Robust measurement of vitamin A status in plasma and blood dried on paper

Huang, Yi; Clements, Peter R.; Gibson, Robert A.

doi:10.1016/j.plefa.2015.10.001

Cited by 16 publications

(25 citation statements)

References 12 publications

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“…A recent study reported that adding chelators (such as EDTA) to the DBS papers increased correlations between stored DBS samples and venous blood control samples markedly ( r > 0.97) [ 43 ]. Similar improvement was also observed when ascorbic acid was added to DBS filters and during extraction, to improve the stability of vitamin A [ 105 ].…”

Section: Methodological Challenges and Refinement In Lipid Profiling mentioning

confidence: 56%

“…However, there exists some controversy on the efficacy of sampling and extraction techniques [ 114 ]. A recent paper obtained a high correlation ( r = 0.97) between venous blood and DBS samples from healthy subjects ( n = 24) using acidic extraction [ 105 ]. Similar results were demonstrated 30 years ago, where the loss of vitamin A in serum samples was eliminated when adding ascorbic acid before extraction [ 115 ].…”

Section: Dbs Profiling Of Vitamins a And Dmentioning

confidence: 99%

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Biomarkers for nutrient intake with focus on alternative sampling techniques

et al. 2016

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Biomarkers of nutrient intake or nutrient status are important objective measures of foods/nutrients as one of the most important environmental factors people are exposed to. It is very difficult to obtain accurate data on individual food intake, and there is a large variation of nutrient composition of foods consumed in a population. Thus, it is difficult to obtain precise measures of exposure to different nutrients and thereby be able to understand the relationship between diet, health, and disease. This is the background for investing considerable resources in studying biomarkers of nutrients believed to be important in our foods. Modern technology with high sensitivity and specificity concerning many nutrient biomarkers has allowed an interesting development with analyses of very small amounts of blood or tissue material. In combination with non-professional collection of blood by finger-pricking and collection on filters or sticks, this may make collection of samples and analyses of biomarkers much more available for scientists as well as health professionals and even lay people in particular in relation to the marked trend of self-monitoring of body functions linked to mobile phone technology. Assuming standard operating procedures are used for collection, drying, transport, extraction, and analysis of samples, it turns out that many analytes of nutritional interest can be measured like metabolites, drugs, lipids, vitamins, minerals, and many types of peptides and proteins. The advantage of this alternative sampling technology is that non-professionals can collect, dry, and mail the samples; the samples can often be stored under room temperature in a dry atmosphere, requiring small amounts of blood. Another promising area is the potential relation between the microbiome and biomarkers that may be measured in feces as well as in blood.

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Section: Methodological Challenges and Refinement In Lipid Profiling mentioning

confidence: 56%

Section: Dbs Profiling Of Vitamins a And Dmentioning

confidence: 99%

Biomarkers for nutrient intake with focus on alternative sampling techniques

et al. 2016

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“…Therefore, the PLs, due to their lipophilic character, were likely not co-extracted with the RBP complex and hence, did not interfere with the analysis of retinol. As retinol is sensitive to oxidation, extraction solvents contained antioxidants such as ascorbic acid (AA), diethylenetriamine pentaacetic acid (DTPA), butylated hydroxytoluene (BHT) or hydroquinone (29,(47)(48)(49)(50).…”

Section: Sample Preparationmentioning

confidence: 99%

“…This approach is not compatible with the direct application of a drop of blood from the fingertip onto filter paper. As an alternative, Huang et al spiked increasing concentrations of retinol standard solution to already prepared and dried DBSs, after which the spiked spots were dried (49).…”

Section: Endogenous Analytesmentioning

confidence: 99%

Dried blood microsample-assisted determination of vitamins: Recent developments and challenges

Verstraete

Boffel

Stove

2020

TrAC Trends in Analytical Chemistry

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Although vitamin deficiencies are a well-known issue, monitoring of the vitamin status and diagnosis of vitamin deficiencies in remote regions is a complicated task. Dried blood microsampling, as an alternative sampling strategy, could offer a solution to the several drawbacks related to conventional venipuncture.Although highly relevant, the number of microsampling procedures that has been developed for assessing vitamins in dried blood samples is rather limited, indicating the challenging nature of the subject. This review discusses several challenges inherent to vitamin analysis in microsamples, thereby covering topics including sampling strategy, hematocrit (Hct) effect, sample preparation, calibration, assay sensitivity, stability, and clinical interpretation of the results.

