Sacchathridine A, a Prostaglandin Release Inhibitor from Saccharothrix sp.

Abstract

Sacchathridine A (1) was isolated from the fermentation broth of strain Saccharothrix sp. MI559-46F5. The structure was determined as a new naphthoquinone derivative with an acetylhydrazino moiety by a combination of NMR, MS spectral analyses, and chemical degradation. Compound 1 showed inhibitory activity of prostaglandin E2 release in a concentration-dependent manner from human synovial sarcoma cells, SW982, with an IC50 value of 1.0 μM, but had no effect on cell growth up to 30 μM.

Non-streptomycete actinomycetes nourish the current microbial antibiotic drug discovery

“…According to a recent review by Tiwari and Gupta (2012), this non-streptomycete actinomycetes group comprising diverse bioactive secondary metabolite producing members under following genera: Actinomadura, Actinoplanes, Amycolatopsis, Dactylosporangium, Kibdelosporangium, Kitasatospora, Microbiospora, Planomonospora, Planobispora, Salinispora, Streptosporangium , and Verrucosispora . The list has further been extended by recent reports of bioactive compounds from members of other rare genera, Nonomuraea (Beltrametti et al, 2003; Jose and Jebakumar, 2012; Flatt et al, 2013), Actinoalloteichus (Fu et al, 2011; Wang et al, 2013), Pseudonocardia (Oh et al, 2009; Barke et al, 2010; Carr et al, 2012), Saccharothrix (Murakami et al, 2009; Aouiche et al, 2012; Nakae et al, 2013), and Actinosynnema (Wei et al, 2010; Siyu-Mao et al, 2012). Moreover, basic knowledge of the habitats, physiology, and secondary metabolite diversity of the rare actinomycetes gradually increased (Tiwari and Gupta, 2012).…”

Saccharothriolides A–C, novel phenyl-substituted 10-membered macrolides isolated from a rare actinomycete Saccharothrix sp.

“…1,2 Saccharothrix sp., one of the rare actinomycetes, was first obtained from a soil sample collected in Australia in 1984. 3 Dozens of bioactive natural products with a structural and biological diversity have been isolated from this genus: for example, a naphthoquinone derivative sacchathridine A which was reported as a prostaglandin release inhibitor, 4 16-membered macrolides tianchimycins A and B, 5 cytotoxic 20-membered macrolides ammocidins A-D, 6,7 antibacterial galacardins A and B, 8 and antitumor rebeccamycin. 9 In the course of our chemical screening for novel microbe metabolites, we discovered new 10-membered macrolides from a rare actinomycete Saccharothrix sp.…”

Structurally diverse metabolites from the rare actinobacterium Saccharothrix xinjiangensis

“…Determination of sugar moiety of caerulomycin A derivative was performed by hydrolysis according to Nakae et al 28…”