Role of chondroitin sulfate–hyaluronan interactions in the viscoelastic properties of extracellular matrices and fluids

Nishimura, Masahiro; Yan, Weiqun; Mukudai, Yoshiki; Nakamura, Shigeo; Nakamasu, Kazuko; Kawata, Masatoshi; Kawamoto, Takeshi; Noshiro, Mitsuhide; Hamada, Takashi; Kato, Yukio

doi:10.1016/s0304-4165(97)00119-0

Cited by 67 publications

(45 citation statements)

References 38 publications

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“…Both the number and time of cells locomoting and their velocities were increased, resulting in increased migration speed ( Figure 4C). Haptokinetic migration on HA, but not on uncoated glass, was significantly reduced, although not completely blocked by anti-CD44 mAb Hermes-1, which is consistent with previously published data (Thomas et al, 1992;Goebeler et al, 1996).In vitro, HA spontaneously aggregates, forming multimeric branched strands (Scott et al, 1991), binds to fibrillar collagen (Turley et al, 1985), and is further multimerized and stabilized by cross-linking CS, resulting in increased biomechanical resistance of the lattice (Nishimura et al, 1998). For constitution of multicomponent lattices, HA and CS were used in concentrations found in interstitial tissues (see MATERIALS AND METHODS).…”

supporting

confidence: 90%

“…Cells were preincubated with blocking mAbs (anti-CD44 mAb Hermes-1 and anti-␤1 integrin mAb 4B4; 10 g/ml) or PBS, embedded within the different matrices, and mAb was additionally added to supernatant and matrix. by two-to threefold (Nishimura et al, 1998), avidly interacts with collagen substrate, and stabilizes the collagen triplehelical structure (Comper and Laurent, 1978). In the present study, addition of CS to collagen markedly increased collagen fiber bundling in the presence or absence of HA, as detected by phase-contrast and confocal reflection microscopy (Wolf and Friedl, unpublished observations).…”

Section: Discussionsupporting

confidence: 66%

“…Efficient interpenetration and immobilization of HA in collagen lattices are supported by ultracentrifugation data showing a considerably greater mechanical stability and resistance to compression in the presence of HA (Fessler, 1960). The polysaccharide CS additionally cross-links HA monomers to form stable heteroduplexes (Scott et al, 1992), thereby increasing HA polymer rigidity (Nishimura et al, 1998). CS at physiological concentrations (5-40 mg/ml), as detected in dermis or cartilage, does not self-assemble (Scott et al, 1991 and, but increases the viscosity of HA gels Figure 6.…”

Section: Discussionmentioning

confidence: 94%

“…In vitro, HA spontaneously aggregates, forming multimeric branched strands (Scott et al, 1991), binds to fibrillar collagen (Turley et al, 1985), and is further multimerized and stabilized by cross-linking CS, resulting in increased biomechanical resistance of the lattice (Nishimura et al, 1998). For constitution of multicomponent lattices, HA and CS were used in concentrations found in interstitial tissues (see MATERIALS AND METHODS).…”

Section: Ha Promotes Mv3 Cell Migration On 2-d Surface But Not In 3-dmentioning

confidence: 99%

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Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

Maaser

Wolf

Klein

et al. 1999

MBoC

116

119

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Haptokinetic cell migration across surfaces is mediated by adhesion receptors including ␤1 integrins and CD44 providing adhesion to extracellular matrix (ECM) ligands such as collagen and hyaluronan (HA), respectively. Little is known, however, about how such different receptor systems synergize for cell migration through three-dimensionally (3-D) interconnected ECM ligands. In highly motile human MV3 melanoma cells, both ␤1 integrins and CD44 are abundantly expressed, support migration across collagen and HA, respectively, and are deposited upon migration, whereas only ␤1 integrins but not CD44 redistribute to focal adhesions. In 3-D collagen lattices in the presence or absence of HA and cross-linking chondroitin sulfate, MV3 cell migration and associated functions such as polarization and matrix reorganization were blocked by anti-␤1 and anti-␣2 integrin mAbs, whereas mAbs blocking CD44, ␣3, ␣5, ␣6, or ␣v integrins showed no effect. With use of highly sensitive time-lapse videomicroscopy and computer-assisted cell tracking techniques, promigratory functions of CD44 were excluded. 1) Addition of HA did not increase the migratory cell population or its migration velocity, 2) blocking of the HA-binding Hermes-1 epitope did not affect migration, and 3) impaired migration after blocking or activation of ␤1 integrins was not restored via CD44. Because ␣2␤1-mediated migration was neither synergized nor replaced by CD44 -HA interactions, we conclude that the biophysical properties of 3-D multicomponent ECM impose more restricted molecular functions of adhesion receptors, thereby differing from haptokinetic migration across surfaces. INTRODUCTIONTumor cell invasion and migration are supported by different adhesion receptor systems, including integrins and CD44 (Stetler-Stevenson et al., 1993;Sherman et al., 1994; Friedl and Brö cker, 1999). Integrins and CD44 interact with an array of extracellular matrix (ECM) components, including collagen, fibronectin, laminin, vitronectin, and hyaluronan (HA), respectively (Etoh et al., 1992;Danen et al., 1993;Aznavoorian et al., 1996;Goebeler et al., 1996;Klominek et al., 1997), simultaneously acting as structural links from the cytoskeleton to the ECM as well as signaling molecules (Lokeshwar et al., 1994;Clark and Brugge, 1995;Entwistle et al., 1996;Cox and Huttenlocher, 1998).In human melanoma, the expression of ␣2␤1 integrin is correlated with metastatic behavior (Klein et al., 1991b) and is involved in melanoma cell migration on collagen surfaces (Etoh et al., 1992) and in the reorganization of collagen matrices (Klein et al., 1991b;Schiro et al., 1991;Riikonen et al., 1995). Likewise, ␣3␤1 and ␣v␤3 integrins mediate chemotactic and haptotactic motility toward their respective ligands collagen (Lauer et al., 1998) and vitronectin □ V Online version of this article contains video material. Online version available at www.molbiolcell.org. ‡ Corresponding author. E-mail address: peter.fr@mail.uniwuerzburg.de. Abbreviations used: CS, chondroitin sulfate; ECM, extracellular matri...

