Rheology of native dextrans in relation to their primary structure

Sabatié, J.; Choplin, Lionel; Doublier, Jean‐Louis; Arul, Joseph; Paul, F.; Monsan, Pierre

doi:10.1016/0144-8617(88)90047-1

Cited by 28 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This type of behavior in IL agrees with a previous report on the in vitro enzymatic synthesis of dextran in an aqueous medium [20]. The steady-shear viscosity also exhibited the same tendency for these polymerization conditions as shown in Fig.…”

Section: Resultssupporting

confidence: 90%

Enzymatic synthesis of poly-l-lactide and poly-l-lactide-co-glycolide in an ionic liquid

Chanfreau

Mena

Porras-Domínguez

et al. 2009

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

The syntheses of poly-L-lactide (PLLA) and poly-L-lactide-co-glycolide (PLLGA) is reported in the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate [HMIM][PF(6)] mediated by the enzyme lipase B from Candida antarctica (Novozyme 435). The highest PLLA yield (63%) was attained at 90 degrees C with a molecular weight (M(n)) of 37.8 x 10(3) g/mol determined by size exclusion chromatography. This procedure produced relatively high crystalline polymers (up to 85% PLLA) as determined by DSC. In experiments at 90 degrees C product synthesis also occurred without biocatalyst, however, PLLA synthesis in [HMIM][PF(6)] at 65 degrees C followed only the enzymatic mechanism as ring opening was not observed without the enzyme. In addition, the enzymatic synthesis of PLLGA is first reported here using Novozyme 435 biocatalyst with up to 19% of lactyl units in the resulting copolymer as determined by NMR. Materials were also characterized by TGA, MALDI-TOF-MS, X-ray diffraction, polarimetry and rheology.

show abstract

Section: Resultssupporting

confidence: 90%

Enzymatic synthesis of poly-l-lactide and poly-l-lactide-co-glycolide in an ionic liquid

Chanfreau

Mena

Porras-Domínguez

et al. 2009

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…12,13,37 Molecular weights up to 2 million and concentration up to 30% w/w have been shown to have a Newtonian behavior. 1,8,43,48 The viscosity values we obtained compare well with literature data, Fig. 1.…”

Section: Discussionsupporting

confidence: 81%

Concentration and Time Effects of Dextran Exposure on Endothelial Cell Viability, Attachment, and Inflammatory Marker Expression In Vitro

2010

View full text Add to dashboard Cite

Dextran is commonly used to alter growth medium rheological properties for in vitro flow experiments in order to match physiological parameters. Despite its acceptance in literature, few studies have examined dextran effects on cells. In this study, we investigated changes in endothelial cell function due to dextran, under static and flow conditions, in a concentration and time-dependent manner. Dextran increased endothelial cell viability, decreased their ability to attach to culture plates and decreased leukocyte adhesion to endothelial cells. Under static conditions, dextran increased protein and mRNA expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in a concentration and time-dependent manner and caused the nuclear translocation of NF-kappaB. Steady laminar wall shear stress modulated the effects of dextran on ICAM-1, VCAM-1, and NF-kappaB expression in straight/tubular in vitro models. When the expression was normalized to their respective time matched static dextran control, it did not affect the ability to detect changes caused by shear on the mRNA expression of ICAM-1 and VCAM-1. This study demonstrates that dextran can alter endothelial cell function and therefore, caution is advised and time matched dextran controls are necessary when using dextran for dynamic cell studies.

show abstract

“…Specifically, dextran, which consists mainly of a-1,6 linked anhydroglucose unit and partly of a-1,2-, a-1,3-or a-1,4-linked side chains, has been used as plasma volume expander for several years (Chen, Jo, & Park, 1995;Sabatie et al, 1988). However, polysaccharide-based biopolymers have limitations for their application due to poor-long term stability, weak mechanical properties, and difficulty in processibility (Choi, Kim, & Park, 1999).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and aqueous dispersion of dextran-g-poly(1,4-dioxan-2-one) copolymers

Bajgai

Parajuli

et al. 2009

Carbohydrate Polymers

View full text Add to dashboard Cite

Rheology of native dextrans in relation to their primary structure

Cited by 28 publications

References 20 publications

Enzymatic synthesis of poly-l-lactide and poly-l-lactide-co-glycolide in an ionic liquid

Enzymatic synthesis of poly-l-lactide and poly-l-lactide-co-glycolide in an ionic liquid

Concentration and Time Effects of Dextran Exposure on Endothelial Cell Viability, Attachment, and Inflammatory Marker Expression In Vitro

Synthesis, characterization and aqueous dispersion of dextran-g-poly(1,4-dioxan-2-one) copolymers

Contact Info

Product

Resources

About