Rotary culture promotes the proliferation of MCF-7 cells encapsulated in three-dimensional collagen–alginate hydrogels via activation of the ERK1/2-MAPK pathway

Abstract

Rotary cell culture systems (RCCS) have been shown to be promising for promoting three-dimensional (3D) cell growth and assembly of cells into functional tissues. In this study, 3D tissue-like spheroids of MCF-7 cells were constructed by encapsulating the cells in the collagen-alginate hydrogel, and then cultured in a RCCS to investigate the proliferation of MCF-7 cells. The results from the MTT assay showed that the proliferation rate of MCF-7 cells cultured in the RCCS was higher than that of the static culture control group, and the results from the flow cytometry revealed that the cells in S and G2/M phase were significantly increased compared to the control group. The expression of cell proliferation antigen PCNA and cyclin D1 was also examined with the results further supporting the enhanced proliferation of MCF-7 cells by the RCCS. The results from indirect immunofluorescence revealed that the rotary culture altered neither the cytoskeleton distribution nor the assembly of mitotic spindle. By examination, it was also shown that the rotary culture induced the ERK1/2-MAPK pathway. Taken together, this study demonstrated that the rotary culture could promote the proliferation of MCF-7 cells by inducing the ERK1/2 pathway.

Effect of dynamic three‐dimensional culture on osteogenic potential of human periodontal ligament‐derived mesenchymal stem cells entrapped in alginate microbeads

“…Much evidence supports the notion that culture vessel rotation in the dynamic bioreactor reduces the occurrence of sedimentation and maintains the cells suspended in a relatively stable “free fall” condition that circumvents the harmful effects associated to shear stress . In addition, the presence of a gas exchange membrane in the culture chamber favors the optimal oxygenation of thick cell‐based constructs or microbeads encapsulated cells, preventing the formation of central core necrosis . For what concerns the encapsulation of the cells into microbeads, it is important to underline that encapsulation technology may contribute to the success of cell transplantation allowing a more efficient and functional integration of cells in tissue regenerative approaches .…”

Alginate-Based Biomaterials for Regenerative Medicine Applications

“…Therefore, alginate has been blended with collagen or gelatin to enhance cell ligand-specific binding properties to fabricate hybrid scaffolds, which showed better properties for supporting cells [42,81,83,98,99]. In a recent report, other efforts were made to enhance the biological properties of alginate porous scaffolds.…”

Mussel-inspired alginate gel promoting the osteogenic differentiation of mesenchymal stem cells and anti-infection

“…However, alginate has low cell adhesiveness due to its poor protein adsorption for the hydrophilic nature [22]. Therefore, alginate has been blended with chitosan, collagen or gelatin to enhance cell interaction and has showed better properties for cell adhesion and proliferation [23][24][25][26]. Other strategies have also been carried out to enhance cell anchorage and interaction within alginate gel.…”