Ribosomal and mitochondrial DNA polymorphisms in Colletotrichum gloeosporioides isolated from tropical fruits

Hodson, A.; Mills, Peter R.; Brown, Averil E.

doi:10.1016/s0953-7562(09)81130-1

Cited by 46 publications

(18 citation statements)

References 25 publications

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“…The low level of genetic polymorphism observed in subgroup CA-clonal containing only isolates from strawberry compared to subgroup CA-variable is consistent with a possible specialization of these isolates on strawberry, as suggested previously for C. acutatum isolates from almond (8) or for C. gloeosporioides isolates from mango (13,14). Since C. acutatum is reported to be a polymorphic, polyphageous fungus (29), the low level of genetic polymorphism observed among isolates in group CA-clonal may thus be a consequence of a recent epidemic of clonal line(s) pathogenic on strawberry.…”

Section: Discussionsupporting

confidence: 89%

Genetic Diversity and Pathogenic Variability Among Isolates of Colletotrichum Species from Strawberry

Denoyés-Rothan¹,

Guérin²,

Délye³

et al. 2003

Phytopathology®

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Ninety-five isolates of Colletotrichum including 81 isolates of C. acutatum (62 from strawberry) and 14 isolates of C. gloeosporioides (13 from strawberry) were characterized by various molecular methods and pathogenicity tests. Results based on random amplified polymorphic DNA (RAPD) polymorphism and internal transcribed spacer (ITS) 2 sequence data provided clear genetic evidence of two subgroups in C. acutatum. The first subgroup, characterized as CA-clonal, included only isolates from strawberry and exhibited identical RAPD patterns and nearly identical ITS2 sequence analysis. A larger genetic group, CA-variable, included isolates from various hosts and exhibited variable RAPD patterns and divergent ITS2 sequence analysis. Within the C. acutatum population isolated from strawberry, the CA-clonal group is prevalent in Europe (54 isolates of 62). A subset of European C. acutatum isolates isolated from strawberry and representing the CA-clonal and CA-variable groups was assigned to two pathogenicity groups. No correlation could be drawn between genetic and pathogenicity groups. On the basis of molecular data, it is proposed that the CA-clonal subgroup contains closely related, highly virulent C. acutatum isolates that may have developed host specialization to strawberry. C. gloeosporioides isolates from Europe, which were rarely observed were either slightly or nonpathogenic on strawberry. The absence of correlation between genetic polymorphism and geographical origin in Colletotrichum spp. suggests a worldwide dissemination of isolates, probably through international plant exchanges.

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Section: Discussionsupporting

confidence: 89%

Genetic Diversity and Pathogenic Variability Among Isolates of Colletotrichum Species from Strawberry

Denoyés-Rothan¹,

Guérin²,

Délye³

et al. 2003

Phytopathology®

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“…Because of its economic importance as a plant pathogen, Colletotrichum has received a lot of attention from numerous authors, and several molecular methods have been developed to detect it in plant tissue (2,8,15) or to determine phylogenetic relationships (6,9,20,21). However, the taxonomy of Colletotrichum is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Morphological Identification ofColletotrichumSpecies of Clinical Interest

