Role of RAD51AP1 in homologous recombination DNA repair and carcinogenesis

Pires, Elena; Sung, Patrick; Wiese, Claudia

doi:10.1016/j.dnarep.2017.09.008

Cited by 42 publications

(37 citation statements)

References 63 publications

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“…A former study highlighted the potential of targeting RAD51 as a new and effective therapeutic strategy for NSCLC . RAD51AP1 is a DNA‐binding protein that could stimulate the activity of RAD51 , which plays an important role in RAD51 ‐mediated homologous recombination . Existing literature has revealed that RAD51AP1 is highly expressed in ovarian and lung cancers, and silencing RAD51AP1 has an inhibitory effect on ovarian and lung cancer cell proliferation in vitro through RNA interference .…”

Section: Introductionmentioning

confidence: 99%

Silencing of RAD51AP1 suppresses epithelial–mesenchymal transition and metastasis in non‐small cell lung cancer

Wang

Qiao

et al. 2019

Thoracic Cancer

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Background Non‐small cell lung cancer (NSCLC) is a major cause of cancer‐related mortality and is frequently accompanied by metastasis. The crucial roles of genes in lung cancer have attracted attention. Thus, this study aimed to investigate the effects of RAD51AP1 on the epithelial–mesenchymal transition (EMT) and metastasis of NSCLC. Methods The positive expression rate of the RAD51AP1 protein was examined. NSCLC cells were transfected with a series of plasmids to alter the expression of RAD51AP1 to clarify the influence of RAD51AP1 on EMT and metastasis in NSCLC, as well as NSCLC cell migration, invasion, apoptosis, proliferation, and cloning. An in vivo experiment was conducted to determine the oncogenicity of human NSCLC cells in nude mice. Results RAD51AP1 was highly expressed in NSCLC tissues. Furthermore, we found promotion of N‐cadherin, vimentin, fibronectin, MMP‐2, OPN, CD62, and TMP‐2, but inhibition of E‐cadherin, occludin, cytokeratin in the context of elevated RAD51AP1 expression. An in vivo experiment also confirmed that silencing of RAD51AP1 could inhibit NSCLC tumor formation and growth. Conclusion Our results revealed that RAD51AP1 silencing suppressed the EMT and metastasis of NSCLC, thereby highlighting its potential as a promising novel target for NSCLC treatment.

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Section: Introductionmentioning

confidence: 99%

Silencing of RAD51AP1 suppresses epithelial–mesenchymal transition and metastasis in non‐small cell lung cancer

Wang

Qiao

et al. 2019

Thoracic Cancer

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“…Studies have reported that the high expression of RAD51AP1 is associated with poor prognosis in patients with breast and ovarian cancer [34]. In some tumor cells and tissues, the increased RAD51AP1 may be related to the advantages of selective growth, enabling the replication of early tumor cells and metastatic cells [35,36].…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of significant genes in esophageal squamous cell carcinoma by bioinformatical analysis

Ren

Zhang

Pan

et al. 2020

Preprint

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Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers with notably high incidence and mortality rates. However, the molecular mechanism underlying ESCC pathogenesis and prognosis is very complicated. The main objective of our investigation has been to obtain some knowledge of significant genes with poor outcome and their underlying mechanisms.Methods: Gene expression profiles of GSE26886, GSE23400, GSE20347 and GSE17351 were available from GEO database. The differentially expressed genes (DEGs) were identified, and function enrichment analyses were performed. The protein-protein interaction network (PPI) was constructed and the module analysis was performed using STRING and Cytoscape software.Results: A total of 105 DEGs were identified between normal esophagus and ESCC bioinformatical analysis samples. Functional annotations of the common DEGs indicate that extracellular matrix (ECM) remodeling plays a key role in tumor formation and progression.18 hub genes were identified and disease free survival analysis showed that CDKN3, RAD51AP1, KIF4A may be involved in poor prognosis in ESCC patients.Conclusions: DEGs and hub genes identified in the present study help us understand the molecular mechanisms underlying the carcinogenesis and progression of ESCC, and provide candidate targets for diagnosis and treatment of ESCC.

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“…When the doublestranded structure of DNA is injured, RAD51 exploits a sister DNA molecule as a template that allows for homologous recombination (HR) in the damaged region, which maintains the stability of the genes [4]. While RAD51 displays a protective role, scholars have found that RAD51 is overexpressed in several tumor types, including breast, pancreatic, head and neck, prostate, non-small cell lung, and esophageal cancers [5,6]. Recently, Yuan et al [7] discovered that high RAD51 expression was directly associated with increased chemoand radio-resistance, along with dismal outcomes and prognoses in breast cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

Sun

et al. 2020

Analytical Cellular Pathology

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Purpose. To examine the expression of RAD51 in oral squamous cell carcinoma (OSCC) and analyze its connection with pathological grade, clinical stage, and lymphatic metastasis potential. Methods. For this study, 74 OSCC samples, 15 normal mucosa tissues, and 11 normal skin tissue samples were collected. RAD51 expression was investigated using immunohistochemistry. A follow-up visit was used to assess the prognosis of each patient. We compared RAD51 expression in oral mucosa epithelial cells (OMECs), keratinocytes, and tongue squamous cell carcinoma cells (TSCCs) by Western blot analysis. Results. RAD51 expression was higher in tumor cells than in normal mucosal tissues. In addition, RAD51 expression was associated with higher tumor differentiation (P<0.05). Also, RAD51 expression was higher (P<0.05) in patients with lymphatic metastases, and relapse rates were also higher in patients with elevated RAD51 levels (P=0.052). In addition, RAD51 expression levels were highest in the skin keratinocytes, followed by the TSCCs and OMECs. Conclusion. A strong positive correlation was found between RAD51 expression and the degree of malignancy in OSCC patients, suggesting that RAD51 could be an excellent prognostic indicator for OSCC patients.

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Role of RAD51AP1 in homologous recombination DNA repair and carcinogenesis

Cited by 42 publications

References 63 publications

Silencing of RAD51AP1 suppresses epithelial–mesenchymal transition and metastasis in non‐small cell lung cancer

Silencing of RAD51AP1 suppresses epithelial–mesenchymal transition and metastasis in non‐small cell lung cancer

Screening and identification of significant genes in esophageal squamous cell carcinoma by bioinformatical analysis

Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

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