Role of Nitric Oxide in Gastric Injury Induced by Hemorrhagic Shock in Rats

Denizbaşı, Arzu; Yeğen, Cumhur; Öztürk, Melek; Yeğen, Berrak Ç.

doi:10.1159/000028389

Cited by 4 publications

(8 citation statements)

References 30 publications

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“…As the final step, the amount of the protein carbonylated was determined by a formula comparing the proportion of these two readings. Thus, the result shows us the amount of change in the structure of the protein, in nmol/mg, as a result of the oxidation of the amino acid sequence [17].…”

Section: Protein Oxidation Measurementsmentioning

confidence: 99%

“…The stomach was quickly removed and used for the macroscopical and histological evaluation of the damage. The lesion index was measured as the length of the gastric lesion observed macroscopically in millimeters [17].…”

Section: Hemorrhagic Shock-induced Gastric Damagementioning

confidence: 99%

“…The lesion index was measured as the length of the gastric lesion observed Ünlüer/Denizbaşi/Özyazgan/Akkan macroscopically in millimeters [17]. Each stomach was dissected in parallel strips perpendicular to the lesser curvature and at a distance of 2 mm.…”

Section: Histological Evaluation Of Gastric Mucosal Damagementioning

confidence: 99%

“…After 50 Ìl of homogenate sample was added to the tube containing 2,900 Ìl K 2 HPO 4 , 30 Ìl o-dianiside and 30 Ìl H 2 O 2 , the oxidation reaction was started. One unit of enzyme activity was defined as the amount of MPO present that caused a change in absorbance of 1.0/min at 655 nm and 37°C; the results were expressed as units per gram of wet weight [17].…”

Section: Tissue Myeloperoxidase (Mpo) Measurementsmentioning

confidence: 99%

“…At the end of 15 min a phenol solution was added and kept for an hour at room temperature. The change in absorbance was evaluated at 750 nm and the total protein content was calculated using linear regression of the standard curve [17].…”

Section: Protein Oxidation Measurementsmentioning

confidence: 99%

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Role of Bombesin and Cholecystokinin Receptors in Gastric Injury Induced by Hemorrhagic Shock in the Rat

et al. 2003

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Bombesin has been shown to have trophic effects on the gastrointestinal tissue. Bombesin has direct mitogenic effects besides stimulating release of gastric hormones. The aim of this study was to investigate the effect of bombesin in hemorrhagic shock-induced stress ulcers in rats, and the role of cholecystokinin (CCK) receptors in this activity. Hemorrhagic shock was created by withdrawing 3 ml blood/200 g b.w. of rats. At the end of the 1-hour hypovolemic shock period, histological analysis, gastric ulcer index, gastric myeloperoxidase activity and gastric protein oxidation levels were determined. When given before the hemorrhage, subcutaneous bombesin (10 µg/kg) reduced macroscopically gastric ulcer index (p < 0.05). Blockade of CCK-A receptors with intraperitoneal MK-329 (1 mg/kg) did not reverse bombesin-induced gastroprotection. Blockade of CCK-B receptors with intraperitoneal L-365,260 (25 mg/kg) reversed bombesin-induced gastroprotection. Blockade of the two receptors resulted in no gastroprotection at all. It is concluded that bombesin treatment attenuated hemorrhagic shock-induced stress ulcers in rats via CCK receptors.

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Section: Protein Oxidation Measurementsmentioning

confidence: 99%

Section: Hemorrhagic Shock-induced Gastric Damagementioning

confidence: 99%

Section: Histological Evaluation Of Gastric Mucosal Damagementioning

confidence: 99%

Section: Tissue Myeloperoxidase (Mpo) Measurementsmentioning

confidence: 99%

Section: Protein Oxidation Measurementsmentioning

confidence: 99%

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Role of Bombesin and Cholecystokinin Receptors in Gastric Injury Induced by Hemorrhagic Shock in the Rat

et al. 2003

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Role of Prostaglandins, Nitric oxide, Sulfhydryls and Capsaicin-sensitive Neurons in Gastroprotection of Stigmasterol and β-Sitosterol

