Rooibos flavonoids, orientin and luteolin, stimulate mineralization in human osteoblasts through the Wnt pathway

Nash, Leslie A.; Sullivan, Philip; Peters, Sandra J.; Ward, Wendy E.

doi:10.1002/mnfr.201400592

Cited by 61 publications

(49 citation statements)

References 62 publications

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“…Our data demonstrated that osteoblastic cells treatment with apigenin and luteolin at a concentration of 1 μ M significantly increased the transcriptional level of COL-I, OPN, and OCN (Figure 9(a)). Similar concentrations of these two compounds have been used, and our findings are in agreement with the other experiments [26–28]. In a previous study, we reported that linarin extracted from FCI enhanced the osteoblast differentiation in MC3T3-E1 cells in a dose-dependent manner [11].…”

Section: Resultssupporting

confidence: 92%

Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Lin

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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To understand the material basis and underlying molecular machinery of antiosteoporosis activity of the Flos Chrysanthemi Indici (FCI), the consequences of ethanol extract on the bone loss in mice induced due to ovariectomy (OVX) was evaluated. Also, the antiosteoporosis fraction obtained from the FCI ethanol extract was isolated and purified using a preparative high-speed countercurrent chromatography (HSCCC). The in vitro impact of the compounds was investigated on osteoblast proliferation and differentiation. The results revealed that ethyl acetate fraction with robust in vivo antiosteoporosis activity was obtained. The important compounds purified by HSCCC using gradient elution system included acacetin, apigenin, luteolin, and linarin. The four compounds enhanced the differentiation and proliferation of osteoblasts in MC3T3-E1 cells. They also augmented the mRNA levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COL I). The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds. The present study demonstrated that the antiosteoporosis effects of FCI did not depend on a single component, and HSCCC efficiently isolated and purified the antiosteoporosis bioactive compounds from FCI.

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Section: Resultssupporting

confidence: 92%

Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Lin

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…Flavonoids are a large class of phyto-chemicals that are widely distributed in plant foods [14–17]. Flavonoids have been found to decrease urinary excretion of calcium and phosphate, increase osteoblast activity, decrease osteoclast activity, and protect against the loss of trabecular thickness [18–20]. Previous studies showed that different plant-derived flavonoid compounds can stimulate osteoblasts function, and inhibit osteoclasts functions either alone or in combination.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Tridax procumbens flavonoids promote osteoblast differentiation and bone formation

et al. 2015

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BackgroundTridax procumbens flavonoids (TPFs) are well known for their medicinal properties among local natives. Besides traditionally used for dropsy, anemia, arthritis, gout, asthma, ulcer, piles, and urinary problems, it is also used in treating gastric problems, body pain, and rheumatic pains of joints. TPFs have been reported to increase osteogenic functioning in mesenchymal stem cells. Our previous study showed that TPFs were significantly suppressed the RANKL-induced differentiation of osteoclasts and bone resorption. However, the effects of TPFs to promote osteoblasts differentiation and bone formation remain unclear. TPFs were isolated from Tridax procumbens and investigated for their effects on osteoblasts differentiation and bone formation by using primary mouse calvarial osteoblasts.ResultsTPFs promoted osteoblast differentiation in a dose-dependent manner demonstrated by up-regulation of alkaline phosphatase and osteocalcin. TPFs also upregulated osteoblast differentiation related genes, including osteocalcin, osterix, and Runx2 in primary osteoblasts. TPFs treated primary osteoblast cells showed significant upregulation of bone morphogenetic proteins (BMPs) including Bmp-2, Bmp-4, and Bmp-7. Addition of noggin, a BMP specific-antagonist, inhibited TPFs induced upregulation of the osteocalcin, osterix, and Runx2.ConclusionOur findings point towards the induction of osteoblast differentiation by TPFs and suggested that TPFs could be a potential anabolic agent to treat patients with bone loss-associated diseases such as osteoporosis.

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“…First, in vitro co-culture studies with plasma cells and osteoblasts are limited by the lack of relevant osteoblast cell lines. The human bone marrow stroma cell lines HS-5 and HS-27 do not differentiate into osteoblasts, and other cell lines that do mineralize, such as Saos2 [41] and MG-63 [42], are actually osteosarcoma rather than osteoblast cell lines. Certain cell lines, such as the human fetal osteoblastic cell line hFOB1.19[43], which proliferate at 33.4°C and differentiate at 39.4°C or in osteogenic medium, have been explored in multiple myeloma in vitro cultures [44] and could be exploited further.…”

Section: Effects Of Osteoblasts On Multiple Myelomamentioning

confidence: 99%

Dynamic interplay between bone and multiple myeloma: Emerging roles of the osteoblast

Reagan

Liaw

Rosen

et al. 2015

Bone

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Multiple myeloma is a B-cell malignancy characterized by the unrelenting proliferation of plasma cells. Multiple myeloma causes osteolytic lesions and fractures that do not heal due to decreased osteoblastic and increased osteoclastic activity. However, the exact relationship between osteoblasts and myeloma cells remains elusive. Understanding the interactions between these dynamic bone-forming cells and myeloma cells is crucial to understanding how osteolytic lesions form and persist, and how tumors grow within the bone marrow. This review provides a comprehensive overview of basic and translational research focused on the role of osteoblasts in multiple myeloma progression and their relationship to osteolytic lesions. Importantly, current challenges for in vitro studies exploring direct osteoblastic effects on myeloma cells, and gaps in understanding the role of the osteoblast in myeloma progression are delineated. Finally, successes and challenges in myeloma treatment with osteoanabolic therapy (i.e. any treatment that induces increased osteoblastic number or activity) are enumerated. Our goal is to illuminate novel mechanisms by which osteoblasts may contribute to multiple myeloma disease progression and osteolysis to better direct research efforts. Ultimately, we hope this may provide a roadmap for new approaches to the pathogenesis and treatment of multiple myeloma with a particular focus on the osteoblast.

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Rooibos flavonoids, orientin and luteolin, stimulate mineralization in human osteoblasts through the Wnt pathway

Abstract: Orientin and luteolin, flavonoids in rooibos tea, enhance mineral content in Saos2 cells. These findings provide guidance for doses to be studied in well-established animal models.

Cited by 61 publications

References 62 publications

Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

RETRACTED ARTICLE: Tridax procumbens flavonoids promote osteoblast differentiation and bone formation

Dynamic interplay between bone and multiple myeloma: Emerging roles of the osteoblast

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