Safety evaluation of zinc methionine in laying hens: Effects on laying performance, clinical blood parameters, organ development, and histopathology

Chen, N N; Liu, Bing; Xiong, Pingwen; Guo, Yongzheng; He, Jinsong; Hou, Chunsheng; X, L; Yu, Dongmin

doi:10.3382/ps/pex400

Cited by 20 publications

(18 citation statements)

References 35 publications

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“…Significantly lower values of MCV and MCH ( Tables 6 and 7 ) were found only on D1 in group F3 in comparison with group C and group supplemented by Met (M), and this is difficult to explain. Whether alone or in combination with zinc, no effect of Met on hematological parameters in adult chickens was observed by Chen et al. (2018) .…”

Section: Discussionmentioning

confidence: 92%

The effect of methionine and folic acid administered in ovo on the hematological parameters of chickens (Gallus gallus domesticus)

et al. 2020

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Methionine ( Met ), an essential amino acid in poultry diets, when overdosed may cause hyperhomocysteinemia, which is mainly a trigger for cardiovascular diseases in humans. Homocysteine is neutralized (remethylated) in the presence of folic acid ( FA ), which also plays an important role in hematopoiesis and participates in the synthesis of DNA, and its deficiencies may result in the development of neural tube defects. One of the basic tools in studying the impact of both xenobiotics and nutrients on the animal organism is hematological analysis. Thus, the aim of this study was to determine the effect of in ovo supplementation with Met and FA on the hematological parameters of broiler chickens. On the 17th day of incubation, embryonated eggs (Ross 308) were injected with 5 or 25 mg of Met per egg (M5 and M25), 3 and 15 mg of FA per egg (F3 and F15), or a mixture of these 2 compounds (M5/F3 and M25/F15). The broilers were reared in accordance with welfare regulations and fed with commercial diets ad libitum . Blood samples were collected on the first, seventh, and 35th day of rearing (D1, D7, and D35), and complete hematological analysis was performed. The observed changes in red blood cell parameters probably result from physiological changes occurring during bird growth. Mean erythrocyte volume decreased with the age of chickens in the control, M5, and M25 groups, but not in those supplied with FA. Among supplemented groups, the number of white blood cells on D1 was lower only in group M5 than in the sham (C) group. The analysis of leukograms showed no significant differences between the groups. Comparing D1 with D7 in the group injected with a higher dose of Met and FA (MF25/15), a statistically significant increase in the percentage of lymphocytes and a significant decrease in the percentage of heterophils were observed. In addition, in the group injected with a higher FA dose (F15), there was statistically significant reduction in the percentage of eosinophils and a significant increase in the percentage of monocytes at day 7 compared with day 1. It seems that Met supplementation led to temporary immunosuppression in the animals.

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Section: Discussionmentioning

confidence: 92%

The effect of methionine and folic acid administered in ovo on the hematological parameters of chickens (Gallus gallus domesticus)

et al. 2020

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“…That is why the search for newer zinc antimicrobials still remains a big challenge. Interestingly, there are a few zinc proteinogenic amino acids complexes which are widely used in dietary supplements and animal feed, such as zinc bisglycinate and zinc methionine, but their use in dermatological products is rare [13][14][15]. It seems that conducting research in this direction could be very promising.…”

Section: Introductionmentioning

confidence: 99%

Zinc(II) Complexes with Amino Acids for Potential Use in Dermatology: Synthesis, Crystal Structures, and Antibacterial Activity

et al. 2020

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The multifunctional profile of Zn2+ has influenced its great popularity in various pharmaceutical, food, and cosmetic products. Despite the use of different inorganic and organic zinc derivatives, the search for new zinc-containing compounds with a safer skin profile still remains an open issue. The present paper describes the synthesis, structural characterization, and antibacterial activity of zinc(II) complexes with proteinogenic amino acids as potential candidates for dermatological treatments. The obtained complexes are of the general formula [Zn(AA)2], where AA represents an amino acid (L-Glu, Gly, L-His, L-Pro, L-Met, and L-Trp). Their synthesis was designed in such a way that the final bis(aminoacidate) zinc(II) complexes did not contain any counter-ions such as Cl−, NO3−, or SO42− that can cause some skin irritations. The chemical structure and composition of the compounds were identified by 1H NMR spectroscopy and elemental analysis, and four were also characterized by single-crystal X-ray diffraction. The Hirshfeld surface analysis for the Zn2+ metallic center helped to determine its coordination number and geometry for each complex. Finally, the antibacterial properties of the complexes were determined with respect to three Gram-positive strains, viz. Staphylococcus aureus ATCC 6538, Staphylococcus epidermidis ATCC 12228, and Streptococcus pyogenes ATCC 19615, and two Gram-negative bacteria, viz. Escherichia coli ATCC 25992 and Pseudomonas aeruginosa ATCC 27853, and were compared with the activity of zinc 2-pirrolidone 5-carboxylate (ZnPCA), commonly applied in dermatology. It was found that the Zn(II) complexes with methionine and glycine exhibited a higher antibacterial activity than the tested standard, and the antimicrobial properties of complex with Trp were satisfactory. The results of the antimicrobial activity examination allow us to postulate that the obtained zinc complexes might become new active substances for use in dermatological products.

