Ribonucleases during cold acclimation in winter and spring wheats

Spanò, Carmelina; Crosatti, Cristina; Pacchini, Roberta; Meletti, Paolo; Grilli, Isa

doi:10.1016/s0168-9452(02)00026-2

Cited by 9 publications

(9 citation statements)

References 23 publications

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“…RNases are secreted from the aleurone layer of cereal grains and have key role in the hydrolysis and mobilization of RNA in the -Filho et al 1999). Activity of RNases gets modulated under variety of stressful conditions like heat, chilling, salinity, and heavy metals (Shah and Dubey 1995;Chang and Gallie 1997;Gomes-Filho et al 1999;Spanò et al 2002;Mishra and Dubey 2006;Maheshwari and Dubey 2007). Proteases are expressed during seed development as well as germination (Zhang and Jones 1999).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

Maheshwari

Dubey

2008

Acta Physiol Plant

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When the seeds of two rice cvs. Malviya-36 and Pant-12 were germinated up to 120 h in the presence of 200 and 400 lM NiSO 4 , a significant reduction in the germination of seeds occurred. Seeds germinating in the presence of 400 lM NiSO 4 showed about 12-20% decline in germination percent, about 20-53% decline in lengths and about 8-34% decline in fresh weights of roots and shoots at 120 h of germination. Ni 2+ exposure of germinating seeds resulted in apparent increased levels of RNA, soluble proteins, and free amino acids in endosperms as well as embryo axes. A 400 lM Ni 2+ treatment led to about 58-101% increase in the level of soluble proteins and about 39-107% increase in the level of free amino acids in embryo axes at 96 h of germination. Activities of ribonuclease and protease declined significantly with increasing levels of Ni 2+ treatment. Isoenzyme profile of RNase as revealed by activity staining indicated decline in the intensities of 3-4 preexisting enzyme isoforms in embryo axes of both the rice cultivars and disappearance of one of the two isoforms in endosperms of cv. Pant-12 due to 400 lM Ni 2+ treatment. Results suggest that the presence of high level of Ni 2+ in the medium of germinating rice seeds serves as a stress factor resulting in decreased hydrolysis as well as delayed mobilization of endospermic RNA and protein reserves and causing imbalance in the level of biomolecules like RNA, proteins, and amino acids in growing embryo axes. These events would ultimately contribute to decreased germination of rice seeds in high Ni 2+ containing environment.

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Section: Discussionmentioning

confidence: 99%

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

Maheshwari

Dubey

2008

Acta Physiol Plant

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“…Acidic, neutral salt-stimulated and neutral salt-inhibited RNases and nucleases were analysed, as several RNases and SSPNs are known to exist in plants, and their activity changes in relation to physiological and stress conditions (Blank and McKeon, 1991;Chang and Gallie, 1997;Spanò et al, 2002).…”

Section: Article In Pressmentioning

confidence: 99%

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Spanò

Buselli

Castiglione

et al. 2007

Journal of Plant Physiology

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“…Extraction of ribonucleases was made according to Spanò et al [25]. Enzymatic extract was stored in liquid nitrogen until use.…”

Section: Rnase Extraction and Electrophoresismentioning

confidence: 99%

“…Electrophoresis was carried out according to Blank and McKeon [26] with minor modifications [25] using 12.5% SDS-PAGE gels containing 2.4 mg mL À1 Torula yeast RNA. Following electrophoresis, gels were washed, specifically incubated and stained as in Spanò et al [25].…”

Section: Rnase Extraction and Electrophoresismentioning

confidence: 99%

“…Following electrophoresis, gels were washed, specifically incubated and stained as in Spanò et al [25]. In particular gels were incubated for 90 min at 37 C in: 100 mM imidazole buffer pH 8.0 for the salt-inhibited neutral RNases; 100 mM imidazole buffer pH 8.0 containing 200 mM KCl for the salt-stimulated neutral RNases; 50 mM sodium acetate buffer pH 5.7 for the acidic RNases.…”

Section: Rnase Extraction and Electrophoresismentioning

confidence: 99%

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Responses to desiccation injury in developing wheat embryos from naturally- and artificially-dried grains

Spanò

Bottega

Grilli

et al. 2011

Plant Physiology and Biochemistry

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Ribonucleases during cold acclimation in winter and spring wheats

Cited by 9 publications

References 23 publications

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

Inhibition of ribonuclease and protease activities in germinating rice seeds exposed to nickel

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Responses to desiccation injury in developing wheat embryos from naturally- and artificially-dried grains

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