Rifampicin resistance in Mycobacterium tuberculosis in Iran: a two-centre study

Bahraminia, Farhad; Zangiabadian, Moein; Nasiri, Mohammad Javad; Fattahi, Mohammad; Goudarzi, Mehdi; Ranjbar, Reza; Fooladi, Abbas Ali Imani

doi:10.1016/j.nmni.2021.100909

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Automated DNA sequencing has identified more than 50 mutations in this region, with most involving point mutations in codons 516, 526, or 531 (Morgan et al 2005 ). Research indicates that over 90% of RIF-resistant TB is also resistant to isoniazid (INH), making RIF resistance an effective strong marker for MDR-TB (Bahraminia et al 2021 ; Mousavi Sagharchi and Mahmoudi Nasab 2023 ).…”

Section: Genome Sequence and Mutationsmentioning

confidence: 99%

New insight in molecular detection of Mycobacterium tuberculosis

Mousavi-Sagharchi,

Afrazeh,

Seyyedian-Nikjeh

et al. 2024

AMB Expr

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Mycobacterium tuberculosis, the causative agent of tuberculosis, is a pathogenic bacterium that has claimed millions of lives since the Middle Ages. According to the World Health Organization’s report, tuberculosis ranks among the ten deadliest diseases worldwide. The presence of an extensive array of genes and diverse proteins within the cellular structure of this bacterium has provided us with a potent tool for diagnosis. While the culture method remains the gold standard for tuberculosis diagnosis, it is possible that molecular diagnostic methods, emphasis on the identification of mutation genes (e.g., rpoB and gyrA) and single nucleotide polymorphisms, could offer a safe and reliable alternative. Over the past few decades, as our understanding of molecular genetics has expanded, methods have been developed based on gene expansion and detection. These methods typically commence with DNA amplification through nucleic acid targeted techniques such as polymerase chain reaction. Various molecular compounds and diverse approaches have been employed in molecular assays. In this review, we endeavor to provide an overview of molecular assays for the diagnosis of tuberculosis with their properties (utilization, challenges, and functions). The ultimate goal is to explore the potential of replacing traditional bacterial methods with these advanced molecular diagnostic techniques.

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Section: Genome Sequence and Mutationsmentioning

confidence: 99%

New insight in molecular detection of Mycobacterium tuberculosis

Mousavi-Sagharchi,

Afrazeh,

Seyyedian-Nikjeh

et al. 2024

AMB Expr

View full text Add to dashboard Cite

show abstract

“…TB is a significant health problem in Iran. According to the WHO report in 2021 on the epidemiological categorization of countries, territories, and areas based on incidence per 100,000 population in 2019, Iran ranked in the lower-moderate category ( Bahraminia et al., 2021 ; Doosti et al., 2023a ; Khademi & Sahebkar, 2021 ; Organization, 2021 ). Iran has implemented a TB control program since the 1970s, but the disease persists, particularly in disadvantaged and marginalized populations.…”

Section: Introductionmentioning

confidence: 99%