Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa

Sommer, T.; Innocenti, Francesco Degli; Russo, Vincenzo

doi:10.1007/bf00397889

Cited by 18 publications

(9 citation statements)

References 6 publications

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“…In liquid medium, nitrogen and glucose starvation leads to conidiation (Guignard et al, 1984;Muller & Russo, 1989), and no protoperithecia. On solid agar, nitrogen limitation induces protoperithecia formation (Sommer et al, 1987) and suppresses conidiation, while glucose limitation induces conidiation and suppresses protoperithecia formation (Ricci et al, 1991). In our experiments, despite their limited number and the variation between them, it was apparent that the expression of bli-7 and am differed significantly in cultures grown in liquid and on solid medium.…”

Section: Discussionmentioning

confidence: 58%

“…Sexual as well as asexual differentiation of N. crassa is induced by nitrogen limitation (Nelson et al, 1975;Guignard et al, 1984;Sommer et al, 1987;Muller & Russo, 1989;Ricci et al, 1991). Both developmental processes are also influenced by light (Faull, 1930;Sargent et al, 1966;Sargent & Briggs, 1967 ;Siege1 et al, 1968 ;Turian, 1977;Degli-Innocenti et al, 1983 ;Sommer et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Both developmental processes are also influenced by light (Faull, 1930;Sargent et al, 1966;Sargent & Briggs, 1967 ;Siege1 et al, 1968 ;Turian, 1977;Degli-Innocenti et al, 1983 ;Sommer et al, 1987). We have analysed transcript levels of blue-light-inducible genes under conditions of nitrogen limitation.…”

Section: Discussionmentioning

confidence: 99%

“…Nitrogen limitation induces conidiation in liquid media (Guignard et al, 1984, Muller & Russo, 1989, and both inhibits conidiation (Ricci et al, 1991) and induces protoperithecia formation (Sommer et al, 1987;Ricci et al, 1991) on solid media. Since different developmental processes may be induced by the same stimulus, depending on growth conditions, we have compared the effects of nitrogen limitation on the transcription patterns of photoregulated genes in mycelia grown in liquid culture and on solid agar.…”

Section: Nitrogen Regulation Of Light-inducible Genes In Mycelia Growmentioning

confidence: 99%

“…conidiophores develop towards light (Faull, 1930;Siegel et al, 1968), and illuminated cultures produce conidia faster than dark-grown cultures (Siegel el 1968;Turian, 1977 , 1987). In darkgrown mycelia about 10 protoperithecia form per cm2; this increases to about 300 per cm2 after blue light illumination (Sommer et al, 1987). Recently, two sets of genes have been identified which are of interest with respect to the developmental programs in N. crassa.…”

Section: Introductionmentioning

confidence: 99%

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Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Sokolovsky

Lauter

Müller‐Röber

et al. 1992

Journal of General Microbiology

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Sexual and asexual differentiation of Neurospora crassa are influenced by nitrogen availability and blue light. Nitrogen Limitation induces the production of protoperithecia on solid medium and conidia in liquid medium. Both developmental processes are stimulated by blue light. We have analysed changes in mRNA levels for a variety of light-inducible genes (aZ, bli and con) under conditions of nitrogen limitation. We show that the photoregulated genes aZ-1, aZ-2, bli-4, bli-7, con-5 and con-10 are also regulated by nitrogen limitation. These genes exhibited nitrogen regulation in the nonphotoresponsive mutant strains wc-1 and wc-2. Therefore, the wc-1 and we-2 gene products, although necessary for photoregulation of these d, bli and con genes, are not required for nitrogen regulation.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Nitrogen Regulation Of Light-inducible Genes In Mycelia Growmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Sokolovsky

Lauter

Müller‐Röber

et al. 1992

Journal of General Microbiology

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Development in Neurospora crassa

Russo¹,

Pandit²

1992

Development

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The Ncypt1 gene from Neurospora crassa is located on chromosome 2: molecular cloning and structural analysis

et al. 1992

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Small GTP-binding proteins are encoded by ras-like genes and play a central role in cell differentiation and membrane vesicle transport. By screening genomic and cDNA libraries of the Ascomycete fungus Neurospora crassa with Zmypt genes from Zea mays we have isolated a member of the ypt gene family, Ncypt1. The gene resides on a 4 kb fragment of genomic DNA and contains four introns, which interrupt the coding sequence of a protein of 203 amino acid residues. The Ncytp1 gene was assigned to a single-copy gene encoding a transcript of 1.5 kb and a protein of 26,000 daltons. The gene maps on linkage group IIR between DB0001 and ccg-2 close to the Fsr-3 locus. Analysis of the nucleotide sequence and the deduced protein sequence revealed a striking homology to yeast, mouse and human genes encoding small GTP-binding proteins that are related to the ras supergene family. Homology was most significant to ypt1 from Schizosaccharomyces pombe, Mus musculus and Homo sapiens sharing 84.8%, 82.3%, and 82.3% identity, respectively. Common domains present in other small GTP-binding proteins were identified in the predicted sequence of the NCYPT1 protein, and the arrangement of peptide motifs sharing similarity with well characterized, small GTP-binding proteins suggests that the NCYPT1 protein is a GTPase. The C-terminal region extending from amino acid residues 175 to 199 shares only weak amino acid sequence similarity with other eukaryotic GTPases. Like other RAS proteins the NCYPT1 protein contains two conserved C-terminal cysteine residues, suggesting post-translational modification(s) by fatty acylation required for membrane anchoring. The high degree of homology between the NCYPT1 protein and eukaryotic YPT proteins suggests that NCYPT1 could be involved in the control of secretory processes.

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Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa

Cited by 18 publications

References 6 publications

Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Nitrogen regulation of blue light-inducible genes in Neurospora crassa

Development in Neurospora crassa

The Ncypt1 gene from Neurospora crassa is located on chromosome 2: molecular cloning and structural analysis

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