Role of TGF-β1 in Human Colorectal Cancer and Effects after Cantharidinate Intervention

Ma, Jie; Gao, Hai‐Mei; Hua, Xing; Lu, Zeyuan; Gao, Huimin

doi:10.7314/apjcp.2014.15.9.4045

Cited by 7 publications

(7 citation statements)

References 14 publications

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“…In the CRC patients included in the study group, TGFβ1 expression level in the tumor tissue was higher than in the adjacent normal tissue, although not statistically signi cant. Literature data present TGFβ1 as being related to tumor size and tumor location [25]. In our study, we observed an overexpression of this gene in the tumor samples, but this was not statistically signi cant.…”

Section: Discussioncontrasting

confidence: 67%

TIMP-1 Expression in Human Colorectal Cancer Is Associated with SMAD3 Gene Expression Levels: A Pilot Study

Braicu

Ionescu

Chiorean

et al. 2014

JGLD

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The prognosis of colorectal cancer (CRC) varies considerably, and there is a compelling need to identify novel biomarkers with prognostic significance. The aim of the present study was to evaluate the prognostic value of a panel of six genes (CDH1, SMAD3, TGFβ1, ICAM-1, TIMP-1 and MUC12) in CRC patients. METHODS. We evaluated these genes by qRT-PCR in normal and CRC tumor tissue, and correlated the relative gene expression values with clinical, pathological aspects and other biological factors. RESULTS. RNA expression levels of CDH1, SMAD3, TGFβ1, ICAM-1, TIMP-1 and MUC12 were measured by qRT-PCR in a set of 39 tumor samples and non-cancer tissue. Statistically significant increases in expression levels were found for ICAM-1 and TIMP-1 when comparing tumor samples to the non-tumor group. CONCLUSIONS. Among the genes which displayed differential expressions between tumor tissue and adjoining normal tissue, the ones that presented statistically significant correlations were TIMP-1 and SMAD3, possibly with prognostic significance.

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Section: Discussioncontrasting

confidence: 67%

TIMP-1 Expression in Human Colorectal Cancer Is Associated with SMAD3 Gene Expression Levels: A Pilot Study

Braicu

Ionescu

Chiorean

et al. 2014

JGLD

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“…Malignancies such as CRC are currently considered an important area of study, due to the burden of the disease and the mortality rate (2)(3)(4)(5). In numerous types of cancer, it has been established that aberration in genes encoding TGF-β signaling components can contribute to colon carcinogenesis in humans (7,8). This signaling pathway controls numerous cellular functions, including epithelial cell proliferation, apoptosis and migration, in addition to tumor initiation, progression and metastasis (8,9), making it a suitable target for cancer therapy (9,10).…”

Section: Discussionmentioning

confidence: 99%

“…and Smad3 components associates with Smad4, and these translocate to the nucleus to activate the transcription of downstream target genes (7)(8)(9). It is well-established that growth factors with a broad range of cellular effects, such as TGF-β, must be subject to extensive regulation to control their expression and function.…”

Section: Modulation Of Transforming Growth Factor-β Signaling Transdumentioning

confidence: 99%

“…Transforming growth factor β (TGF-β) has been identified as a key growth factor triggering varied biological processes, including proliferation, differentiation and programmed cell death (apoptosis). Studies indicate that this factor functions in signaling during the later stages of certain types of cancer and promotes tumor development, progression and metastasis (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of transforming growth factor-β signaling transducers in colon adenocarcinoma cells induced by staphylococcal enterotoxin B