Sánchez-Mendoza

Arrieta

Navarrete

2008

Natural Product Communications

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In this investigation the gastroprotective activity of stigmasterol and β-sitosterol was evaluated. Gastric mucosal damage was induced in rats by intragastric ethanol (1 mL/rat). Rats treated orally with stigmasterol suspended in Tween 80 at 10, 30, 100 and 300 mg kg -1 showed 26.2, 39.6, 58.3 and 70.7% gastroprotection, respectively. β-Sitosterol at 10, 30,100 and 300 mg kg -1 showed 21.6, 42.5, 48.5 and 71.2% gastroprotection, correspondingly. The gastroprotection observed at 30 mg kg -1 for stigmasterol and β-sitosterol was attenuated in rats pretreated with indomethacin, (10 mg kg -1 , s. c.), N G -nitro-L-arginine methyl ester (L-NAME, 70 mg kg -1 , i. p.) and capsaicin (125 mg kg -1 , s. c), suggesting that the gastroprotective mechanism of these sterols involves, at least in part, the participation of prostaglandins, nitric oxide (NO) and capsaicin-sensitive sensory neurons (CPSN). The gastroprotection of β-sitosterol was also attenuated by the pretreatment with N-ethylmaleimide (NEM, 10 mg kg -1 , s. c.) indicating that endogenous sulfrydryls may be involved in the gastrorpotection of this compound. Carbenoxolone was used as a gastroprotective model drug and showed a dose-dependent gastroprotective effect (25.7, 33.6 and 88.3% of gastroprotection, at 3, 10 and 30 mg kg -1 , respectively). The partial participation of PGs, sulfhydryls and NO was observed in the gastroprotective mechanism of carbenoxolone.

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Gastroprotective effect of Astragaloside IV: role of prostaglandins, sulfhydryls and nitric oxide

Navarrete

Arrieta

Terrones

et al. 2005

Journal of Pharmacy and Pharmacology

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This investigation evaluated the gastroprotective activity of Astragaloside IV, a cycloartane-type triterpene glycoside isolated from Astragalus zahlbruckneri. Gastric mucosal damage was induced in rats by intragastric ethanol (1 mL/rat). Rats treated orally with Astragaloside IV suspended in Tween 80 at 3, 10 and 30 mg kg(-1), showed 15, 37 and 52% gastroprotection, respectively. The gastroprotection observed at 30 mg kg(-1) for this compound was attenuated in rats pretreated with N(G)-nitro-L arginine methyl ester (70 mg kg(-1), i.p), a nitric oxide (NO)-synthase inhibitor, suggesting that the gastroprotective mechanism of this glycoside involves, at least in part, the participation of NO. The gastroprotective effect of Astragaloside IV was not affected by the inhibition of prostaglandin synthesis with indometacin (10 mg kg(-1), s.c.) nor by the block of endogenous sulfhydryls with N-ethylmaleimide (NEM, 10 mg kg(-1), s.c.). Carbenoxolone was used as a gastroprotective model drug and showed a dose-dependent gastroprotective effect (25, 43 and 88% of gastroprotection, at 3, 10 and 30 mg kg(-1), respectively). The partial participation of prostaglandins, sulfhydryls and NO was observed in the gastroprotective mechanism of carbenoxolone.

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Role of Nitric Oxide in Gastric Injury Induced by Hemorrhagic Shock in Rats

Cited by 4 publications

References 30 publications

Role of Bombesin and Cholecystokinin Receptors in Gastric Injury Induced by Hemorrhagic Shock in the Rat

Role of Bombesin and Cholecystokinin Receptors in Gastric Injury Induced by Hemorrhagic Shock in the Rat

Role of Prostaglandins, Nitric oxide, Sulfhydryls and Capsaicin-sensitive Neurons in Gastroprotection of Stigmasterol and β-Sitosterol

Gastroprotective effect of Astragaloside IV: role of prostaglandins, sulfhydryls and nitric oxide

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