RNA-cleaving DNAzymes as a diagnostic and therapeutic agent against antimicrobial resistant bacteria

Towards the development of a DNA automaton: modular RNA-cleaving deoxyribozyme logic gates regulated by miRNAs

“…In this study we have created six DNA logic gates that could find their utility in the diagnosis and therapy of genetic diseases. 5,43 As the basic structural element of our DNA logic gates, we have opted for the RNA cleaving biDz 10-23 for its high multiple turnover catalytic activity.…”

MicroRNA therapeutics and nucleic acid nano-delivery systems in bacterial infection: a review

“…For example, the development of a series of novel antimicrobial drugs with established modes of action in terms of inhibition of bacterial RNA polymerase transcription complexes, 137 and RNA-cleaving DNAzyme (RCD), a short catalytically active nucleic acid, have also been applied for the treatment of drug-resistant bacteria. 138 More and more strong evidence has shown that miRNA, one kind of small RNA, can play an important role in numerous biological processes including cell proliferation, apoptosis and other disease progresses which is involved in infection by various pathogens including viruses, parasites and bacteria. [139][140][141] miR-146a/b, miR-21, miR-155, miR-125, miR-17, let-7 family and other miRNAs are extensively investigated in the previous studies involved in innate immune response in multiple steps of bacterial infection (Fig.…”

Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA

“…The 8–17 DNAzyme has become a model system in the field of catalytic DNA, and it is active in the presence of divalent metal ions including Pb 2+ , Zn 2+ , Cd 2+ , Mg 2+ , and Ca 2+ . It contains a small catalytic core which is capable of efficiently catalyzing the cleavage reaction of RNA substrates . To build on their bioassay capabilities, DNAzymes have been used to construct signal amplification cascades to further improve the limit of detection, where target concentrations are low. − In a recent study, DNAzyme structures, referred to as Subzymes, were attached to magnetic microparticles for regulating DNAzyme activity in response to nucleic acid targets .…”