Akbari

Mobini

Maghsoudi

et al. 2015

Molecular Medicine Reports

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Colorectal cancer (CRC) is a notable cause of cancer‑associated mortality worldwide, making it a pertinent topic for the study of cancer and its treatment. Staphylococcal enterotoxin B (SEB), an enterotoxin produced by Staphylococcus aureus, has been demonstrated to exert anticancer and antimetastatic effects due to its ability to modify cell immunity and cellular signaling pathways. In the current study, SEB was investigated, including whether it exerts its growth inhibitory effects on colon adenocarcinoma cells. This may occur through the manipulation of a key tumor growth factor, termed transforming growth factor‑β (TGF‑β), and its signaling pathway transducer, Smad2/3. The human colon adenocarcinoma HCT116 cell line was treated with different concentrations of SEB, and cell number was measured using MTT assay at different treatment times. Smad2/3 RNA expression level was analyzed in untreated or SEB‑treated cells using quantitative polymerase chain reaction, which indicated significant differences between cell viability and Smad2/3 expression levels. SEB effectively downregulated Smad2/3 expression in the HCT116 cells at concentrations of 1 and 2 µg/ml (P=0.0021 and P=0.0017, respectively). SEB concentrations that were effective at inhibiting Smad2/3 expression were correlated with those able to inhibit the proliferation of the cancer cells. SEB inhibited Smad2/3 expression at the mRNA level in a concentration‑ and time‑dependent manner. The present study thus proposed SEB as an agent able to significantly reduce Smad2/3 expression in colon cancer cells, provoking moderate TGF‑β growth signaling and the reduction of tumor cell proliferation.

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“…Among the multitude of different signaling molecules found in the blood, transforming growth factor-β (TGF-β) is known to aggregate metastasis by promoting epithelial-mesenchymal transition (EMT) and the invasiveness of primary carcinomas (9). TGF-β1, an ubiquitous cytokine, induces cancer cells to proliferate and promotes them to form an invasive phenotype (10,11). TGF-β ligands are secreted from cells in three isoforms (TGF-β1, TGF-β2, TGF-β3), with the latency associated protein (LAP), which makes these isoforms inactive.…”

Section: Introductionmentioning

confidence: 99%

Suppressive role of diallyl trisulfide in the activated platelet-mediated hematogenous metastasis of MDA-MB-231 human breast cancer cells

Yu-ping

Zhao

Wang

et al. 2017

International Journal of Molecular Medicine

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Accumulating evidence has indicated that garlic consumption may reduce the risk of developing several types of cancer, and extensive studies have revealed the effects of its bioactive component, diallyl trisulﬁde (DATS), on the proliferation and apoptosis of tumor cells. The present study was undertaken to examine whether DATS affects hematogenous metastasis. In view of the dynamic crosstalk interplayed by tumor cells and platelets in hematogenous metastasis, we attempted to demonstrate the role of DATS in the metastatic behavior of MDA-MB-231 human breast cancer cells, which were co-incubated with activated platelets. Indeed, our data indicated that DATS significantly blocked platelet activation and aggregation induced by platelet-activating factor (PAF), and decreased the production of thromboxane B2 (TXB2). It was also found that DATS suppressed the migration and invasion of MDA-MB-231 cells in the presence of platelets activated by PAF in vitro in a dose-dependent manner. Furthermore, our results revealed thaat the release of activated TGF-β1 in the platelet-tumor cell system was markedly attenuated by DATS. Therefore, our findings strongly suggest that the diverse pharmacological activities of DATS are at least partially reflected by the interruption of the activated platelets-mediated metastasis of breast cancer cells.

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Role of TGF-β1 in Human Colorectal Cancer and Effects after Cantharidinate Intervention

Cited by 7 publications

References 14 publications

TIMP-1 Expression in Human Colorectal Cancer Is Associated with SMAD3 Gene Expression Levels: A Pilot Study

TIMP-1 Expression in Human Colorectal Cancer Is Associated with SMAD3 Gene Expression Levels: A Pilot Study

Modulation of transforming growth factor-β signaling transducers in colon adenocarcinoma cells induced by staphylococcal enterotoxin B

Suppressive role of diallyl trisulfide in the activated platelet-mediated hematogenous metastasis of MDA-MB-231 human breast cancer cells

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