S-100β has a neuronal localisation in the rat hindbrain revealed by an antigen retrieval method

Yang, Qiner; Hamberger, Anders; Hydén, Holger; Wang, Shu; Stigbrand, Torgny; Haglid, Kenneth G.

doi:10.1016/0006-8993(95)00755-f

Cited by 78 publications

(35 citation statements)

References 54 publications

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“…Based on our results using both Zamboni's and paraformaldehyde fixatives, we conclude that this discrepancy most likely results from the use of different fixatives rather than from species differences or differences in the epitopes recognized by the different polyclonal CuZnSOD antibodies used in the two studies. Other studies have also demonstrated that immunoreactivity for neuronal antigens can be decreased by formaldehyde; formalin fixation abolished immunostaining for MnSOD in murine tissues (Oberley et al, 1993), and fixation with 4% paraformaldehyde decreased or abolished neuronal staining for neurofilament proteins and spectrin (Riederer, 1989) and S-100b (Yang et al, 1995) in the rat brain.…”

Section: Discussionmentioning

confidence: 93%

Differential expression of manganese and copper-zinc superoxide dismutases in the olfactory and vomeronasal receptor neurons of rats during ontogeny

Kulkarni-Narla

Getchell

1997

J. Comp. Neurol.

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Superoxide dismutases (SODs) protect cells from damage by oxygen free radicals. Manganese (Mn) SOD is preferentially induced in terminally differentiating cells; induction of copper-zinc (CuZn) SOD is more closely associated with postnatal exposure to environmental sources of oxygen free radicals. The purpose of this study was to investigate ontogenetic changes in immunoreactivity for MnSOD and CuZnSOD relative to the expression of markers of neuronal and chemosensory differentiation in olfactory and vomeronasal receptor neurons (ORNs and VRNs, respectively), which mature with different time courses. Immunoreactivity for both SODs was detected in rat ORNs at embryonic day (E) 14, the earliest time point investigated, but not until E16 in vomeronasal neuroblasts. ORNs also expressed the neuronal marker protein gene product (PGP) 9.5 and the chemosensory cell marker olfactory marker protein (OMP) at E14; vomeronasal neuroblasts expressed PGP 9.5 at E16 but were not immunoreactive for OMP until postnatal day (P) 2. Immunoreactivity for MnSOD in ORNs and VRNs generally increased pre- and postnatally to a maximum at P11. Immunoreactivity for CuZnSOD did not increase markedly until after birth, reaching maximal levels at P11-P24. Within ORNs and VRNs, the most intense immunoreactivity was localized in the dendritic and supranuclear regions. The results indicate that in ORNs and VRNs, increases in MnSOD immunoreactivity during ontogeny parallel the ongoing differentiation and maturation of chemosensory receptor neurons; in contrast, the induction of immunoreactivity for CuZnSOD is associated with postnatal exposure to the ambient oxygen and xenobiotic environment.

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Section: Discussionmentioning

confidence: 93%

Differential expression of manganese and copper-zinc superoxide dismutases in the olfactory and vomeronasal receptor neurons of rats during ontogeny

Kulkarni-Narla

Getchell

1997

J. Comp. Neurol.

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“…All the first data in vertebrates argues that the localization of S100 protein immunoreactivity is restricted to the glial cells (Legrand et al, 1981), following studies demonstrated the presence of S100 immunoreactivity in central neurons of different vertebrates (turtle, frog, fish, rabbit, cat, rat, human: Goto et al, 1988;Haglid et al, 1976;Isobe et al, 1984;Tabuchi et al, 1976). Most of these studies described the distribution of S100-immunopositive neuronal population only in limited regions, but some authors have detailed the distribution of S100-positive neurons in the whole mammalian brain with immunocytochemistry and nonradioactive in situ hybridization (Rickmann and Wolff, 1995;Yang et al, 1995a). So, this particularity of the cerebellum of the adult zebrafish must be investigated in other fishes and its significance if any remains to be demonstrated.…”

Section: Discussionmentioning

confidence: 96%

Expression and distribution of S100 protein in the nervous system of the adult zebrafish (Danio rerio)

