohammad Raei; Bastani, Saeed; Moradian, Siamak; Croutxé‐Barghorn, Céline; Allonas, Xavier

doi:10.2494/photopolymer.29.105

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…By monitoring its rheological properties, we can investigate the materials’ curing process. As shown in Figure B, the developed oil had S-shaped time evolution curves of storage G ′ and loss G ″ modulus at 37 °C, which are similar to those of PDMS and photopolymerization materials . At the initial 33 min of the curing process, both storage G ′ and loss G ″ modulus were relatively steady and G ″ was greater than G ′, thereby indicating that the oil was liquid.…”

Section: Resultsmentioning

confidence: 60%

“…As shown in Figure 2B, the developed oil had S-shaped time evolution curves of storage G′ and loss G″ modulus at 37 °C, which are similar to those of PDMS and photopolymerization materials. 33 At the initial 33 min of the curing process, both storage G′ and loss G″ modulus were relatively steady and G″ was greater than G′, thereby indicating that the oil was liquid. Following that, the storage modulus increased perpendicularly and exceeded the loss modulus in 4 min, which was evidence of the transition process from the liquid to the solid state.…”

Section: Constituents and Physical Properties Of Thementioning

confidence: 94%

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Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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Matrix metalloproteinases (MMPs) play an important role in tumor progression. The study of dynamic MMPs activity at the single-cell level can dissect tumor heterogeneity in the time domain and facilitate finding out more efficient clinical solutions for tumor treatment. Due to the fluidity of the carrier oil, the existing droplet-based methods for single-cell MMP analysis rarely have the capability to track proteolytic assays in droplets continuously. Therefore, we describe a thermosetting oil for realtime monitoring of MMP assays in droplets, which can immobilize droplets by transforming into solid after droplet generation. The solidification of this oil can be accomplished in 33 min at 37 °C, basing on the hydrosilation of vinyl silicone oil and hydrosilicone oil without other inducers (e.g. UV, Ca 2+ ). Through monitoring the MMP assays of single cells, the reaction rates can be calculated according to real-time fluorescent curves, showing significant cell heterogeneity in MMP activity. Moreover, the dynamic MMP activity reveals that some of the A549 cells transiently secrete MMP. In conclusion, the thermosetting oil enables immobilize droplets to achieve real-time monitoring of single-cell proteolytic activity without impairing the flexibility of droplet microfluidics and has a potential in other cell-based assays for providing dynamic information at high resolutions.

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Section: Resultsmentioning

confidence: 60%

Section: Constituents and Physical Properties Of Thementioning

confidence: 94%

Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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“…As shown in Figure C, Oil A had the similar time evolution of storage G ′ and loss G ″ modulus to PDMS and photopolymerization materials . At the initial 25 min of the curing, both storage G ′ and loss G ″ modulus were relatively steady and G ″ was greater than G ′, thereby indicating the oil was liquid.…”

Section: Resultsmentioning

confidence: 80%

A Thermosetting Oil for Droplet‐Based Real‐Time Monitoring of Digital PCR and Cell Culture

Zhou

et al. 2018

Adv Funct Materials

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Real-time observation of small volumes helps to understand phenomena ranging from reaction kinetics to cellular heterogeneity. However, highthroughput real-time monitoring systems based on droplets are rarely reported due to droplet motion and coalescence. Herein, a thermosetting oil is developed as a continuous phase to solve these problems through transforming into elastic solid after droplet generation. The thermosetting oil consists of silicon oil, nonionic surfactant, vinyl silicone oil, hydrosilicone oil, and Pt catalyst. Using Pt as the catalyst, the polymerization of this mixture can be accomplished in 72 min and 37 s at 37 and 95 °C, respectively, based on the hydrosilation of vinyl silicone oil and hydrosilicone oil without other inducers (e.g., UV, Ca 2+ ). Droplet-based real-time monitoring of digital polymerase chain reaction (PCR) and cell culture is successfully achieved using this thermosetting oil, demonstrating that droplets motion and coalescence can be eliminated by the crosslinking material without the influence on bioreactions. In addition, the PCR products can be totally recovered from microcapsules created by this oil for further analysis. Thus, the newly developed oil allows real-timely monitoring individual droplets in a high-throughput manner, as well as has potential for other bioreactions and cell-based assays.

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“…Most photoinitiators work in the ultraviolet (UV) region of the spectrum. [14][15][16] However, the UV or near-UV blue light irradiation can damage the cells, and DNA and result in the cancerization of the skin. 17,18 Utilizing visible-light responsive photo-initiators for polymerization is hence safer.…”

Section: Introductionmentioning

confidence: 99%

Architecting oxidized alginate methacrylate hydrogels with tunable characteristics by altering the sequence of the cross-linking steps, methacrylation reaction time, and polymer concentration

2023

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In this study, biodegradable oxidized methacrylated alginate (OMA) hydrogels with controllable mechanical properties were engineered. An ionic and photo cross-linking combination was employed to fabricate dual cross-linked hydrogels. By altering the degree of methacrylation and polymer concentration, hydrogels with an elastic modulus of 4.85 ± 0.13 to 21.02 ± 0.91 kPa, controllable swelling, and degradation kinetics, and cross-link density in the range of 1.0 × 10−5 to 6.5 × 10−5 mol/cm3 were obtained. Moreover, evaluating the effect of cross-linking sequence on the hydrogels’ mechanical properties demonstrated that in comparison to the hydrogels fabricated by ionic cross-linking followed by photo-polymerization, hydrogels produced by photo-polymerization followed by ionic cross-linking retain a stiffer gel network with more compact structure. Cytocompatibility examination was performed via MTT assay against L929 fibroblasts, and all the hydrogel samples demonstrated high cell viability (>80%). The findings demonstrate the significant effect of the sequence of cross-linking as a novel tool to tune the OMA hydrogel’s final properties which can serve as a useful platform for tissue engineering applications.

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Rheological Investigation of the Gel Time and Shrinkage in Hybrid Organic/inorganic UV Curable Films

Cited by 7 publications

References 38 publications

Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells

Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells

A Thermosetting Oil for Droplet‐Based Real‐Time Monitoring of Digital PCR and Cell Culture

Architecting oxidized alginate methacrylate hydrogels with tunable characteristics by altering the sequence of the cross-linking steps, methacrylation reaction time, and polymer concentration

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