RNA interference with the allatoregulating neuropeptide genes from the fall armyworm Spodoptera frugiperda and its effects on the JH titer in the hemolymph

Griebler, Manuela; Westerlund, Stephanie A.; Hoffmann, Klaus H.; Meyering-Vos, Martina

doi:10.1016/j.jinsphys.2008.04.019

Cited by 79 publications

(70 citation statements)

References 62 publications

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“…α-Tubulin dsRNA was the least toxic and caused mortality up to 30% only. The reported effects of siRNA-mediated gene silencing is limited to reduced larval growth in H. armigera (Kumar et al 2009), reduction in the oviposition rates in Spodoptera fugiperda (Griebler et al 2008) and decrease in the target mRNA levels in Epiphyas postvittana (Turner et al 2006) and Tribolium castaneum (Bucher et al 2002;Tomoyasu and Denell 2004). Contrary to the earlier reports, we observed more than 80% mortality of whiteflies by feeding them siRNA/ dsRNA of RPL9 and V-ATPase A subunit.…”

Section: Discussioncontrasting

confidence: 92%

RNA interference for the control of whiteflies (Bemisia tabaci) by oral route

et al. 2011

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RNA interference (RNAi)-mediated gene silencing was explored for the control of sap-sucking pest Bemisia tabaci, commonly known as whitefly. dsRNAs and siRNAs were synthesized from five different genes - actin ortholog, ADP/ATP translocase, alpha-tubulin, ribosomal protein L9 (RPL9) and V-ATPase A subunit. A simplified insect bioassay method was developed for the delivery of ds/siRNA through the oral route, and efficacy was evaluated. ds/siRNA caused 29-97% mortality after 6 days of feeding. Each insect ingested nearly 150 nl of insect diet per day, which contained a maximum of 6 ng of RNA. Knocking down the expression of RPL9 and V-ATPase A caused higher mortality with LC50 11.21 and 3.08 microg/ml, respectively, as compared to other genes. Semi-quantitative PCR of the treated insects showed significant decrease in the level of RPL9 and V-ATPase A transcripts. siRNAs were found stable in the insect diet for at least 7 days at the room temperature. Phloem-specific expression of dsRNAs of RPL9 and V-ATPase A in transgenic plants for the protection against whiteflies might be an interesting application of this technology.

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Section: Discussioncontrasting

confidence: 92%

RNA interference for the control of whiteflies (Bemisia tabaci) by oral route

et al. 2011

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“…We characterized previously a peptide with allatotropic activity from the fall armyworm, Spodoptera frugiperda (Abdel-latief et al, 2004), with high similarity in its primary structure to the Manse-AS, but without the disulfide bridge, characteristic of type C allatostatins. RNA interference studies showed that this peptide has an allatostatic action in last instar larvae of S. frugiperda, whereas gene silencing in young adult females led to a large decrease in the JH titer of the animals (Griebler et al, 2008), which is consistent with an allatotropic activity of the peptide. The dependence of the JH-regulating activity of type C AS on the rate of JH production has been found also in other insect species and for other allatoregulating peptides (Clark et al, 2008).…”

Section: Discussionsupporting

confidence: 57%

Neuropeptide regulators of the juvenile hormone biosynthesis (in vitro) in the beetle, Tenebrio molitor (Coleoptera, Tenebrionidae)

Abdel-latief

Hoffmann

2010

Arch Insect Biochem Physiol

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The genome of Tribolium castaneum encodes two allatostatin [AS type B; W(X)(6)Wamide and AS type C; PISCF-OH] and one allatotropin (AT) precursor, but no AS type A (FGLamide) (Tribolium Genome Sequencing Consortium, 2008: Nature 452:949-955). Here we studied the activity (in vitro) of peptides derived from these precursors on the synthesis/release of juvenile hormone (JH) III. The corpora cardiaca-corpora allata (CC-CA) complexes of adult females of another tenebrionid beetle, the mealworm Tenebrio molitor, were used. Incubating the gland complexes in a medium containing Trica-AS B3 peptide, we showed that the peptide has allatostatic function in T. molitor. The activity of the type C AS depended on the age of the test animals and their intrinsic rate of JH III biosynthesis. The Trica-AS C peptide inhibited the JH release from CA of 3-day-old females with a high intrinsic rate of JH synthesis, but activated JH release from the CA of 7-day-old females with a lower intrinsic rate of JH production. The allatotropin peptide (Trica-AT) also activated the JH release from the CA of 7-day-old females in a dose-dependent and reversible manner. Unexpectedly, a type A AS derived from the precursor of the American cockroach Periplaneta americana (Peram-AS A2b) inhibited the JH release from the CA of younger and older females in the concentration range of 10(-8) to 10(-4) M, and the effects were fully reversible in the absence of peptide. These data suggest a complex role of allatoactive neuropeptides in the regulation of JH III biosynthesis in beetles.

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“…Overall, the goal of this method is to provide researchers the ability to expand the timeframe during which reverse genetic analyses can be performed, further enabling research that will support the development of novel vector control strategies. Interestingly, experiments in other species, such as Rhodnius prolixus and Spodoptera frugiperda, reveal that gene silencing effects tend to be much greater when initiated during pre-adult stages 38,39 . During all stages of development, RNAi-mediated gene knockdown is subject to considerations regarding the rapidity and persistence of gene silencing, and the stability of proteins encoded by targeted genes.…”

Section: Discussionmentioning

confidence: 99%

RNAi Trigger Delivery into <em>Anopheles gambiae</em> Pupae

Regna

Harrison

Heyse

et al. 2016

JoVE

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RNA interference (RNAi), a naturally occurring phenomenon in eukaryotic organisms, is an extremely valuable tool that can be utilized in the laboratory for functional genomic studies. The ability to knockdown individual genes selectively via this reverse genetic technique has allowed many researchers to rapidly uncover the biological roles of numerous genes within many organisms, by evaluation of loss-of-function phenotypes. In the major human malaria vector Anopheles gambiae, the predominant method used to reduce the function of targeted genes involves injection of double-stranded (dsRNA) into the hemocoel of the adult mosquito. While this method has been successful, gene knockdown in adults excludes the functional assessment of genes that are expressed and potentially play roles during pre-adult stages, as well as genes that are expressed in limited numbers of cells in adult mosquitoes. We describe a method for the injection of Serine Protease Inhibitor 2 (SRPN2) dsRNA during the early pupal stage and validate SRPN2 protein knockdown by observing decreased target protein levels and the formation of melanotic pseudo-tumors in SRPN2 knockdown adult mosquitoes. This evident phenotype has been described previously for adult stage knockdown of SRPN2 function, and we have recapitulated this adult phenotype by SRPN2 knockdown initiated during pupal development. When used in conjunction with a dye-labeled dsRNA solution, this technique enables easy visualization by simple light microscopy of injection quality and distribution of dsRNA in the hemocoel.

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RNA interference with the allatoregulating neuropeptide genes from the fall armyworm Spodoptera frugiperda and its effects on the JH titer in the hemolymph

Cited by 79 publications

References 62 publications

RNA interference for the control of whiteflies (Bemisia tabaci) by oral route

RNA interference for the control of whiteflies (Bemisia tabaci) by oral route

Neuropeptide regulators of the juvenile hormone biosynthesis (in vitro) in the beetle, Tenebrio molitor (Coleoptera, Tenebrionidae)

RNAi Trigger Delivery into <em>Anopheles gambiae</em> Pupae

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