Germanà

Marino

Guerrera

et al. 2007

Microscopy Res & Technique

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S100 proteins are EF-hand calcium-binding protein highly preserved during evolution present in both neuronal and non-neuronal tissues of the higher vertebrates. Data about the expression of S100 protein in fishes are scarce, and no data are available on zebrafish, a common model used in biology to study development but also human diseases. In this study, we have investigated the expression of S100 protein in the central nervous system of adult zebrafish using PCR, Western blot, and immunohistochemistry. The central nervous system of the adult zebrafish express S100 protein mRNA, and contain a protein of approximately 10 kDa identified as S100 protein. S100 protein immunoreactivity was detected widespread distributed in the central nervous system, labeling the cytoplasm of both neuronal and non-neuronal cells. In fact, S100 protein immunoreactivity was primarily found in glial and ependymal cells, whereas the only neurons displaying S100 immunoreactivity were the Purkinje's neurons of the cerebellar cortex and those forming the deep cerebellar nuclei. Outside the central nervous system, S100 protein immunoreactivity was observed in a subpopulation of sensory and sympathetic neurons, and it was absent from the enteric nervous system. The functional role of S100 protein in both neurons and non-neuronal cells of the zebrafish central nervous system remains to be elucidated, but present results might serve as baseline for future experimental studies using this teleost as a model.

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“…The evidence for the existence of a robust expression of S100B in brain neurons is convincing for the mouse (Friend et al, 1992) and rat (Rickmann and Wolff, 1995a;Yang et al, 1995Yang et al, , 1996 yet, S100B is coined as a glialspecific marker of the CNS. Moreover, the consensus regarding the late ontogenic expression of S100B in the brain is at odds with several studies reporting the presence of S100B at early stages of prenatal development in several species, including humans (Landry et al, 1989(Landry et al, , 1990Sarnat, 1992;McKanna, 1993).…”

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confidence: 97%

Visualization of S100B‐positive neurons and glia in the central nervous system of EGFP transgenic mice

Vives

Alonso

Solal

et al. 2003

J of Comparative Neurology

148

130

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S100B, the EF-hand Ca(++)-binding protein with gliotrophic and neurotrophic properties implicated in the pathogenesis of Alzheimer's disease, is coined as a glial marker, despite its documented presence in rodent brain neurons. We have generated a transgenic mouse whose EGFP reporter, controlled by the -1,669/+3,106 sequence of the murine S100B gene, allows the direct microscopic observation of most S100B-expressing cells in the central nervous system (CNS). From embryonic day 13 onward, EGFP expression was targeted to selected neuroepithelial, glial, and neuronal cells, indicating that cell-specific expression of S100B is regulated at the transcriptional level during development. In adult mice, the highest level of EGFP expression was found in ependymocytes; astrocytes; and spinal, medullar, pontine, and deep cerebellar S100B neurons. Our results, thus, agree with earlier reports suggesting that S100B is not a CNS glial-specific marker. In addition, we detected EGFP and S100B in forebrain neurons previously thought not to express S100B in the mouse, including neurons of primary motor and somatosensory neocortical areas, the ventral pallidum and prerubral field. Another interesting finding was the selected EGFP targeting to neonatal S100B oligodendrocytes and adult NG2 progenitors as opposed to mature S100B oligodendrocytes. This finding suggests that, except for oligodendrocytes at the last stage of myelin maturation, the -1,669/+3,106 sequence of the S100B gene is a useful reagent for driving expression of transgenes in most S100B-expressing cells of mouse brain.

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S-100β has a neuronal localisation in the rat hindbrain revealed by an antigen retrieval method

Cited by 78 publications

References 54 publications

Differential expression of manganese and copper-zinc superoxide dismutases in the olfactory and vomeronasal receptor neurons of rats during ontogeny

Differential expression of manganese and copper-zinc superoxide dismutases in the olfactory and vomeronasal receptor neurons of rats during ontogeny

Expression and distribution of S100 protein in the nervous system of the adult zebrafish (Danio rerio)

Visualization of S100B‐positive neurons and glia in the central nervous system of EGFP transgenic mice

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