ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation

Li, Xuan; Xu, Ke; Tao, Bailong; Li, Ke; Lin, Changjian; Hu, Jingwei; Wu, Jing; Wu, Yuanfeng; Liu, Shaopeng; Liu, Peng; Wang, Huaiyu; Cai, Kaiyong

doi:10.1016/j.biomaterials.2022.121683

Cited by 57 publications

(40 citation statements)

References 47 publications

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“…At the injured bone site, ROS (a pleiotropic end-product derived from cellular enzymatic reactions) play a significant role in regulating physiological processes involved in inflammatory signaling. Accumulating clinical evidence and animal research have identified that excessive ROS produced by macrophages cause oxidative stress and trigger sustained cell/tissue injury, thereby aggravating local inflammation and disrupting bone cell differentiation and osteogenesis . Hence, the fabrication of advanced biomaterials with antioxidant capacity to remove excessive ROS and protect cells from this is a key strategy in treating bone defects.…”

Section: Resultsmentioning

confidence: 99%

“…Accumulating clinical evidence and animal research have identified that excessive ROS produced by macrophages cause oxidative stress and trigger sustained cell/tissue injury, thereby aggravating local inflammation and disrupting bone cell differentiation and osteogenesis. 58 Hence, the fabrication of advanced biomaterials with antioxidant capacity to remove excessive ROS and protect cells from this is a key strategy in treating bone defects. To verify whether the piezoelectric periosteum was capable of scavenging free radicals, we performed a DPPH scavenging assay and demonstrated that PHA displayed slightly higher DPPH scavenging activity than PHBV (Figure S19A).…”

Section: Effects Of the Piezoelectric Periosteum On Ros Scavenging Pe...mentioning

confidence: 99%

“…It was previously demonstrated that an excessive or prolonged inflammatory response during bone repair tends to delay healing, thus causing nonunions. 58 Moreover, the transition of the macrophage phenotype during inflammation directly impacts subsequent complex cascades of events and determines implant success. 29 Accordingly, macrophage polarization within the whole defect region was assessed using immunofluorescence and immunohistochemical staining at 2 weeks.…”

Section: 7mentioning

confidence: 99%

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Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Liu

Shi

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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Ideal periosteum materials are required to participate in a sequence of bone repair-related physiological events, including the initial immune response, endogenous stem cell recruitment, angiogenesis, and osteogenesis. However, conventional tissue-engineered periosteal materials have difficulty achieving these functions by simply mimicking the periosteum via structural design or by loading exogenous stem cells, cytokines, or growth factors. Herein, we present a novel biomimetic periosteum preparation strategy to comprehensively enhance the bone regeneration effect using functionalized piezoelectric materials. The resulting biomimetic periosteum possessing an excellent piezoelectric effect and improved physicochemical properties was prepared using a biocompatible and biodegradable poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) polymer matrix, antioxidized polydopamine-modified hydroxyapatite (PHA), and barium titanate (PBT), which were further incorporated into the polymer matrix to fabricate a multifunctional piezoelectric periosteum by a simple one-step spin-coating method. The addition of PHA and PBT dramatically enhanced the physicochemical properties and biological functions of the piezoelectric periosteum, resulting in improved surface hydrophilicity and roughness, enhanced mechanical performance, tunable degradation behavior, and stable and desired endogenous electrical stimulations, which is conducive to accelerating bone regeneration. Benefiting from endogenous piezoelectric stimulation and bioactive components, the as-fabricated biomimetic periosteum demonstrated favorable biocompatibility, osteogenic activity, and immunomodulatory functions in vitro, which not only promoted adhesion, proliferation, and spreading as well as osteogenesis of mesenchymal stem cells (MSCs) but also effectively induced M2 macrophage polarization, thereby suppressing reactive oxygen species (ROS)-induced inflammatory reactions. Through in vivo experiments, the biomimetic periosteum with endogenous piezoelectric stimulation synergistically accelerated the formation of new bone in a rat critical-sized cranial defect model. The whole defect was almost completely covered by new bone at 8 weeks post treatment, with a thickness close to that of the host bone. Collectively, with its favorable immunomodulatory and osteogenic properties, the biomimetic periosteum developed here represents a novel method to rapidly regenerate bone tissue using piezoelectric stimulation.

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Section: Resultsmentioning

confidence: 99%

Section: Effects Of the Piezoelectric Periosteum On Ros Scavenging Pe...mentioning

confidence: 99%

Section: 7mentioning

confidence: 99%

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Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Liu

Shi

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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“…The hydrogel promoted wound healing by reducing ROS levels and upregulating M2 phenotype macrophages. Li et al 108 reported the design of a multifunctional ROS‐responsive hydrogel coating tightly adhered to the Tβ4‐loaded titanium implants to promote vascular reconstruction and bone formation by immunomodulation. Zheng et al 109 designed a ROS‐responsive PAMB‐G‐TK/4‐arm‐PEG‐SG hydrogel for localized drug‐loaded liposome delivery to consume the overproduced pathological ROS for improved cardiomyocytes activity and enhanced myocardial infarction treatment.…”

Section: Smart‐responsive Hydrogelsmentioning

confidence: 99%

Recent advances in smart‐responsive hydrogels for tissue repairing

Yang

Wang

2022

MedComm – Biomaterials and Applications

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The rapid development of biomedical materials and tissue engineering technology has played an increasingly important role in the process of tissue repair in recent years. Smart-responsive hydrogels are three-dimensional network structures formed by cross-linking of hydrophilic polymers. In addition to having conventional hydrogels that approximate the natural extracellular matrix structure and serve as delivery vehicles for functional molecules (drugs and proteins). More importantly, smart-responsive hydrogels can achieve relevant changes in material morphology or properties under the conditions of changes in physical, chemical, and biological factors, thereby achieving controlled functional molecules release. It is more urgent to design and build smart-responsive hydrogels to achieve precise tissue repair with the introduction of the concept of precision medicine and drug delivery. In this review, we highlight different types of smart-responsive hydrogels and their mechanisms of response to different stimuli and discuss their potential for application in different types of tissue repair, such as chronic wound repair, damaged heart tissue repair, brain nerve tissue repair, and other fields. Finally, we present the prospects of smart-responsive hydrogels in tissue repair. In general, the current progress in the application of smart-responsive hydrogels in tissue repair lays the foundation for future applications in other diseases.

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“…8,9 Therefore, the surface modification of 3D-printed Ti6Al4V implants has recently become an emerging focus. 10 Biogenic growth factors, such as bone morphogenetic protein-2, 11 type-I collagen, 12 and functional hydrogel coating, 13 have been introduced to promote bone ingrowth and osseointegration; however, they have certain disadvantages such as high cost, complicated manufacturing process, unstable quality, and difficult to preserve nature. 8 Mineral coating, for example Ca/P by microarc oxidation, 14 was also developed on 3D-printed Ti6Al4V porous implants with lower cost and stable quality, while its promotion of bone ingrowth was far from satisfactory, significantly hampering their clinical applications.…”

Section: Introductionmentioning

confidence: 99%

A pH-neutral bioactive glass coated 3D-printed porous Ti6Al4V scaffold with enhanced osseointegration

Wang

Guo

et al. 2023

J. Mater. Chem. B

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show abstract

ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation

Cited by 57 publications

References 47 publications

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Bioinspired Piezoelectric Periosteum to Augment Bone Regeneration via Synergistic Immunomodulation and Osteogenesis

Recent advances in smart‐responsive hydrogels for tissue repairing

A pH-neutral bioactive glass coated 3D-printed porous Ti6Al4V scaffold with enhanced osseointegration

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