Role of high-fat diet on letrozole-induced polycystic ovarian syndrome in rats

Begum, Nazia; Manipriya, Kandavalli; Veeresh, Bantal

doi:10.1016/j.ejphar.2022.174746

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…Hepatic steatosis also occurred in this group of mice. Our findings are in line with those of previous studies showing that the use of letrozole with a high-fat diet may induce or worsen the symptoms of PCOS [1,25]. More specifically, Xu et al noted that twelve weeks of administration of LET with a high-fat diet in female Sprague-Dawley rats induced anovulatory cycles and polycystic ovary morphology, body weight gain, elevated testosterone levels, abnormal glucose and lipid metabolism, as well as insulin resistance [27].…”

Section: Discussionsupporting

confidence: 93%

“…More specifically, Xu et al noted that twelve weeks of administration of LET with a high-fat diet in female Sprague-Dawley rats induced anovulatory cycles and polycystic ovary morphology, body weight gain, elevated testosterone levels, abnormal glucose and lipid metabolism, as well as insulin resistance [27]. Begum et al indicated that twelve weeks of administration of the LET+HF diet in Wistar female rats induced additional glucose intolerance [1]. It should, however, be highlighted that all PCOS symptoms seen in our study developed as early as week five of the experiment when LET+HF/HFr was administrated; moreover, hepatic steatosis also occurred.…”

Section: Discussionmentioning

confidence: 99%

“…Polycystic ovary syndrome (PCOS) is one of the most common hormonal disorders among women of reproductive age. It significantly impairs their fertility and increases the risk of obesity, type 2 diabetes, hyperlipidemia, and cardiovascular disease [1]. Although the exact cause of PCOS is unknown, recent reviews of the PCOS research have found that genetic susceptibility is associated with PCOS and that environmental factors-such as endocrine disruptors and poor diet-are likely to play an important role in the expression of those genetic traits [2].…”

Section: Introductionmentioning

confidence: 99%

“…Although there are inconclusive data on metabolic disorders resulting from exposure to LET [13][14][15], polycystic changes in ovaries have occurred and disturbed endocrine parameters have been observed; these include elevated levels of testosterone and luteinizing hormone and reduced levels of estradiol, progesterone, and follicle-stimulating hormone [16,17]. The results of earlier studies indicate that the addition of high-fat diet to the LET model affords good metabolic aberrations, along with ovarian cysts [1,18,19]. However, all these studies were conducted among adult female rodents.…”

Section: Introductionmentioning

confidence: 99%

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The Role of a High-Fat, High-Fructose Diet on Letrozole-Induced Polycystic Ovarian Syndrome in Prepubertal Mice

et al. 2022

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This study aims to investigate the effects of a high-fat, high-fructose (HF/HFr) diet on metabolic/endocrine dysregulations associated with letrozole (LET)-induced Polycystic Ovarian Syndrome (PCOS) in prepubertal female mice. Thirty-two prepubertal C57BL/6 mice were randomly divided into four groups of eight and implanted with LET or a placebo, with simultaneous administration of an HF/HFr/standard diet for five weeks. After sacrifice, the liver and blood were collected for selected biochemical analyses. The ovaries were taken for histopathological examination. The LET+HF/HFr group gained significantly more weight than the LET-treated mice. Both the LET+HF/HFr and the placebo-treated mice on the HF/HFr diet developed polycystic ovaries. Moreover the LET+HF/HFr group had significantly elevated testosterone levels, worsened lipid profile and indices of insulin sensitivity. In turn, the HF/HFr diet alone led to similar changes in the LET-treated group, except for the indices of insulin sensitivity. Hepatic steatosis also occurred in both HF/HFr groups. The LET-treated group did not develop endocrine or metabolic abnormalities, but polycystic ovaries were seen. Since the HF/HFr diet can cause substantial metabolic and reproductive dysregulation in both LET-treated and placebo mice, food items rich in simple sugar—particularly fructose—and saturated fat, which have the potential to lead to PCOS progression, should be eliminated from the diet of young females.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of a High-Fat, High-Fructose Diet on Letrozole-Induced Polycystic Ovarian Syndrome in Prepubertal Mice

et al. 2022

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“…Increased consumption of high-calorie foods in one's daily diet has been shown to cause various fertilization issues and reproductive dysfunction, such as poor oocyte quality, low blastocyst survival rates, abnormal embryonic cellular differentiation, and ovarian apoptosis, in addition to being a dangerous risk factor for cardiovascular disease, diabetes, osteoarthritis, hypertension, cancer, and metabolic disorders [11][12][13] . HFD is commonly known to associate with polycystic ovary syndrome (PCOS) with its characteristic ovarian fibrosis [14] . Mammalian oviducts, or human fallopian tubes, are an active anatomical region in which all new life in mammalian species begins.…”

Section: Introductionmentioning

confidence: 99%

Differential Staining Patterns for Evaluation of Ovarian and Oviducts Fibrosis Using the Polarized Light Microscope and the Digital Images Analysis

Ahmed

2022

Egyptian Journal of Zoology

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Several histological and immunohistochemical techniques are used in the diagnosis of fibrosis. To better visualization of collagen fibers in both normal and pathological tissues, new optical approaches are required. The present study aimed to investigate/compare the efficiency of various histological stains, using the traditional light microscope and the light polarizing microscope, to quantify/evaluate the ovarian and oviduct fibrosis induced by high-fat diet (HFD) consumption. Twenty-Four female albino rats (Rattus norvegicus albinus) were randomly allotted into 4 groups (n=6): control groups (fed on standard diet for 6 and 12 weeks), HFD1 group (fed on HFD for 6 weeks), and HFD2 group (fed on HFD for 12 weeks). The ovarian and oviduct fibrosis were investigated via histological stains including hematoxylin and eosin, Picrosirius Red, van Gieson, and Masson's trichrome stains using traditional light and polarizing light microscopes, in addition to immunohistochemical demonstrations of α-smooth muscle actin (α-SMA) and vimentin. Hydroxyproline and tumor necrosis factor-α (TNF-α) were also investigated biochemically in the ovarian and oviduct tissues. The results showed that the HFD induced ovarian and oviducts fibrosis in time-dependent manner with significant increases in hydroxyproline and TNF-α values, as well as immunoexpression of α-SMA and vimentin. Picrosirius red stain achieved ideal results in collagen demonstration when compared with van Gieson and Masson's trichrome stains. Picrosirius red stain in combination with the polarization microscope represented high sensitivity in collagen estimation in comparison with light microscopy. This work recommended using polarizing microscope as a tool to evaluate fibrosis of both the ovaries and the oviducts.

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