Role of Melatonin in Reproductive Seasonality in Buffaloes

Ramadan, Tamer

doi:10.5772/intechopen.69549

Cited by 10 publications

(8 citation statements)

References 65 publications

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“…A previous study has shown seasonal variation in protein contents in buffaloes where highest crude protein contents were observed in winter and vice versa (Ahmad et al, 2013). This could be due to lower concentration of prolactin in summer and shorter daylight, both of which leads to higher melatonin level (Ramadan, 2017). In our study, we also found higher casein level in BuMEC after melatonin treatment.…”

Section: Discussionsupporting

confidence: 59%

Effect of Melatonin on Casein Expression in Buffalo Mammary Epithelial Cells

Riaz¹

2018

PVJ

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The objectives of this study are to find out the effect of melatonin treatments (43µM & 430µM) on mRNA and protein expression of casein, and melatonin receptors in buffalo mammary epithelial cells (BuMEC) using reverse transcriptional polymerase chain reaction (qRT-PCR) and Western blotting. Furthermore, by using specific inhibitors against mTOR (Rapamycin) and Jak2-Stat5 pathway (AG490), effect of melatonin treatment on translational level of casein was measured. The results showed that melatonin treatment (430 µM) significantly up-regulated casein level both at transcriptional (P<0.001) and translational (P<0.05) levels. Melatonin treatment significantly up-regulated mRNA expression of (P<0.05) melatonin receptor 1a (MT1) but melatonin receptor 1b (MT2) did not significantly varied. In the presence of mTOR inhibitor, rapamycin, melatonin failed to up regulate (P<0.05) protein expression of casein but no significant difference was found in the level of casein in the presence of Jak2-Stat5 inhibitor (AG490). These results indicate the significance of melatonin in altering milk casein expression in mammary epithelial cells in buffalo. Keeping in view the seasonality of buffalo in tropical and subtropical region, this study is important in the establishment of melatonin as the key modulator in changing concentration of casein in milk. ©2018 PVJ. All rights reservedKey words: Buffalo Casein Melatonin mTOR To Cite This Article: Riaz H, Deng TX, Ma XY, Liang SS, Duan AQ, Lu XR, Yousuf MR, Pang CY and Liang XW, 2018. Effect of melatonin on casein expression in buffalo mammary epithelial cells. Pak Vet J, 38(3): 333-336. http://dx.

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Section: Discussionsupporting

confidence: 59%

Effect of Melatonin on Casein Expression in Buffalo Mammary Epithelial Cells

Riaz¹

2018

PVJ

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“…Problems such as hyperprolactinemia (reduction of pulsatile gonadotrophin secretion) are caused by the direct effect of stress and adverse environmental factors on the neuroendocrine configuration. Similarly in cattle, the estrous cycle of the buffalo is divided into four periods, pro-estrus, estrus, metaestrus and diestrous (Ramadan, 2017). Note.…”

Section: Female Buffalomentioning

confidence: 99%

Reproductive Characteristics of Buffaloes: A Review

Pirondi¹,

Teixeira²,

Lima³

et al. 2019

JAS

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Interest in buffalo breeding has increased, due to certain advantages in relation to cattle breeding. The objective of this article was to review the reproductive biological characteristics of buffaloes. Buffaloes have their peculiarities regarding the reproductive characteristics, the females are seasonal polyestrous breeders of short days, the puberty’s buffalo is reached in ages more than in the bovine, the detection of the estrus is more difficult because of the estrus it happens at dawn and the females of buffalo have few physiological changes producers have difficulty detecting estrus. The gestation period varies between 300 and 320 days. Males have smaller external reproductive organs and less intense sexual behavior in relation to cattle. Buffalo females exhibit reproductive differences such as the hyperpigmented vulva and smaller and lighter, more rigid and tortuously inner structures with more muscle tone. There are some advantages in the creation of buffaloes were evidenced, such as their rusticity and adaptation to areas that would be unsuitableto cattle production, besides counting on products such as milk and meat of good quality. Several protocols of oestrus and ovulation synchronization have been adopted, to improve reproductive efficiency. These protocols have resulted in a pregnancy rates increase of 30% to 50%. It is concluded that buffalo breeding has space within the animal production system, especially in areas that would be suitable for the breeding of other ruminant species.

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“…These hormones are regulated by the hypothalamic-pituitary and gonadal axis (Bhimte et al, 2021). The level of circulating reproductive hormones affects the reproductive performance of cattle and buffaloes (Ramadan, 2017). The regulation and secretory pattern of these hormones are affected by different disease outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Effects of Pasturella Multocida B:2 and its immunogens (LPS and OMP) on reproductive hormones in Nili-Ravi Buffaloes

2024

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Livestock is a fundamental part of the agriculture industry in Pakistan and contributes more than 11.53% to GDP. Among livestock species, the buffaloes are regarded as the black gold of Pakistan. Being the highest milk producers globally, Nili-Ravi buffaloes are the most famous ones. Buffaloes are affected by many endemic diseases, and "Hemorrhagic septicemia" (HS) is one of them. This study was designed to ascertain the effects of experimental exposure ofP. multocida B:2 (oral) and its immunogens, i.e., LPS (oral and intravenous) and OMP (oral and subcutaneous) on reproductive hormonal profiles in Nili-Ravi buffaloes. Repeated serum samples were collected from the jugular vein of experimental animals for 21 days (0, 02, 04, 08, 12, 16, 20, 24, 36, 48, 72, 120, 168, 216, 264, 360, 456 and 504 hours). Hormonal assays to determine the serum concentrations of Gonadotropin-releasing hormone (GnRH), Follicle-stimulating hormone (FSH), Luteinizing hormone (LH), Estrogen (E2) and progesterone (P4) were performed using (MyBioSource) commercial Elisa kits. The hormonal profile of all treatment groups of the buffalo heifers exhibited significant (P<0.05) variations as compared to the control group (G-1). These results indicate suppression in Nili-Ravi buffaloes' reproductive hormonal profile on exposure to P. multocida B:2 and its immunogens. This influence warrants that exposure to H.S may be a possible reason for delayed puberty and poor reproduction performance in Nili-Ravi buffaloes.

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Role of Melatonin in Reproductive Seasonality in Buffaloes

Cited by 10 publications

References 65 publications

Effect of Melatonin on Casein Expression in Buffalo Mammary Epithelial Cells

Effect of Melatonin on Casein Expression in Buffalo Mammary Epithelial Cells

Reproductive Characteristics of Buffaloes: A Review

Effects of Pasturella Multocida B:2 and its immunogens (LPS and OMP) on reproductive hormones in Nili-Ravi Buffaloes

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