2004

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Colletotrichum species have caused human infections in recent years. Because of the difficulties in recognizing them in vitro, we have designed a quick and unambiguous molecular test, based on the amplification of a specific fragment of the internal transcribed spacer 1 region, to distinguish any Colletotrichum isolate from other fungi, including the common pathogenic species. Analysis of the sequences of the ribosomal DNA (rDNA) fragment showed sufficient variability to clearly separate the five species of Colletotrichum that are of clinical interest, i.e., Colletotrichum coccodes, C. crassipes, C. dematium, C. gloeosporioides, and C. graminicola. Sequencing of the D1-D2 region of the large-subunit rDNA gene also supported these results. Additionally, we reviewed the most suitable morphological characteristics for the in vitro identification of these increasingly important opportunistic fungi.Hyphomycetes are the most common pathogenic moulds, but coelomycetes can also be found in human infections. These infections mainly occur in immunosuppressed hosts, although healthy individuals are also occasionally affected when the epithelial barrier is disturbed. Coelomycetes are asexual fungi that produce their fertile hyphae in specialized structures called conidiomata. They mainly produce two types of conidiomata: (i) pycnidia, which are more or less spherical fruit bodies with an opening only at the apex and a layer of the conidiogenous cells lining the inner cavity wall, and (ii) acervuli, which are cup-shaped fruiting bodies whose fertile hyphae form a palisade on the surface of the conidiomata. In a recent review of infections by coelomycetes, 11 genera were reported, but those most commonly found in clinical specimens are Colletotrichum, Nattrassia, and Phoma (24).The genus Colletotrichum is one of the most important genera of plant pathogens. It has a worldwide distribution but is found mainly in subtropical and tropical regions (1). Colletotrichum species cause economically significant diseases of plants (generally referred to as anthracnoses) that affect cereals and grasses, legumes, vegetables, and perennial crops, including fruit trees (1). The key morphological features which identify the genus are its acervular conidiomata, often with setae (dark-pigmented, unbranched, thick-walled sterile hyphae usually pointed at the tip), producing elongated slimy conidia, and the presence of appressoria (thick-walled swellings at the end of a hypha or germ tube useful for attaching the fungus to the host surface before penetration of the tissue). The genus Colletotrichum encompasses numerous species, and the key criterion for their identification is based mainly on determining the plant host. In the most recent review of the genus, Sutton (23) accepted 39 species but indicated that the separation or recognition of some of these species is unclear.Five species of Colletotrichum have been reported to cause infections in humans. They are Colletotrichum coccodes, C. crassipes, C. dematium, C. gloeosporioides, and C. grami...

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“…These procedures together with the targets they probe comprise isozyme analyses of 11, 13, or 19 enzyme systems on the protein level (Bonde et al 1991;Meijer et al 1994;Kaufman and Weideman 1996); sequence analyses of the PCR-amplifi ed internal transcribed spacer (ITS) region of rDNA (Mills et al 1992;Rehner and Uecker 1994;Sherriff et al 1994Sherriff et al , 1995Sreenivasaprasad et al 1996;Johnston and Jones 1997;Zhang et al 1997a;Uddin et al 1998); amplifi ed fragment length polymorphisms (AFLPs) of genomic DNA (Majer et al 1996;O'Neill et al 1997); restriction fragment length polymorphisms (RFLPs) of rDNA, mitochondrial DNA (mtDNA), and genomic DNA (Braithwaite et al 1990;Vaillancourt and Hanau 1992;Hodson et al 1993;Alahakoon et al 1994;Riccioni et al 2003); RFLPs and DNA-DNA hybridization (Liyanage et al 1992;Bernstein et al 1995;Brown et al 1996); primer PCR-initiated random amplifi ed polymorphic DNAs (RAPDs) or AT-rich DNA analyses of genomic DNA (Welsh and McClelland 1990;Williams et al 1990;Guthrie et al 1992;Vaillancourt and Hanau 1992;Freeman et al 1993;Blakemore et al 1994;Trigiano et al 1995;Fernandez and Hanlin 1996;Freeman and Katan 1997;Mackie and Irwin 1998;Chakraborty et al 1999); species-specifi c primer analysis of rDNA-ITS1 region Adaskaveg and Hartin 1997;Freeman et al 2000); and high mobility group (HMG)-encoding sequence of the MAT1-2 mating type sequence …”

Section: Molecular Techniques and Targets In Coelomycetesmentioning

confidence: 99%

Coelomycete systematics with special reference to Colletotrichum

Thaung

2008

Mycoscience

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Coelomycete systematics with special reference to ColletotrichumImperfecti, which is polyphyletic, artifi cial, and untenable or redundant (Taylor 1995). Form-taxa are classifi ed solely on their conidial ontogeny, morphology, or ecology. Such classifi catory systems are exclusive, restrictive, of limited use, and not indicative of clear relationships among taxa. Primarily, they serve nomenclature and identifi cation purposes only.Abstract Morphological and molecular data of coelomycetes are analyzed. Taxonomic tools and species concepts are explored. The bimodal systematics approach is emphasized. A comprehensive case study on Colletotrichum is included.

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Ribosomal and mitochondrial DNA polymorphisms in Colletotrichum gloeosporioides isolated from tropical fruits

Cited by 46 publications

References 25 publications

Genetic Diversity and Pathogenic Variability Among Isolates of Colletotrichum Species from Strawberry

Genetic Diversity and Pathogenic Variability Among Isolates of Colletotrichum Species from Strawberry

Molecular and Morphological Identification ofColletotrichumSpecies of Clinical Interest

Coelomycete systematics with special reference to Colletotrichum

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