Saccharomyces cerevisiae Dmc1 and Rad51 Proteins Preferentially Function with Tid1 and Rad54 Proteins, Respectively, to Promote DNA Strand Invasion during Genetic Recombination

Nimonkar, Amitabh V.; Dombrowski, Christopher; Siino, Joseph S.; Stasiak, Alicja Z.; Stasiak, Andrzej; Kowalczykowski, Stephen C.

doi:10.1074/jbc.m112.373290

Cited by 57 publications

(83 citation statements)

References 60 publications

(57 reference statements)

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“…The meiotic roles of Rdh54 are consistent with its ability to interact with Dmc1 and to translocate on DNA to prevent the accumulation of Dmc1 on chromatin, thus allowing its intracellular recycling for meiotic recombination processes (14). Moreover, Rdh54 promotes Dmc1-mediated DNA strand invasion (22). The translocase activity of Rdh54 is also required to counteract Rad51 that accumulates on chromatin, either from overexpression of Rad51 or from loss of two other translocases, namely, Rad54 and Uls1 (16).…”

Section: Discussionmentioning

confidence: 70%

Characterization of the Interaction between the Saccharomyces cerevisiae Rad51 Recombinase and the DNA Translocase Rdh54

Maria

Kwon

Sung

et al. 2013

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 70%

Characterization of the Interaction between the Saccharomyces cerevisiae Rad51 Recombinase and the DNA Translocase Rdh54

Maria

Kwon

Sung

et al. 2013

Journal of Biological Chemistry

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“…However, the poor spore viability, which is in contrast to the wild-type-like spore viability of the poorly sporulating dmc1 strain, suggests that an AgKar3 function in karyogamy may be conserved in A. gossypii. Spo11 and Dmc1 are yeast meiotic proteins that catalyze the formation and subsequent repair of DSBs during meiotic recombination (Keeney 2001;Nimonkar et al 2012). Interestingly, deletion of AgSPO11 did not have an impact on sporulation in A. gossypii, whereas deletion of AgDMC1 severely reduced spore formation.…”

Section: Discussionmentioning

confidence: 99%

“…The sporulation levels, particularly in the dmc1 and kar3 mutants are reduced on solid media but are slightly improved in liquid media. In S. cerevisiae Dmc1 is required for repair of meiotic DSBs (Nimonkar et al 2012). Surprisingly, deletion of A. gossypii SPO11, which in S. cerevisiae initiates meiotic DSB formation (Keeney 2001), did not affect sporulation.…”

Section: Functional Analysis Of Sporulation Specific Genesmentioning

confidence: 99%

Molecular Determinants of Sporulation in Ashbya gossypii

et al. 2013

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Regulation of development and entry into sporulation is critical for fungi to ensure survival of unfavorable environmental conditions. Here we present an analysis of gene sets regulating sporulation in the homothallic ascomycete Ashbya gossypii. Deletion of components of the conserved pheromone/starvation MAP kinase cascades, e.g., STE11 and STE7, results in increased sporulation. In kar3 mutants sporulation is severely reduced, while deletion of KAR4 as well as of homologs of central Saccharomyces cerevisiae regulators of sporulation, IME1, IME2, IME4, and NDT80, abolishes sporulation in A. gossypii. Comparison of RNAseq transcript profiles of sporulation-deficient mutants identified a set of 67 down-regulated genes, most of which were up-regulated in the oversporulating ste12 mutant. One of these differentially expressed genes is an endoglucanase encoded by ENG2. We found that Eng2p promotes hyphal fragmentation as part of the developmental program of sporulation, which generates single-celled sporangia. Sporulationdeficient strains are arrested in their development but form sporangia. Supply of new nutrients enabled sporangia to return to hyphal growth, indicating that these cells are not locked in meiosis. Double-strand break (DSB) formation by Spo11 is apparently not required for sporulation; however, the absence of DMC1, which repairs DSBs in S. cerevisiae, results in very poor sporulation in A. gossypii. We present a comprehensive analysis of the gene repertoire governing sporulation in A. gossypii and suggest an altered regulation of IME1 expression compared to S. cerevisiae.A SHBYA gossypii is a riboflavin/vitamin B 2 overproducing filamentous ascomycete grouped within the Saccharomycetaceae (Kurtzman and Robnett 2003). A. gossypii belongs to the pre-whole genome duplication fungi but shares homologs of 95% of its genes with Saccharomyces cerevisiae (Deng et al. 2007). A. gossypii and its relative Eremothecium cymbalariae can complete their life cycle starting from a single spore that forms a sporulating mycelium and are thus homothallic (Wendland and Walther 2011). Sporulation in A. gossypii occurs at the end of its growth phase, when hyphae develop sporangia derived from septate compartments. Sporangia separate from each other by fragmentation of the hyphae at septal sites and spores are set free by lysis of the sporangia. A. gossypii spores are uninucleate and contain a haploid genome (Wendland and Walther 2005).In S. cerevisiae mating pheromones induce signaling via a conserved MAP kinase cascade that leads to the activation of the Ste12 transcription factor. Ste12 binds to the pheromone response element of target genes and induces their expression, which induces events leading to cell fusion and karyogamy (Bardwell 2005). In A. gossypii mating is apparently not required for sporulation as mutant strains deleted for both STE2 and STE3 pheromone receptor genes can still sporulate. On the contrary, deletion of the AgSTE12 homolog resulted in an oversporulation phenotype .Meiosis and sporulati...

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“…Most notably, Rad54 plays a more prominent role in the repair of certain types of DNA lesions and in the facilitation of repair synthesis, with Rdh54 apparently providing the major function in Rad51 removal from bulk chromatin (Ceballos and Heyer 2011). In both mitotic and meiotic cells, the promotion of HR between homologs is largely dependent on Rdh54, whereas Rad54 is more adept in the mediation of HR events between sister chromatids (Klein 1997;Shinohara et al 1997;Nimonkar et al 2012). Interestingly, the activity of the Rad51-Rad54 pair is down-regulated in meiosis via two different means, so as to permit interhomolog HR by the procrossover DSBR pathway.…”

Section: Promotion Of Dna Pairing and Repair Dna Synthesis By Rad54 Amentioning

confidence: 99%

Regulation of DNA Pairing in Homologous Recombination

Daley

Gaines

Kwon

et al. 2014

Cold Spring Harbor Perspectives in Biology

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Saccharomyces cerevisiae Dmc1 and Rad51 Proteins Preferentially Function with Tid1 and Rad54 Proteins, Respectively, to Promote DNA Strand Invasion during Genetic Recombination

Cited by 57 publications

References 60 publications

Characterization of the Interaction between the Saccharomyces cerevisiae Rad51 Recombinase and the DNA Translocase Rdh54

Characterization of the Interaction between the Saccharomyces cerevisiae Rad51 Recombinase and the DNA Translocase Rdh54

Molecular Determinants of Sporulation in Ashbya gossypii

Regulation of DNA Pairing in Homologous Recombination

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