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“…Дослідження критичної періодизації має важливе значення у ветеринарії та біології розмноження і розвитку (Kryshtoforova & Lemeshchenko, 2008;Vіshhur et al, 2010;Wójcik & Smalec, 2017). Дослідженню морфофункціональних показників крові в організмі качок в різні періоди постнатального онтогенезу присвячені роботи (Olayemi et al, 2002;Olubodun et al, 2015;Pavlova, 2015;Chen et al, 2018). Як зазначає Малюкін А.В.…”

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Stressor disorders of morphological indicators of bloods of ducks in critical periods of ontogenesis

Krogh

Stoyanovsky

2019

SMLNUVMB

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The article presents data hematol ohichnyh performance blood of ducks in critical periods of postnatal ontogenesis 2, 14, 21, 45, 90, 150, and 240 days of life, which are accordingly related to the full use of the yolk, intensive development of the digestive system and muscular tissue, change of fluff to the primary feather, juvenile molt, the beginning of the oviposition, the formation of the reproductive system and glands of the internal secretion, the continuation of the oviposition and the molt, which is accompanied by the complete renewal of the fine and medium feathers. Blood plays an important role in maintaining homeostasis , and studies of hematological parameters make it possible to assess the intensity of redox processes in tissues and organs, and studies of hematological parameters make it possible to estimate the intensity of redox processes in tissues and organs, namely the need for oxygen in connection with the physico-chemical and biochemical processes that occur in the body in the process of growth and development. They are dynamic and change with different factors faster than performance. The purpose of the study was to determine the dynamics of morphological parameters of duck blood during the critical periods of postnatal ontogeny . Research subject clinically healthy young Beijing duck meat breed performance direction. Changes in hematological parameters of ducks' blood reflect a picture of the impact of critical periods of ontogeny on ducks, characterized by 2 days of life, the numerical values of erythrocytes (2.45 ± 0.11 T/l) and the concentration of hemoglobin (102.90 ± 3.51 g/l), but the value of hematocrit was in the lower levels of physiological norms. While the leukocyte count of 31.20 ± 1.76 was close to the 240-day age, indicating high bird immunity after hatching , more likely to come from the use of yolk. In the study bird on the 14th day recorded an increase in the number of erythrocytes and leukocytes by 10.6 and 14.7%, hematocrit by 10.3% against a decrease in hemoglobin concentration by 12.8% (P < 0.05). At 21 days, the number of erythrocytes and hemoglobin concentration decreased by 8.2%, while hematocrit and leukocyte values approached the initial state of the experiment, amounting to 26.45 ± 2.40%, 32.06 ± 2.93 G/L. The presence of basophils in this age period indicates the launch of a chain of immune responses, indicating the formation of the immune system according to A. Malyukin. (2010). At day 45, erythrocytes and hemoglobin concentrations were reduced by 10.2% and 11.3% (P < 0.05), compared to the baseline period. During this period, hematocrit increased by 12.7%, which indicated an increase in blood cells in the ducts. Results at 90 days indicated a decrease in erythrocyte count by 14.3% (P < 0.05) and hemoglobin concentration by 11.8% (P < 0.05), while leukocyte count and hematocrit increased by 26.1 and 22.1% (P < 0.05) compared with ducklings of 2 days of age. In ducklings at 150 days of age, the erythrocyte count and hemoglobin concentration were close to the baseline, and the leukocyte count and hematocrit were increased by 19.6 and 39.3%, respectively (P < 0.05). At 240 days of ducklings life, an increase in the oxygen-transport system of blood was established in the form of an increase in the number of erythrocytes by 35.1% (P < 0.01), hemoglobin concentration by 28.7% (P < 0.01), hematocrit value by 50, 8% (P < 0.01) compared to 2-days of ducklings life, and the number of leukocytes was close to baseline, indicating a decrease in the reactivity of the organism.

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Safety evaluation of zinc methionine in laying hens: Effects on laying performance, clinical blood parameters, organ development, and histopathology

Cited by 20 publications

References 35 publications

The effect of methionine and folic acid administered in ovo on the hematological parameters of chickens (Gallus gallus domesticus)

The effect of methionine and folic acid administered in ovo on the hematological parameters of chickens (Gallus gallus domesticus)

Zinc(II) Complexes with Amino Acids for Potential Use in Dermatology: Synthesis, Crystal Structures, and Antibacterial Activity

Stressor disorders of morphological indicators of bloods of ducks in critical periods of ontogenesis

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