RNAi-mediated resistance to SMV and BYMV in transgenic tobacco

Abstract

Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV)are

Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco

“…The determination of the transgenic locus number through real-time PCR overcomes the problems linked to phenotypic segregation analysis (i.e., lack of detectable expression even when the transgenes are present) and it is possible to analyze hundreds of samples per day. These two advantages mean that real-time PCR is an efficient method for estimating gene copy numbers (Gadaleta et al 2011, Thu et al 2016, Giancaspro et al 2017. Real-time PCR is a fast and sensitive method, but its precision depends on a reliable one-copy reference gene, which was confirmed by the results from this study.…”

RNAi technology for management of banana bunchy top disease

“…Nevertheless, RNAi has proven to be a useful approach in preventing the expression of viruses in plants (Table 1). It has been successfully used in conferring substantial varying level of resistance to plant viruses such as Mungbean yellow mosaic India virus , Papaya ringspot virus , Soybean mosaic virus , Cucumber mosaic virus , and Cassava mosaic virus (Jia et al., 2017; Kumar, Tanti, Patil, Mukherjee, & Sahoo, 2017; Ntui et al., 2014, 2015; Thu et al., 2016). Similar promising results have been obtained in suppressing the expression of BBTV.…”