Robust polyfunctional T-helper 1 responses to multiple fungal antigens from a cell population generated using an environmental strain of Aspergillus fumigatus

Gaundar, Shivashni S.; Clancy, Leighton; Blyth, Emily; Meyer, Wieland; Gottlieb, David

doi:10.3109/14653249.2012.704013

Cited by 30 publications

(36 citation statements)

References 25 publications

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“…The majority of the responding CD4 cells produced one cytokine; however, 2.2% to 6.6% and 0.7% to 2.7% of poly-functional double and triple cytokine-producing cells, respectively, were present in all cultures (Figure 2b). This is similar to our previous observations in A fumigatusspecific T-cell cultures [22]. TNF-α was the predominant Th1 cytokine produced except for A flavus-specific T cells, which most commonly produced IFN-γ alone or in combination with TNF-α, and L prolificans-specific T cells, which predominantly produced IL-2.Two A terreus, two F oxysporum, three F solani and two R oryzae cultures also demonstrated positive CD8 responses (0.5% to 3.2% of CD8 cells expressing Th1 cytokines) (data not shown).…”

Section: Generation Of Fungus-specific T-cell Culturessupporting

confidence: 93%

“…We previously showed that a water-soluble lysate of A fumigatus can induce proliferation in PBMC from healthy donors [22]. The lymphoproliferative activity of lysates of other fungal genera was assessed in four healthy donors.…”

Section: Lymphoproliferative Effect Of Fungal Lysatesmentioning

confidence: 99%

“…We previously demonstrated that A fumigatus-specific T cells can be expanded in vitro from the peripheral blood of healthy donors [22]. We adopted the same protocol to expand T cells specific to other fungi in this study.…”

Section: Generation Of Fungus-specific T-cell Culturesmentioning

confidence: 99%

“…We previously published a method to expand A fumigatus-specific T cells in vitro from the peripheral blood of normal donors [22].The expanded T cells produced Th1,Th17 and neutrophilic cytokines. Fungusspecific T-cell responses were major histocompatibility complex (MHC) class II-dependent.…”

Section: Introductionmentioning

confidence: 99%

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Stimulation with lysates of Aspergillus terreus, Candida krusei and Rhizopus oryzae maximizes cross-reactivity of anti-fungal T cells

et al. 2016

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Section: Generation Of Fungus-specific T-cell Culturessupporting

confidence: 93%

Section: Lymphoproliferative Effect Of Fungal Lysatesmentioning

confidence: 99%

Section: Generation Of Fungus-specific T-cell Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stimulation with lysates of Aspergillus terreus, Candida krusei and Rhizopus oryzae maximizes cross-reactivity of anti-fungal T cells

et al. 2016

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“…Aspergillus-specific T cells appeared to be safe, and a reduced mortality in this patient cohort compared a historical control group was reported. An alternative protocol for in vitro expansion of clinical doses of Aspergillus-reactive T cells from healthy donors with an environmental strain of A. fumigatus for was described by Gaundar et al [24].…”

Section: Discussionmentioning

confidence: 99%

Clinical-scale isolation of the total Aspergillus fumigatus–reactive T–helper cell repertoire for adoptive transfer

et al. 2015

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Prophylactic antigen‐specific T‐cells targeting seven viral and fungal pathogens after allogeneic haemopoietic stem cell transplant

et al. 2021

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Objectives. Adoptive immunotherapy using donor-derived antigen-specific T-cells can prevent and treat infection after allogeneic haemopoietic stem cell transplant (HSCT). Methods. We treated 11 patients with a prophylactic infusion of 2 9 10 7 cells per square metre donor-derived T-cells targeting seven infections (six viral and one fungal) following HSCT. Targeted pathogens were cytomegalovirus (CMV), Epstein-Barr virus (EBV), adenovirus, varicella zoster virus, influenza, BK virus (BKV) and Aspergillus fumigatus. Results. T-cell products were successfully generated in all patients with 10 products responsive to 6 or 7 infections. T-cell infusions were associated with increases in antigen-experienced activated CD8 + T-cells by day 30. CMV, EBV and BKV reactivation occurred in the majority of patients and was well controlled except where glucocorticoids were administered soon after T-cell infusion. Three patients in that circumstance developed CMV tissue infection. No patient required treatment for invasive fungal infection. The most common CMV and EBV TCR clonotypes in the infusion product became the most common clonotypes seen at day 30 post-T-cell infusion. Donors and their recipients were recruited to the study prior to transplant. Grade III/IV graft-versus-host disease developed in four patients. At a median follow-up of 390 days post-transplant, six patients had died, 5 of relapse, and 1 of multi-organ failure. Infection did not contribute to death in any patient. Conclusion. Rapid reconstitution of immunity to a broad range of viral and fungal infections can be achieved using a

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Robust polyfunctional T-helper 1 responses to multiple fungal antigens from a cell population generated using an environmental strain of Aspergillus fumigatus

Cited by 30 publications

References 25 publications

Stimulation with lysates of Aspergillus terreus, Candida krusei and Rhizopus oryzae maximizes cross-reactivity of anti-fungal T cells

Stimulation with lysates of Aspergillus terreus, Candida krusei and Rhizopus oryzae maximizes cross-reactivity of anti-fungal T cells

Clinical-scale isolation of the total Aspergillus fumigatus–reactive T–helper cell repertoire for adoptive transfer

Prophylactic antigen‐specific T‐cells targeting seven viral and fungal pathogens after allogeneic haemopoietic stem cell transplant

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