Rooting of Cultured Rose Shoots

Alderson, P. G.; McKinless, J.; Rice, R.D.

doi:10.17660/actahortic.1988.226.19

Cited by 7 publications

(2 citation statements)

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“…The maximum root count for 'John Franklin' at low to medium IBA levels was in agreement with the findings of Alderson et al (1988). Because the highest IBA and NAA levels significantly decreased root counts for 'John Franklin', 'John Paul II', and 'Landora', these concentrations should be avoided.…”

Section: Methodssupporting

confidence: 85%

Auxins, Salt Concentrations, and Their Interactions during in Vitro Rooting of Winter-hardy and Hybrid Tea Roses

Arnold¹,

Binns²,

Cloutier³

et al. 1995

HortSci

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Auxins, medium salt concentrations, and their interactive effects on rooting of two winter-hardy roses (Rosa kordesii Wulff `John Franklin' and `Champlain') and two hybrid teas (Rosa hybrida `John Paul II' and `Landora') were studied. The auxins (in mg·liter–1) IAA (0, 0.3, 1.5, 3.0, 6.0, or 15.0), IBA (0, 0.1, 0.2, 0.5, 1.0, or 3.0), and NAA (0, 0.1, 0.2, 0.5, 1.0, or 3.0) each were combined factorially with modified Murashige and Skoog (MS) medium (1/4, 1/2, 3/4, and full MS concentrations) and were tested for optimal rooting response. `John Franklin', `John Paul II', and `Landora' rooted well with low or no auxin and medium to high salt concentrations. Optimum rooting for `Champlain' was achieved with high IAA and low salts or with intermediate IBA and NAA concentrations and low to medium salts. The interactive effects of auxins and medium salts for `Champlain' showed that as salt concentration increased, the amount of IBA or NAA required for optimal rooting also increased. The effects of auxins and medium concentrations on root counts per shoot were similar to those for percent rooting. Adding auxin to the medium reduced root length for all cultivars, but salt concentration had a minimal effect. Roots generally were shortest at the highest IBA and NAA concentrations. Salt concentration had little effect on root length. Chemical names used: 1H-indole-3-acetic acid (IAA); 4-(3-indolyl)-butyric acid (IBA); α-naphthaleneacetic acid (NAA).

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Section: Methodssupporting

confidence: 85%

Auxins, Salt Concentrations, and Their Interactions during in Vitro Rooting of Winter-hardy and Hybrid Tea Roses

Arnold¹,

Binns²,

Cloutier³

et al. 1995

HortSci

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“…Also, these plantlets had reduced wilting and increased transpiration rates compared to plantlets that had been rooted in agar. Alderson and McKinless (1988) reported that rose shoots rooted in cellulose plugs developed root hairs, while those rooted in Fig. 3.…”

Section: Resultsmentioning

confidence: 97%

A simplified protocol for micropropagation of guayule (Parthenium argentatum gray)

Castillón

Cornish

2000

In Vitro Cell.Dev.Biol.-Plant

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A simple, efficient protocol for in vitro micropropagation of guayule is reported. Shoot cultures were maintained on MS (Murashige and Skoog, 1962) medium supplemented with 1.0 mg l Ϫ1 (4.4 mM) 6-benzylaminopurine and 0.025 mg l Ϫ1 (0.13 mM) a-naphthaleneacetic acid. Excised shoots were treated for 14-18 h with 100 mg l Ϫ1 (492.1 mM) indole-3-butyric acid in 0X5 Â MS salts to induce rooting. The shoots were subsequently inserted into cellulose plugs which were packed in sterile, ventilated plastic culture vessels and moistened with 0X5 Â MS medium without growth regulators. Use of cellulose plugs, liquid medium and ventilated culture vessels facilitated acclimation. Rooted shoots were transplanted into potting medium and acclimated to greenhouse conditions by covering with a cloche for 2 d, followed by daily watering for the first week.

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