Role of the beta-lactamase of Campylobacter jejuni in resistance to beta-lactam agents

Abstract

We studied the role of the P-lactamase of Campylobacterjejuni in resistance to ,B-lactam agents. ,B-Lactamasepositive strains were more resistant than ,B-lactamase-negative strains to amoxicillin, ampicillin, and ticarcillin (P < 0.05). With penicillin G, piperacillin, imipenem, and six cephalosporins, the susceptibility levels were similar for both P-lactamase-positive and -negative strains. By using spectrophotometric and microbiological assays, the ,B-lactamase from three strains hydrolyzed ampicillin, amoxicihlin, penicillin G, cloxacillin, and, partially, cephalothin. Ticarcillin and piperacillin were partially hydrolyzed in the microbiological assay. There was no activity against five other cephalosporins or imipenem. Isoelectric focusing of the enzyme showed a pl of 8.8.Tazobactam was the best inhibitor of the enzyme, followed by clavulanic acid, sulbactam, and cefoxitin, while EDTA and p-chloromercuribenzoate had no activity. All P-lactamase-positive strains became susceptible to amoxicillin and ampicillin with 1 ,ug of clavulanic acid per ml. With the same inhibitor, there was a reduced but significant effect for ticarcillin but no effect for penicillin G or piperacillin. Sulbactam had no effect and tazobactam was effective only at 2 ,ig/ml on amoxicillin and ampicillin. The 1-lactamase of C. jejuni seems to be a penicillinase with a role in resistance for only amoxicillin, ampicillin, and ticarcillin.

Antimicrobial resistance of thermophilic Campylobacter

“…However, in some bacteria, changes in the penicillin-bindingproteins or lack of penetration of the drug into the bacteria are the main mechanisms of resistance. A large proportion of C. coli and C. jejuni produce beta-lactamases [29,30,50,62]. However, the β-lactamase of C. jejuni/coli seems to play a role only in resistance to amoxicillin, ampicillin and ticarcillin.…”

“…However, the β-lactamase of C. jejuni/coli seems to play a role only in resistance to amoxicillin, ampicillin and ticarcillin. With penicillin G, piperacillin and cephalosporins, the mechanism of resistance in C. jejuni/coli is primarily considered to be dependent on their limited ability to bind to penicillin-binding proteins and their low permeability [29,62,68].…”

Susceptibilidad antimicrobiana de Campylobacter jejuni aislado de coprocultivos en Santiago de Chile

“…En cambio, nuestras cepas presentaron 6,8% de resistencia a ampicilina, cifra similar a la descrita por Fernández y cols 18 . Campylobacter es intrínsecamente resistente a penicilina G y cefalosporinas de primera generación debido a la débil unión de estos antimicrobianos con las proteínas de unión a penicilina (PBPs) 31 . Además, se ha descrito la adquisición de β-lactamasas como una causa importante de resistencia a β-lactámicos en esta bacteria [31][32][33] .…”

“…Campylobacter es intrínsecamente resistente a penicilina G y cefalosporinas de primera generación debido a la débil unión de estos antimicrobianos con las proteínas de unión a penicilina (PBPs) 31 . Además, se ha descrito la adquisición de β-lactamasas como una causa importante de resistencia a β-lactámicos en esta bacteria [31][32][33] . En un estudio realizado por Tajada y cols, se determinó que de 100 cepas de C. jejuni y Campylobacter coli, 88% presentaban β-lactamasas y que, pese a la existencia de éstas, 77% de las cepas que las poseían eran susceptibles a amoxicilina 34 .…”

Influência da vida séssil e planctônica no perfil de resistência antimicrobiana de Campylobacter jejuni isoladas de carcaças de frangos comercializadas no Brasil

“…Campylobacter: a inativação enzimática por β-lactamases; absorção reduzida devido a alterações ou mutações nas porinas da membrana externa; bomba de efluxo e modificações estruturais das proteínas ligadoras de penicilina (PLP) (LACHANCE et al, 1991;BRASIL, 2007).…”