Ribosomal RNA and nucleolar proteins from the oocyte are to some degree used for embryonic nucleolar formation in cattle and pig

Maddox-Hyttel, Poul; Svarcova, O.; Laurinčík, Jozef

doi:10.1016/j.theriogenology.2007.03.015

Cited by 24 publications

(26 citation statements)

References 42 publications

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“…The vacuolation of NPBs, penetration of rDNA and subsequent rRNA synthesis, and DFC formation in the body of NPB in nucleoli of ruminant type are completely different to that in porcine without vacuolation of NPBs, attachment of rDNA, and initiation of DFC formation in the periphery of NPBs. However, these structural differences do not explain the initiation of nucleoli formation in ovine-porcine iSCNT embryos (different types of nucleoli) and the absence of nucleoli in mouse-porcine iSCNT embryos (similar types of nucleoli (Flechon & Kopecny 1998, Maddox-Hyttel et al 2007.…”

Section: Discussionmentioning

confidence: 96%

“…In mammals, this was confirmed by the demonstration that RNA polymerase I as well as other key nucleolar proteins, upstream binding factor (UBF) and topoisomerase I, engaged in transcription of the rDNA, and fibrillarin , which is involved in early processing of rRNA, appeared in embryos at the time of EGA (Maddox-Hyttel et al 2007. The rRNA gene activation and the associated nucleolus formation are suggested as markers of the major EGA in mammalian embryos (Bjerregaard et al 2007).…”

Section: Introductionmentioning

confidence: 89%

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Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 89%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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“…B23 recruited in pronuclei at 6 hpa, and the signal became stronger at 8 hpa because this protein was inherited and reused in early cleavage (33). There were no obvious differences among the four groups.…”

Section: Resultsmentioning

confidence: 88%

rRNA Genes Are Not Fully Activated in Mouse Somatic Cell Nuclear Transfer Embryos

Zheng

Jia

Bou

et al. 2012

Journal of Biological Chemistry

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Background: The nucleolus always shows delayed development and malfunction in somatic cell nuclear transfer (NT) embryos. Results: NT embryos showed low rDNA activity and developmental competence when donor cells with low rDNA activity were used. Conclusion: rDNA reprogramming efficiency in NT embryos was determined by the rDNA activity in donor cells from which they derived. Significance: Developmental potential of NT embryos with rDNA activity in the donor cells was correlated.

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“…(2) The oocyte acquires meiotic competence and the nucleolus is disassembled during the growth phase. (2) The oocyte, enclosed in a large follicle, is then ready to respond to the preovulatory gonadodropin surge. At this point, it resumes meiosis and the nucleolar remnant is dissolved.…”

Section: Development Of the Nucleolus During Oocyte Growthmentioning

confidence: 99%

The nucleolus of the maternal gamete is essential for life

Lefèvre

2008

BioEssays

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The mammalian oocyte is a round cell arrested at prophase I of meiosis. It is characterized by the presence of a large nucleus, called the germinal vesicle, in the middle of which is the nucleolus. Before it can be fertilized, the oocyte must resume meiosis, enter metaphase II and be ovulated. The nucleolus is dissolved during this process. However, the nucleoli of the male and female pronuclei in the zygote are both of maternal origin. A recent paper1 demonstrates that the maternal nucleolus, together with other nucleoplasmic elements, is essential for early embryonic development. These nucleolar and nucleoplasmic factors remain undetermined.

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Ribosomal RNA and nucleolar proteins from the oocyte are to some degree used for embryonic nucleolar formation in cattle and pig

Cited by 24 publications

References 42 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

rRNA Genes Are Not Fully Activated in Mouse Somatic Cell Nuclear Transfer Embryos

The nucleolus of the maternal gamete is essential for life

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