Ruminal Microbial Populations and Fermentation Characteristics in Beef Cattle Grazing Tropical Forage in Dry Season and Supplemented with Different Protein Levels

Silva-Marques, Renata Pereira da; Zervoudakis, Joanis Tilemahos; Nakazato, Luciano; Hatamoto-Zervoudakis, Luciana Keiko; Cabral, Luciano da Silva; Matos, Núbia Bezerra do Nascimento; Silva, Maria Isabel Leite da; Feliciano, Andresa L

doi:10.1007/s00284-019-01631-w

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…Supplementing concentrate feed and/or rumen-protected Lys and Met had no effect on the alpha diversity of the rumen bacterial community, which was in agreement with Anderson et al’s study on steers [ 29 ]. However, yaks with supplementary concentrate feed had the lowest ACE value, which was also reported in cattle grazing tropical pasture and supplemented with concentrate [ 13 ]. In a previous study on yaks, ruminal bacterial diversity increased with an increase in forage intake because of the complexity of dietary ingredients [ 4 ].…”

Section: Discussionmentioning

confidence: 89%

“…Supplementary protein fed to pregnant heifers enhanced bacterial populations involved in hemicellulose and pectin degradation and in ammonia assimilation [ 11 ]. Abundances of the genera in Firmicutes decreased in Holstein heifers when supplemented with lysine (Lys) and methionine (Met) [ 12 ], while abundances of Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, and Methanogen archaea increased in beef cattle when supplemented with concentrate feed [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Rumen Bacterial Community of Grazing Lactating Yaks (Poephagus grunniens) Supplemented with Concentrate Feed and/or Rumen-Protected Lysine and Methionine

Liu

Jiang

Hao

et al. 2021

Animals

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Traditionally, yaks graze only natural pasture all year round without supplements. Forage intake of lactating yaks is below energy and protein requirements, even in the summer, and suckling yaks lose a substantial amount of significant body weight. Today, to mitigate the loss in body weight, supplementary feed is being offered to lactating yaks. However, the effects of supplementary feed on ruminal bacterial communities in lactating yaks is unknown. In the current study, we examined the effect of supplementary feed on ruminal microbiota, using 16S rRNA sequencing, and on volatile fatty acids (VFAs). Twenty-four lactating yaks of similar body weight (218 ± 19.5 kg) and grazing natural pasture were divided randomly into four groups and received different supplements: (1) rumen-protected amino acids (RPA); (2) concentrate feed (C); (3) RPA plus C (RPA+C); and (4) no supplements (control-CON). The concentrations of total VFAs, acetate, and butyrate were greater (p < 0.05) when supplemented with concentrate feed (C and RPA+C) than without concentrate feed (CON and RPA). Bacteroidetes (B) and Firmicutes (F) were the dominant ruminal bacterial phyla in all groups. The ratio of relative abundance of F:B in RPA+C was greater than in the RPA group, while there was no difference between CON and RPC (interaction, p = 0.026). At the genus level, the relative abundances of Absconditabacteriales_SR1, Bacteroidales-RF16-group, Bacteroidales_BS11_gut_group, Prevotellaceae, and Rikenellaceae_RC9_gut_group were lesser (p < 0.05) with supplementary concentrate feed (C and RPA+C) than without concentrate feed (CON and RPA), whereas Butyrivibrio_2 and Pseudobutyrivibrio were greater (p < 0.05) with supplementary rumen-protected amino acids (RPA and RPA+C) than without rumen-protected amino acids (CON and C). These results demonstrate that supplementary feed: (1) alters the composition of rumen microbiota and concentrations of ruminal VFAs in lactating yaks; and (2) can be used to manipulate the composition of rumen microbiota.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Rumen Bacterial Community of Grazing Lactating Yaks (Poephagus grunniens) Supplemented with Concentrate Feed and/or Rumen-Protected Lysine and Methionine

Liu

Jiang

Hao

et al. 2021

Animals

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“…A study conducted by Da Silva-Marques et al [ 53 ] found that protein supplementation improves some non-harmful fermentation parameters in the rumen microbial populations of cattle destined for meat. They found a decrease in colonization by Butyrivibrio fibrisolvens and an increase in the population of Fibrobacter succinogenes , Ruminococcus albus , Ruminococcus flavefaciens , and Methanogen archaea , demonstrating that bacteria with possible probiotic activity and cellulite activity improve the quality of the meat product.…”

