Role of Annexin A1 in mouse myoblast cell differentiation

Bizzarro, Valentina; Fontanella, Bianca; Franceschelli, Silvia; Pirozzi, Marinella; Christian, Helen C.; Parente, Luca; Petrella, Antonello

doi:10.1002/jcp.22178

Cited by 51 publications

(64 citation statements)

References 33 publications

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“…29 Cytosolic ANXA1 for example has been frequently implicated in cytoskeletal organization in several cellular models. 48,31,32,9,18,10 ANXA1 localization after hypoxia administration to PCa cell lines showed a noteworthy alteration of its expression profiles. The protein was indeed significantly expressed onto plasma membrane and in the extracellular environments over the time during hypoxia treatment.…”

Section: Discussionmentioning

confidence: 95%

“…48 Proteins were visualized using the chemioluminescence detection system (Amersham) after incubation with primary antibodies against ANXA1 (rabbit polyclonal; 1:10000; 71-3400, Invitrogen), a-tubulin (mouse monoclonal; clone DM1A; 1:1000; Sigma-Aldrich), E-cadherin (mouse monoclonal; clone 36/E-Cadherin; 1:10000; BD Transduction Laboratories), vimentin (mouse monoclonal; clone E-5; 1:1000; Santa Cruz Biotechnologies), HIF1-a (rabbit polyclonal; clone A300-286A; 1:5000; Bethyl Laboratories), Ezrin (mouse monoclonal; clone 3C12; 1:250; Santa Cruz Biotechnologies), Moesin (mouse monoclonal; clone E-10; 1:250; Santa Cruz Biotechnologies), CK8 (mouse monoclonal, clone M20; 1:1000; Santa Cruz Biotechnologies), CK18 (mouse monoclonal, clone C-04; 1:1000; Santa Cruz Biotechnologies), p-ERK (rabbit monoclonal, clone Aw39; 1:1000; Cells Signaling), ERK (mouse monoclonal, clone MK12; Cells Signaling), p-p38 (mouse monoclonal; clone D-8; 1:1000; Santa Cruz Biotechnologies), p38 (rabbit polyclonal; clone C-20; 1:1000; Santa Cruz Biotechnologies), b-actin (mouse monoclonal; clone C-4, 1:1000; Santa Cruz Biotechnologies). The blots were exposed to Las4000 (GE Healthcare Life Sciences) and the relative band intensities were determined using ImageQuant software (GE Healthcare Life Sciences).…”

Section: Western Blottingmentioning

confidence: 99%

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Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Bizzarro

Belvedere

Migliaro

et al. 2016

Cell Adhesion & Migration

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Annexin A1 (ANXA1) is a Ca 2C -binding protein overexpressed in the invasive stages of prostate cancer (PCa) development; however, its role in this tumor metastatization is largely unknown. Moreover, hypoxic conditions in solid tumors have been related to poor prognosis in PCa patients. We have previously demonstrated that ANXA1 is implicated in the acquisition of chemo-resistant features in DU145 PCa cells conferring them a mesenchymal/metastatic phenotype. In this study, we have investigated the mechanisms by which ANXA1 regulates metastatic behavior in LNCaP, DU145 and PC3 cells exposed to hypoxia. ANXA1 was differentially expressed by PCa cell lines in normoxia whereas hypoxic stimuli resulted in a significant increase of protein expression. Additionally, in low oxygen conditions ANXA1 was extensively secreted out-side the cells where its binding to formyl peptide receptors (FPRs) induced cell invasion. Loss and gain of function experiments performed by using the RNA interfering siANXA1 and an ANXA1 over-expressing plasmid (MF-ANXA1), also confirmed the leading role of the protein in modulating LNCaP, DU145 and PC3 cell invasiveness. Finally, ANXA1 played a crucial role in the regulation of cytoskeletal dynamics underlying metastatization process, such as the loss of adhesion molecules and the occurrence of the epithelial to mesenchymal transition (EMT). ANXA1 expression increased inversely to epithelial markers such as E-cadherin and cytokeratins 8 and 18 (CKs) and proportionally to mesenchymal ones such as vimentin, ezrin and moesin. Our results indicated that ANXA1 may be a key mediator of hypoxia-related metastasis-associated processes in PCa.

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Section: Discussionmentioning

confidence: 95%

Section: Western Blottingmentioning

confidence: 99%

Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Bizzarro

Belvedere

Migliaro

et al. 2016

Cell Adhesion & Migration

Self Cite

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“…ANXA1 is a calcium-and phospholipid-binding protein involved in regulating a broad range of cellular events, including migration in different types of cells such as myoblasts, leukemia, and breast cancer lines and endothelial cells (7,26,47,48). Moreover, a recent study showed that ANXA1 Ϫ/Ϫ mice are unable to heal the damage associated with indomethacin-induced gastric ulcers (30).…”

Section: Discussionmentioning

confidence: 99%

Annexin-1-mediated Endothelial Cell Migration and Angiogenesis Are Regulated by Vascular Endothelial Growth Factor (VEGF)-induced Inhibition of miR-196a Expression

Houle

Fournier

et al. 2012

Journal of Biological Chemistry

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Background: Annexin-A1 is an important regulator of VEGF-mediated endothelial cell migration and angiogenesis. Results: We found that miR-196a targets Annexin-A1 to inhibit VEGF-mediated cell migration and angiogenesis. Moreover, VEGF decreases miR-196a and increases Annexin-A1. Conclusion: VEGF-induced decrease of miR-196a expression may participate to the angiogenic switch. Significance: These results bring important new insights in understanding the mechanisms underlying angiogenesis-associated pathologies.

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“…These candidates are relevant to other associated diseases, the apoptotic pathway and synthetic substances in tissues (24)(25)(26). However, the development of these in the process of IgAN are completely undetermined, and therefore, these novel candidates require further investigation.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of renal tissue in IgA nephropathy with iTRAQ quantitative proteomics

et al. 2014

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Abstract. Immunoglobulin (Ig) A nephropathy (IgAN) is the most common form of glomerulonephritis. In clinical practice, it is difficult to monitor the repeating relapse in patients suffering from IgAN, which usually occurs within 10 years of end-stage renal disease. In order to identify and quantify the total protein content in the renal tissue of patients with IgAN, isobaric tags for relative and absolute quantification (iTRAQ) technology was performed. iTRAQ coupled with multiple chromatographic fractionation and tandem mass spectrometry was used to analyze the total protein of normal renal tissue in IgAN and healthy patients. The individual proteins were identified by the Mascot search engine and any that were differentially expressed were monitored. A total of 574 different proteins were identified, and 287 proteins were up-or downregulated by >1 fold alteration in levels. The results showed that iTRAQ-based quantitative proteomic technology for the identification and relative quantitation of the renal tissue proteome is efficiently applicable. The differential expression of the proteome profiles for IgAN patients was determined. Further studies using large cohorts of patient samples with long-term clinical follow-up data should be conducted to evaluate the usefulness of the pathogenesis and novel biomarker candidates of IgAN, which may develop a novel technique for the diagnosis of IgAN.

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Role of Annexin A1 in mouse myoblast cell differentiation

Cited by 51 publications

References 33 publications

Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Hypoxia regulates ANXA1 expression to support prostate cancer cell invasion and aggressiveness

Annexin-1-mediated Endothelial Cell Migration and Angiogenesis Are Regulated by Vascular Endothelial Growth Factor (VEGF)-induced Inhibition of miR-196a Expression

Comparative proteomic analysis of renal tissue in IgA nephropathy with iTRAQ quantitative proteomics

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