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“…Diagnosis of recent alcohol uptake LC-MS/MS 20 14 Fluoxetine, norfluoxetine, reboxetine and paroxetine TDM, toxicological analysis and PK studies NICI-MS-MS 21 15 Gabapentine Preclinical and clinical PK studies LC-MS/MS 22 16 Gemifloxacin Preclinical PK studies HILIC with fluorescence detector 23 17 Linezolid TDM LC-MS/MS 24 18 Losartan Preclinical PK studies LC-MS/MS 25 19 Methadone TDM HPLC 26 20 Metoprolol Preclinical, clinical PK and doping studies LC-MS/MS 27 21 Metronidazole PK/PD studies in neonatal patients HPLC 28 22 Nevirapine and Efavirenz TDM LC-MS/MS 29 23 Nifedipine Photo-degradation experiments LC-MS/MS 30 24 Opiates, cocainics and amphetamines Illicit drugs determination in suspected cases of driving under the influence of drugs LC-MS/MS 31 25 Paclitaxel Preclinical PK studies LC-MS/MS 32 26 Propranolol PK studies in neonates or young infants LC-MS/MS 33 27 Quinine and 3-hydroxyquinine Clinical PK studies HPLC with fluorescence detection 34 28 Ranitidine TDM in pediatrics samples LC-MS/MS 35 29 Sulfadoxine and pyrimethamine TDM and epidemiological studies HPLC-UV 36 30 Tacrolimus, sirolimus, everolimus and cyclosporin A TDM LC-MS/MS 37 31 Vincristine Clinical pharmacological studies LC-MS/MS 38 32 Zidovudine Monitoring of zidovudine therapy among HIV-infected pregnant mothers and their newborns RIA 39 Filter Paper used in DUS: Whatmann 903 neonatal cards with 13 mm diameter discs and Whatmann no. 903 filter paper are used in DUS.…”

mentioning

confidence: 99%

Untitled

2018

IJPSR

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Bioanalysis is a sub-discipline of analytical chemistry covering the quantitative measurement of xenobiotics (drugs and their metabolites and biological molecules in unnatural locations or concentration) and biotics (macromolecules, proteins, DNA, large molecule drugs, metabolites) in biological system. The focus of bioanalysis in the pharmaceutical industry is to provide as quantitative measure of the active drug and/or its metabolite(s) for the purpose of pharmacokinetics, toxicokinetics, bioequivalence and exposure response (pharmacokinetics / pharmacodynamics studies). Over the past several years dried matrix spot (DMS) sampling technique has emerged as a pertinent method in both qualitative and quantitative bioanalysis context. There are many types of DMS techniques such as dried blood spot (DBS), dried plasma spot (DPS), dried urine spot (DUS) and dried breast milk spot (DBMS). The most commonly used technique is DBS wherein the blood sample is directly soaked on to a paper (with or without treatment) and after drying it can be analyzed by modern analytical, immunological or genomic detection systems. Several advantages of DMS techniques such as low sample requirement, transportation and storage without special treatment, better analytes stability, enhanced clinical cooperation in clinical trials and reduced unforeseeable exposure of biohazard to analysts, make it the most appropriate sampling technique for bioanalysis. This review illustrates the available information on DBS, DPS, DUS and DBMS methods which may serve for investigators in the field of bioanalysis. Further, the proficiency and appliance of DMS method in pharmacokinetic (PK), therapeutic drug monitoring (TDM), toxicokinetic (TK), metabolomic and disease diagnosis is explored.

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Robust measurement of vitamin A status in plasma and blood dried on paper

Cited by 16 publications

References 12 publications

Biomarkers for nutrient intake with focus on alternative sampling techniques

Biomarkers for nutrient intake with focus on alternative sampling techniques

Dried blood microsample-assisted determination of vitamins: Recent developments and challenges

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