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supporting

confidence: 90%

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 94%

Section: Ha Promotes Mv3 Cell Migration On 2-d Surface But Not In 3-dmentioning

confidence: 99%

See 2 more Smart Citations

Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

Maaser

Wolf

Klein

et al. 1999

MBoC

116

119

View full text Add to dashboard Cite

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“…However, the charge density of CS is twice as high as that of HA due to the fact that both GlcA and GalN residues are anionic in a CS molecule while HA carries a single negative charge per disaccharide unit [29]. It has been suggested that CS may act as a viscosity modifier within the ECM, as it has been shown that the viscosity of chondroitin sulfate solutions on their own is very low, while the addition of CS into HA solution increases the viscosity of the HA [30]. It has also been suggested that CS participates in water uptake and nutrient absorption within the tissues [28].…”

Section: Chondroitin Sulfatementioning

confidence: 99%

Improving the outcomes of biopharmaceutical delivery via the subcutaneous route by understanding the chemical, physical and physiological properties of the subcutaneous injection site

Kinnunen

Mrsny

2014

Journal of Controlled Release

135

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Publisher's copyright statement: NOTICE: this is the author's version of a work that was accepted for publication in Journal of Controlled Release. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reected in this document. Changes may have been made to this work since it was submitted for publication. A denitive version was subsequently published in Journal of Controlled Release, 182, 2014Release, 182, , 10.1016Release, 182, /j.jconrel.2014.011. Additional information:Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. AbstractSubcutaneous (SC) injection is currently the most common route of self-administering biopharmaceuticals such as proteins and peptides. While pharmaceutical scientists have acquired great skill in identifying formulations for these as proteins and peptides with multi-year shelf life stability, the SC injection of these formulations can result in inconsistent or particularly low bioavailability outcomes. We hypothesise that upon injection, the chemical, physical and physiological properties of the subcutaneous tissue may play a crucial role in determining the therapeutic outcomes of SC injected biopharmaceuticals. We contend that physical and chemical stresses placed upon the injected protein or peptide as is transitions from the non-physiological environment of its formulation to the homeostatic conditions of the SC tissue can affect its fate following injection. In this mini-review we describe how events that occur to an injected protein or peptide during this post-injection transition period could affect the diffusion of bioactive material to blood capillaries and lymphatic vessels. With this in mind, we have reviewed the chemical, physical and physiological attributes of the SC tissue and collated studies on how these properties are known to affect protein stability and diffusional properties. Finally, examples where the understanding of the properties of the SC tissue when formulating for SC injected biopharmaceuticals has improved the predictability of drug delivery via the SC route are discussed, with the need for novel tools for rational and informed formulation development is highlighted.

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Benign hyperplasia of the human prostate is associated with tissue enrichment in chondroitin sulphate of wide size distribution

et al. 2000

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BACKGROUND Benign prostatic hyperplasia (BPH) involves qualitative and quantitative alterations in extracellular matrix (ECM) components affecting stromal‐epithelial interactions. Glycosaminoglycans (GAGs) are polysaccharide components of the ECM whose role in the development of BPH is under investigation. METHODS GAGs were extracted from human prostates of normal and BPH origin and were subsequently fractionated through DEAE‐sephacel anion exchange chromatography. The isolated GAG fractions were identified through electrophoresis on cellulose acetate membranes and treatment with GAG‐degrading enzymes of known specificity. Their size distribution was determined through gradient polyacrylamide gel electrophoresis. RESULTS Isolated prostatic GAGs included hyaluronic acid (HA), heparan sulphate (HS), and a mixture of dermatan sulphate (DS) and chondroitin sulphate (CS). The CS/DS ratio was significantly higher in hyperplastic as compared to normal prostates. A difference was also observed with respect to the apparent molecular mass of the DS‐CS mixture, which reflects the CS enrichment in BPH. GAGs isolated from hyperplastic prostates were more diverse in size as compared to the corresponding glycans from normal prostates. CONCLUSIONS The apparent increase in CS and decrease in DS content in prostates of patients with BPH is in good agreement with the pathological manifestation of increased cell proliferation in hyperplastic prostate tissue, since these glycan molecules have been reported to increase and decrease cell proliferation, respectively. Identification of the responsible enzymes involved in the homeostasis of CS and DS may provide alternative targets for pharmacological intervention. Prostate 44:104–110, 2000. © 2000 Wiley‐Liss, Inc.

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Role of chondroitin sulfate–hyaluronan interactions in the viscoelastic properties of extracellular matrices and fluids

Cited by 67 publications

References 38 publications

Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

Functional Hierarchy of Simultaneously Expressed Adhesion Receptors: Integrin α2β1 but Not CD44 Mediates MV3 Melanoma Cell Migration and Matrix Reorganization within Three-dimensional Hyaluronan-containing Collagen Matrices

Improving the outcomes of biopharmaceutical delivery via the subcutaneous route by understanding the chemical, physical and physiological properties of the subcutaneous injection site

Benign hyperplasia of the human prostate is associated with tissue enrichment in chondroitin sulphate of wide size distribution

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