Role of curcuminoids in ameliorating oxidative modification in β-thalassemia/Hb E plasma proteome

Weeraphan, Churat; Srisomsap, Chantragan; Chokchaichamnankit, Daranee; Subhasitanont, Pantipa; Hatairaktham, Suneerat; Charoensakdi, Ratiya; Panichkul, Narumol; Siritanaratkul, Noppadol; Fucharoen, Suthat; Svasti, Jisnuson; Kalpravidh, Ruchaneekorn W.

doi:10.1016/j.jnutbio.2012.02.008

Cited by 17 publications

(35 citation statements)

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“…We observed that antioxidant proteins, chaperone proteins, proteins involving in iron metabolism, hemoglobin subunit d, cathepsin S (an inflammatory marker), and erythroid proteins were consistently increased in quantity in HbE/ b-thalassemic patients across all 3 pooled samples (Table 2), and this was also observed in 6 individual samples (Table 4). Taken together, the observed alterations in protein content in the thalassemic EVs are consistent with the known increase in oxidative burden due to peripheral hemolysis reported in previous studies, 13,22,24 and this study has substantially extended the number of known proteins with an altered concentration in HbE/ b-thalassemic patients' EVs.…”

Section: Discussionsupporting

confidence: 91%

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

et al. 2018

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Key Points• Chaperones, antioxidants, ironsequestering proteins, and cathepsin S exhibited increased abundance in thalassemic EVs.• Haptoglobin and hemopexin are reduced in thalassemic patients' EVs, reflecting hemolysis. These could be used as clinical biomarkers.Hemoglobin E (HbE)/b-thalassemia has a wide spectrum of clinical manifestations that cannot be explained purely by its genetic background. Circulating extracellular vesicles (EVs) are one factor that likely contributes to disease severity. This study has explored the differences in protein composition and quantity between EVs from HbE/b-thalassemic patients and healthy individuals. We used tandem mass tag labeling mass spectrometry to analyze the EV proteins isolated from the plasma of 15 patients compared with the controls.To reduce biological variation between individuals, the EV proteins isolated from randomly assigned groups of 5 HbE/b-thalassemic patients were pooled and compared with 5 pooled age-and sex-matched controls in 3 separate experiments. Alpha hemoglobin-stabilizing protein had the highest fold increase. Catalase, superoxide dismutase, T-complex proteins, heat shock proteins, transferrin receptor, ferritin, and cathepsin S were also upregulated in thalassemic circulating EVs. Importantly, haptoglobin and hemopexin were consistently reduced in patients' EVs across all data sets, in keeping with the existing hemolysis that occurs in thalassemia. The proteomic data analysis of EV samples isolated from 6 individual HbE/b-thalassemic patients and western blotting results corroborated these findings. In conclusion, we have successfully identified consistent alterations of protein quantity between EVs from HbE/b-thalassemic and healthy individuals. This work highlights haptoglobin, hemopexin, and cathepsin S as potential clinically relevant biomarkers for levels of hemolysis and inflammation. Monitoring of these plasma proteins could help in the clinical management of thalassemia.

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Section: Discussionsupporting

confidence: 91%

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

et al. 2018

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“…Our findings were in good agreement with prior studies [20,31,32,33]. In a research performed by Thephinlap et al [31] iron-loaded thalassemic mice were intervened with 200 mg/kg b.w.…”

Section: Discussionsupporting

confidence: 93%

“…To our knowledge, still no human randomized clinical trial has been conducted in this topic. There is only one published study in which Weeraphan et al [20] applied proteomic methods to determine the protein profile and oxidative damage in 10 β-thalassemia patients before and after treatment with curcumin. They reported that daily intake of 500 mg curcuminoids for 12 months did not change the Hb and serum ferritin level.…”

Section: Discussionmentioning

confidence: 99%

“…Inconsistent results obtained in mentioned studies may be due to the different characteristics of the subject including normal healthy mice [19], subclinical iron-deficient [15,] and β-thalassemia major patients [20]. Furthermore, variation in curcumin dose, duration of the intervention, and methodology of the study could influence the outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, there is only one published study that investigated the effects of curcumin on the protein profile and oxidative damage in β-thalassemia patients applying proteomic techniques [20]. So, the current study is initiated to evaluate the efficacy of curcumin supplementation on markers of oxidative stress in β-thalassemia major patients.…”

Section: Introductionmentioning

confidence: 99%

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Benefits of Curcumin Supplementation on Antioxidant Status in β-Thalassemia Major Patients: A Double-Blind Randomized Controlled Clinical Trial

Nasseri

Mohammadi²,

Tamaddoni³

et al. 2017

Ann Nutr Metab

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Background: β-Thalassemia major, the most common inherited anemia in the world, is associated with imbalance in the oxidant-antioxidant system. The objective of this study was to evaluate the efficacy of curcumin supplementation on markers of oxidative stress in patients with β-Thalassemia. Methods: This double-blind randomized controlled clinical trial was performed on 61 β-thalassemia major patients. Subjects in the curcumin group received two 500 mg curcumin capsules daily and patients in the placebo group took 2 placebo capsules daily for 12 weeks. Dietary intakes and biochemical parameters were assessed at the beginning and the end of intervention. Results: At the end of the study, serum malondialdehyde (MDA), total and direct bilirubin significantly decreased (p = 0.002, p < 0.001, and p < 0.001, respectively) and total antioxidant capacity significantly increased (p = 0.005) in the curcumin group. Based on the analysis of covariance, a significant reduction in MDA, total and direct bilirubin was also detected in the curcumin group when compared to the placebo group (p = 0.001, p = 0.039, and p = 0.013, respectively). Changes in hemoglobin, serum iron, ferritin, catalase, and vitamin E were not significant in any of the 2 groups. Conclusions: Curcumin supplementation in combination with deferoxamin improved the antioxidant status in β-thalassemia major patients. Curcumin may be useful for the relief of metabolic complications in these patients.

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The regulatory role of curcumin on platelet functions

Tabeshpour

Hashemzaei

Sahebkar

2018

J of Cellular Biochemistry

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Curcumin, the main ingredient of Curcuma longa L., has been used as a spice and as a herbal medicine with different therapeutic characteristics for centuries in Asian countries. This phytochemical has been shown to possess beneficial antiplatelet activity that has introduced it as a promising candidate for the treatment of thromboembolism, atherothrombosis, and inflammatory diseases. Platelet dysfunction under different circumstances may lead to cardiovascular disease, and curcumin has been shown to have beneficial effects on platelet dysfunction in several studies. Therefore, this narrative review is aimed to summarize available evidence on the antiplatelet activity of curcumin and related molecular mechanisms for this activity.

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Role of curcuminoids in ameliorating oxidative modification in β-thalassemia/Hb E plasma proteome

Cited by 17 publications

References 44 publications

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

Quantitative proteomics of plasma vesicles identify novel biomarkers for hemoglobin E/β-thalassemic patients

Benefits of Curcumin Supplementation on Antioxidant Status in β-Thalassemia Major Patients: A Double-Blind Randomized Controlled Clinical Trial

The regulatory role of curcumin on platelet functions

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