Role of Pilus Proteins in Adherence and Invasion of Streptococcus agalactiae to the Lung and Cervical Epithelial Cells

Sharma, Puja Kumari; Lata, Hem; Arya, Deepak Kumar; Kashyap, Arun K.; Kumar, Hemant; Dua, Meenakshi; Ali, Arif; Johri, Atul Kumar

doi:10.1074/jbc.m112.425728

Cited by 54 publications

(44 citation statements)

References 39 publications

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“…Several studies have previously reported that unique GBS strains differ in their abilities to attach to and invade host cells (11)(12)(13)(14)(15)(16). However, the strains tested in the prior studies were chosen based on serotype, a phenotypic characterization dictated by capsular polysaccharide genes, rather than on a genotypic characterization, such as ST.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that GBS effectively adheres to and invades epithelial and endothelial cells. Additionally, GBS strains of different serotypes differ in their abilities to associate with host cells (11)(12)(13)(14)(15)(16); however, such studies selected strains on the basis of cps type rather than ST. Because cps is horizontally transferred between strains and there is evidence of capsule switching (17,18), selecting strains based on ST, or genetic backbone, is warranted. Comparing the hypervirulent lineage, ST-17, with other lineages with respect to the ability to attach to and invade host cells will facilitate the identification of factors that play an important role in GBS disease development.…”

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confidence: 99%

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Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

et al. 2014

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

et al. 2014

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“…It has already been shown that an acidic pH can modulate the expression of a large number of proteins in GBS, including the proteins involved in surface adhesion (43,44). Several proteins have already been reported to be important for ST-17 strain adhesion to solid surfaces or attachment to host cells or extracellular matrix (21,42,45,46). Our data suggest that the contradictory reports about the effects of pH on GBS adherence to epithelial cells (44,(47)(48)(49), like previous reports about the effects of pH on biofilm formation, could be due to the limited number of GBS strains tested and the methods used.…”

Section: Figmentioning

confidence: 99%

Acidic pH Strongly Enhances In Vitro Biofilm Formation by a Subset of Hypervirulent ST-17 Streptococcus agalactiae Strains

D’Urzo

Martinelli

Pezzicoli

et al. 2014

Appl Environ Microbiol

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Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a primary colonizer of the anogenital mucosa of up to 40% of healthy women and an important cause of invasive neonatal infections worldwide. Among the 10 known capsular serotypes, GBS type III accounts for 30 to 76% of the cases of neonatal meningitis. In recent years, the ability of GBS to form biofilm attracted attention for its possible role in fitness and virulence. Here, a new in vitro biofilm formation protocol was developed to guarantee more stringent conditions, to better discriminate between strong-, low-, and non-biofilm-forming strains, and to facilitate interpretation of data. This protocol was used to screen the biofilm-forming abilities of 366 GBS clinical isolates from pregnant women and from neonatal infections of different serotypes in relation to medium composition and pH. The results identified a subset of isolates of serotypes III and V that formed strong biofilms under acidic conditions. Importantly, the best biofilm formers belonged to serotype III hypervirulent clone ST-17. Moreover, the abilities of proteinase K to strongly inhibit biofilm formation and to disaggregate mature biofilms suggested that proteins play an essential role in promoting GBS biofilm initiation and contribute to biofilm structural stability. Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a leading cause of invasive neonatal infections worldwide. It is a common colonizer of the gastrointestinal and urogenital tracts of up to 40% of healthy individuals (1). However, under certain circumstances, GBS can become a life-threatening pathogen causing invasive infections in human neonates (2, 3). Earlyonset group B streptococcal disease occurs in infants less than 7 days old, and late-onset disease (LOD) occurs in infants between 7 and 89 days old. GBS is usually transmitted from mothers to newborns during childbirth (4), but it can also penetrate the human placenta (5), and in the case of LOD, it can be nosocomially acquired.Historically, GBS isolates have been classified into 10 different serotypes according to their capsular polysaccharide composition (6, 7). Multiple surveillance studies have indicated that all serotypes are able to colonize the vagina and perianal region of pregnant women, but five serotypes (Ia, Ib, II, III, and V) are predominant and are also the most frequent in human infections (8-12). In particular, serotype III accounts for 30 to 76% of neonatal disease cases (13,14). The use of multilocus sequence typing (MLST) allowed the classification of GBS isolates independently from their capsular serotypes and the identification of the bacterial genogroups more often associated with invasive infections in newborns (15). Serotype III isolates of a particular genotype cluster, sequence type 17 (ST-17), disproportionately cause late-onset GBS disease (15-19) and more frequently cause meningitis than other STs do (20). The precise mechanism by which ST-17 causes LOD more frequently than other STs do is not well unde...

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“…Pilus proteins have been described as having a key role in S. agalactiae pathogenesis (Maisey et al, 2007;Konto-Ghiorghi et al, 2009) and have been proposed as good vaccine candidates (Sharma et al, 2013). Although the pilus proteins do not belong to the core genome of all 15 strains of S. agalactiae, promising results in development of universal vaccines have been proposed by a combination of immunogenic pilus proteins of three PIs (Margarit et al, 2009).…”

Section: Continued On Next Page Prediction Of Vaccine Targets In Strementioning

confidence: 99%

In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

Pereira

Soares²,

Blom³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Streptococcus agalactiae (Lancefield group B; group B streptococci) is a major pathogen that causes meningoencephalitis Effective vaccines against the different serotypes or genotypes of pathogenic strains from the various hosts would be useful. We used an in silico strategy to identify conserved vaccine candidates in 15 genomes of group B streptococci strains isolated from human, bovine, and fish samples. The degree of conservation, subcellular localization, and immunogenic potential of S. agalactiae proteins were investigated. We identified 36 antigenic proteins that were conserved in all 15 genomes. Among these proteins, 5 and 23 were shared only by human or fish strains, respectively. These potential vaccine targets may help develop effective vaccines that will help prevent S. agalactiae infection.

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Role of Pilus Proteins in Adherence and Invasion of Streptococcus agalactiae to the Lung and Cervical Epithelial Cells

Cited by 54 publications

References 39 publications

Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

Association and Virulence Gene Expression Vary among Serotype III Group B Streptococcus Isolates following Exposure to Decidual and Lung Epithelial Cells

Acidic pH Strongly Enhances In Vitro Biofilm Formation by a Subset of Hypervirulent ST-17 Streptococcus agalactiae Strains

In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

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