Ribosomal RNA phylogeny of bacterial and fungal pathogens of Agriotes wireworms

Leclerque, Andreas; Mitkovets, P. V.; Fătu, C.; Schuster, Christina; Kleespies, Regina

doi:10.1007/s10340-012-0450-1

Cited by 6 publications

(1 citation statement)

References 30 publications

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“…Mortality from Metarhizium anisopliae and Metarhizium brunneum (Clavicipitaceae) has been reported for many elaterid species (78) (Figure 1k), and under laboratory conditions these fungi can drastically reduce larval populations. Screening of pathogenic strains has led to promising isolates, and laboratory and field testing is underway to control various Agriotes species (6,78,99). Beauveria bassiana (Cordycipitaceae) has been isolated from Conoderus spp., Agriotes mancus, and Hypnoidus bicolor and tested on several Canadian Prairies species (78).…”

Section: Fungimentioning

confidence: 99%

Biology, Ecology, and Control of Elaterid Beetles in Agricultural Land

Traugott

Benefer²,

Blackshaw³

et al. 2015

Annu. Rev. Entomol.

136

118

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Wireworms, the larvae of click beetles (Coleoptera: Elateridae), have had a centuries-long role as major soil insect pests worldwide. With insecticidal control options dwindling, research on click beetle biology and ecology is of increasing importance in the development of new control tactics. Methodological improvements have deepened our understanding of how larvae and adults spatially and temporarily utilize agricultural habitats and interact with their environment. This progress, however, rests with a few pest species, and efforts to obtain comparable knowledge on other economically important elaterids are crucial. There are still considerable gaps in our understanding of female and larval ecology; movement of elaterids within landscapes; and the impact of natural enemies, cultivation practices, and environmental change on elaterid population dynamics. This knowledge will allow generation of multifaceted control strategies, including cultural, physical, and chemical measures, tailored toward species complexes and crops across a range of appropriate spatial scales.

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Section: Fungimentioning

confidence: 99%

Biology, Ecology, and Control of Elaterid Beetles in Agricultural Land

Traugott

Benefer²,

Blackshaw³

et al. 2015

Annu. Rev. Entomol.

136

118

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Multilocus Sequence Analysis (MLSA) of ‘Rickettsiella agriotidis’, an Intracellular Bacterial Pathogen of Agriotes Wireworms

Schuster

Kleespies

Ritter³

et al. 2012

Curr Microbiol

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Wireworms, the polyphagous larvae of click beetles belonging to the genus Agriotes (Coleoptera: Elateridae) are severe and widespread agricultural pests that affect numerous crops globally. A new bacterial specimen identified in diseased wireworms had previously been shown by microscopy and 16S ribosomal RNA (rRNA) gene-based phylogenetic reconstruction to belong to the taxonomic genus Rickettsiella (Gammaproteobacteria) that comprises intracellular bacteria associated with and typically pathogenic for a wide range of arthropods. Going beyond these earlier results obtained from rRNA phylogenies, multilocus sequence analysis (MLSA) using a four marker scheme has been employed in the molecular taxonomic characterization of the new Rickettsiella pathotype, referred to as 'Rickettsiella agriotidis'. In combination with likelihood-based significance testing, the MLSA approach demonstrated the close phylogenetic relationship of 'R. agriotidis' to the pathotypes 'Rickettsiella melolonthae' and 'Rickettsiella tipulae', i.e., subjective synonyms of the nomenclatural type species, Rickettsiella popilliae. 'R. agriotidis' forms, therefore, part of a Rickettsiella pathotype complex that most likely represents the species R. popilliae. As there are currently no genetic data available from the R. popilliae type strain, the respective assignment cannot be corroborated directly. However, an alternative taxonomic assignment to the species Rickettsiella grylli has been positively ruled out by significance testing. MLSA has been shown to provide a more powerful tool for taxonomic delineation within the genus Rickettsiella as compared to 16S rRNA phylogenetics. However, the limitations of the present MLSA scheme for the sub-species level classification of 'R. agriotidis' and further R. popilliae synonyms has been critically evaluated.

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