Section: Different Classes Of Probiotic Productsmentioning

confidence: 99%

Functional Foods, Nutraceuticals and Probiotics: A Focus on Human Health

et al. 2022

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Functional foods are classified as traditional or staple foods that provide an essential nutritional level and share potentially positive effects on host health, including the reduction of disease by optimizing the immune system’s ability to prevent and control infections by pathogens, as well as pathologies that cause functional alterations in the host. This chapter reviews the most recent research and advances in this area and discusses some perspectives on what the future holds in this area.

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“…The rumen is the site of microbial lipid metabolism [8]. Many researchers have found that increasing dietary concentrate levels improves ruminal fermentation and short-chain fatty acid (FA) production by stimulating ruminal bacterial growth [9,10]. Manipulation of the diet by increasing the proportion of concentrate could consequently provide more VFAs for the rumen microbial population in pasture-grazed animals [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of Concentrate Supplementation on the Organic Matter Intake, Rumen Fermentation and Bacterial Community of Grazing Simmental Heifers

Chen

Wang

Simujide

et al. 2021

Preprint

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Background: In grazing systems, supplementation is an effective way to improve ruminant performance. Little is known regarding how concentrate supplementation affects rumen fermentation and the bacterial community of grazing animals in the grasslands of northern China. Therefore, the objective of this study was to examine the effects of concentrate supplementation on the organic matter intake, rumen fermentation and bacterial community of grazing Simmental heifers. Twenty-four 7-month-old heifers were randomly divided into a supplement group and a control group (n = 12 per group). The control group heifers were grazed on a Leymus chinensis-based pasture without any supplementation (CON), while those in the supplement group (SUP) were grazed on the same grassland but received a concentrate supplement.Results: Compared with the CON heifers, those in the SUP group had a greater (P < 0.05) total organic matter (OM) intake and digestibility. However, heifers from the CON group had a higher (P < 0.05) herbage OM intake than those from the SUP group. The total VFA and NH3-N concentrations were significantly higher in the SUP group than in the CON group; of these, propionate, butyrate, and isobutyrate were also significantly increased in the SUP group. Furthermore, the acetate/propionate ratio was significantly decreased in the SUP group. Compared with the CON heifers, those in the SUP group had a higher bacterial richness. Firmicutes and Bacteroidetes were the two predominant bacteria at the phylum level, representing 54.32% and 38.59% of all sequences, respectively. Compared with the CON animals, the SUP animals showed significant (p < 0.05) increases in the relative abundance of Bacteroidetes and Verrucomicrobia, but supplementation did not affect the relative abundance of other bacteria at the phylum level. At the genus level, different diet treatments had an important effect on the relative abundance of the major genera (Fig. 3C). The relative abundance of the genus Butyrivibrio was greater (p < 0.05) in the SUP group than in the CON group. The relative abundances of the genera Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-010 and Ruminococcaceae_UCG-011 were higher (p < 0.05) in the CON group than in the SUP group.Conclusions: In conclusion, providing 1.28 kg of supplement (DM basis) is a nutritional management strategy that can be adopted to improve the rumen fermentation, organic matter intake and digestibility of grazing heifers. Furthermore, supplementation has significant effects on the microbial community in the rumen of heifers, which might contribute to the anatomic development of the rumen, and these findings will contribute to the direction of future research in grazing heifers.

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Ruminal Microbial Populations and Fermentation Characteristics in Beef Cattle Grazing Tropical Forage in Dry Season and Supplemented with Different Protein Levels

Cited by 10 publications

References 32 publications

Rumen Bacterial Community of Grazing Lactating Yaks (Poephagus grunniens) Supplemented with Concentrate Feed and/or Rumen-Protected Lysine and Methionine

Rumen Bacterial Community of Grazing Lactating Yaks (Poephagus grunniens) Supplemented with Concentrate Feed and/or Rumen-Protected Lysine and Methionine

Functional Foods, Nutraceuticals and Probiotics: A Focus on Human Health

Effects of Concentrate Supplementation on the Organic Matter Intake, Rumen Fermentation and Bacterial Community of Grazing Simmental Heifers

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