Rhizosphere bacterial and fungal communities during the growth of Angelica sinensis seedlings cultivated in an Alpine uncultivated meadow soil

Abstract

Background: Angelica sinensis seedlings are grown in alpine uncultivated meadow soil with rainfed agroecosystems to ensure the quality of A. sinensis after seedling transplantation. The aim was to investigate the rhizosphere bacterial and fungal communities during the growth stages of A. sinensis seedlings. Methods: The bacterial and fungal communities were investigated by HiSeq sequencing of 16S and 18S rDNA, respectively. Results: Proteobacteria and Bacteroidetes were bacterial dominant phyla throughout growth stages. Fungal dominant phyla varied with growth stages, dominant phyla Ascomycota and Chytridiomycota in AM5, dominant phyla Basidiomycota, Ascomycota and Zygomycota in BM5, and dominant phyla Basidiomycota and Ascomycota in CM5. There was no significant variation in the alpha-diversity of the bacterial and fungal communities, but significant variation was in the beta-diversity. We found that the variation of microbial community composition was accompanied by the changes in community function. The relative abundance of fungal pathogens increased with plant growth. We also identified the core microbes, significant-changing microbes, stage-specific microbes, and host-specific microbes. Plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the key divers to microbial community composition. Conclusions: Our findings reported the variation and environmental drivers of rhizosphere bacterial and fungal communities during the growth of A. sinensis seedlings, which enhance the understanding of the rhizosphere microbial community in this habitat.

Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis

“…The site of cultivation is mountainous with meadow soil and a rainfed agroecosystem. It has a cool and semi-humid climate with an annual average temperature of 5–6 °C, approximately 2219 h annual sunshine, 90–120 frost-free days per year, and 451.4–817.8 mm of annual rainfall, mainly from June to September 60 .…”

Insights into the mechanism of the effects of rhizosphere microorganisms on the quality of authentic Angelica sinensis under different soil microenvironments

“…Proteobacteria and bacteroidetes were the dominant bacterial phyla throughout the growing season of A. sinensis , whereas fungal dominant phyla varied with growth periods. Furthermore, the plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the main factors contributing to the variation in microbial community composition of A. sinensis [ 15 ]. The most recent study on plant–microbe interactions mainly focused on succession cropping obstacles of medicinal plants, responses of plant–microbe to environmental stress, changes in rhizosphere nutrition conditions, root system biomass, and growth but provided little information on the accumulation of active constituents under different soil microenvironments [ 16 ].…”

Insights into the deriving of rhizosphere microenvironments and its effects on the growth of authentic Angelica sinensis seedlings under continuous monoculture

“…It has been reported that soil microorganisms play a key role in the transformation of material and energy in the micro-ecological environment of plant roots (Banerjee et al, 2016). The rhizosphere cultivable microbial community, fungal and bacterial diversity, and core bacterial community were signi cantly affected by continuous cultivation of A. sinensis seedlings (An et al, 2020). Imbalanced ammonia-oxidizing bacterial populations may lead to reduced growth of A. sinensis (Zhang et al, 2016).…”