Role of cell contacts for differentiation of precursor cells of hematopoietic stroma in long-term bone marrow cultures

Gurevich, O. A.; Ni, Drize; Chertkov, I. L.

doi:10.1007/bf00830737

Cited by 1 publication

(1 citation statement)

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“…It was shown previously that murine MSCs lose their proliferative and differentiation potentials in the case of dissociation of bone marrow cells or adherent cell layers from long-term bone marrow cultures [19,20]. Dissociation of rabbit MMSCs did not affect their ability to regenerate bone.…”

Section: Discussionmentioning

confidence: 73%

Participation of Cultured Mesenchymal Multipotent Stromal Cells in Regeneration of a Large Persisting Defect of Rabbit Radius Bone

V.E.¹,

Shipounova²,

Sats³

et al. 2012

TOTERMJ

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Abstract:The aim of this work was to characterize the impact of autologous cultured mesenchymal multipotent stromal cells (MMSCs) on the repair of large persisting defect in rabbit radius bone. MMSCs were obtained from femoral bone marrow aspirate, cultivated during 2-3 passages and marked by lentiviral vectors containing genes encoding green or cherry fluorescent proteins 4-6 days before implantation. The implantation of MMSCs resulted in new bone formation within 12 weeks. Cultured MMSC differentiated into bone, cartilage and other connective tissues in vivo. Mineral matrix used as a carrier of MMSС was completely resorbed in 10 weeks. Implanted MMSCs bearing marking gene maintained newly formed bone and cartilage tissues for at least one year. Thus, cultured MMSCs preserve the ability to differentiate and maintain bone tissue for long-term period in vivo, suggesting their high proliferative potential. Therefore, they could be used in tissue engineering in the healing of large bone defects.

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Section: Discussionmentioning

confidence: 73%