Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis

Chi, Peidong; Liu, Yijun; Huang, Yuhua; Mao, Min; Wang, Yu; Li, Zhiming; Li, Jian

doi:10.1002/cam4.3540

Cited by 3 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flow cytometry (FCM) combined with monoclonal immunofluorescence antibodies has emerged as a valuable technique in the field of medicine for the quantitative detection of cell surface, intracellular antigens, and membrane receptors [14][15][16][17][18][19][20]. Compared to immunohistochemistry (IHC), FCM offers several advantages, including rapid detection speed, high sensitivity, and cost-effective sample analysis.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Fine-Needle Biopsy (FNB) versus Fine-Needle Aspiration (FNA) Combined with Flow Cytometry in the Diagnosis of Deep-Seated Lymphoma

Yang,

Aruna,

Cheng

et al. 2023

Diagnostics

View full text Add to dashboard Cite

Evidence comparing ultrasound endoscopy-guided fine-needle biopsy (EUS-FNB) with EUS-guided fine-needle aspiration (EUS-FNA) in deep-seated lymphoma tissue sampling is insufficient. This study aims to evaluate the diagnostic efficacy of immunohistochemistry (IHC) or flow cytometry (FCM) on specimens obtained from EUS-FNB and EUS-FNA in the diagnosis and staging of deep-seated lymphomas. This real-world, dual-center study prospectively evaluated all eligible specimens from patients who underwent EUS-FNB/FNA over an 8-year period. 53 patients were enrolled, with 23 patients in the EUS-FNB group and 30 patients in the EUS-FNA group. FNB yielded specimens with longer core tissues (0.80 mm [0.55, 1.00] vs. 0.45 mm [0.30, 0.50], p = 0.009) and higher scores of specimen adequacy [4 (3.75, 4.00) vs. 3 (1.00, 4.00), p = 0.025]. Overall analysis revealed that the diagnostic accuracy of IHC based on specimens acquired from EUS-FNB was significantly higher than that of EUS-FNA (91.30% vs. 60.00%, p = 0.013). After controlling confounding factors including lesion size and endoscopists, EUS-FNB with IHC maintained a higher-level diagnostic accuracy compared to EUS-FNA (OR = 1.292 [1.037–1.609], p = 0.023). When FCM was additionally used to analyze the specimen acquired from EUS-FNA, the diagnostic yield was significantly improved (ROC AUC: 0.733 vs. 0.550, p = 0.015), and the AUC of FNB alone or combined with FCM was 0.739 and 0.761. Conclusions: FNB needles generate higher histopathological diagnostic accuracy and specimen quality than FNA for the deep-seated lymphoma. Though the application of FCM significantly improves the diagnostic efficacy of EUS-FNA, FNB was still the preferred diagnostic modality with a shorter procedure time, comparable diagnostic accuracy, and better cost-effectiveness.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Fine-Needle Biopsy (FNB) versus Fine-Needle Aspiration (FNA) Combined with Flow Cytometry in the Diagnosis of Deep-Seated Lymphoma

Yang,

Aruna,

Cheng

et al. 2023

Diagnostics

View full text Add to dashboard Cite

show abstract

Incorporation of ultrasound-guided core biopsy with flow cytometry to assist the diagnosis of cervical lymphoma

Chen

Huang

et al. 2022

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

Analysis of flow cytometry data from ultrasound‐guided lymph node biopsies with two types of needles

Jin,

Jiang,

Jiang

et al. 2024

Int J Lab Hematology

View full text Add to dashboard Cite

BackgroundIn this study, we combined two techniques, ultrasound‐guided needle biopsy and flow cytometry (FCM), to explore their value in patients with enlarged lymph nodes.MethodsWe compared the results of 198 needle biopsies on FCM and pathology. Forty‐two were done by (fine needle aspiration, FNA), and the remaining 156 with (core needle biopsy, CNB), in 36 of 156 patients, a FNA was performed in the same lymph node after completion of the CNB. Except for five types of pathological entities, the rest were differentiated only detected or undetected tumours as the outcome distinction.ResultsAmong the 198 needle biopsies, 13 were inadequate specimens, while the remaining 185 had pathological findings, including 47 benign and 138 neoplastic findings. Thirty‐six patients underwent puncture with both FNA and CNB, both needles produced identical results by FCM, but more cells were obtained by FNA. Among the pathologically positive results, there were 23 missed diagnoses in FCM, in contrast, evidence of tumours was observed in the FCM images of 15 needle biopsies that reported benign or findings that were inconsistent with pathology, and the final diagnosis was consistent with the FCM in 10 cases. FCM detected haematolymphoid tumours with a sensitivity of 87.8% and a specificity of 91.9%.ConclusionThe combination of FCM and ultrasound‐guided lymph node needle biopsy can quickly provide guidance for clinical decision‐making. We recommend that all lymph node needle biopsies be sent for FCM, the specimen can be obtained by the last puncture with FNA.

show abstract

Rinsing sampling of core needle biopsy for flow cytometric analysis: A favorable method for lymphoma diagnosis

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 3 publications

References 28 publications

Comparison of Fine-Needle Biopsy (FNB) versus Fine-Needle Aspiration (FNA) Combined with Flow Cytometry in the Diagnosis of Deep-Seated Lymphoma

Comparison of Fine-Needle Biopsy (FNB) versus Fine-Needle Aspiration (FNA) Combined with Flow Cytometry in the Diagnosis of Deep-Seated Lymphoma

Incorporation of ultrasound-guided core biopsy with flow cytometry to assist the diagnosis of cervical lymphoma

Analysis of flow cytometry data from ultrasound‐guided lymph node biopsies with two types of needles

Contact Info

Product

Resources

About