Saliterribacillus persicus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a hypersaline lake

Abstract

Saliterribacillus persicus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a hypersaline lake , was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain X4BT were motile rods and formed ellipsoidal endospores at a terminal or subterminal position in swollen sporangia. Strain X4B T was a strictly aerobic bacterium, catalase-and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-22.5 % (w/v), with optimum growth occurring at 7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 6C and pH 7.0. Analysis of 16S rRNA gene sequence revealed that strain X4B T is a member of the family Bacillaceae, constituting a novel phyletic lineage within this family. Highest sequence similarities were obtained with the 16S rRNA gene sequences of the type strains of Sediminibacillus albus (96.0 %), Paraliobacillus ryukyuensis (95.9 %), Paraliobacillus quinghaiensis (95.8 %) and Sediminibacillus halophilus (95.7 %), respectively. The DNA G+C content of this novel isolate was 35.2 mol%. The major cellular fatty acids of strain X4B T were anteiso-C 15 : 0 and anteiso-C 17 : 0 and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two aminolipids, an aminophospholipid and an unknown phospholipid. The isoprenoid quinones were MK-7 (89 %) and MK-6 (11 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain X4B T represents a novel species in a new genus in the family Bacillaceae, order Bacillales for which the name Saliterribacillus persicus gen. nov., sp. nov. is proposed. The type strain of the type species (Saliterribacillus persicus) is X4B

Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.

“…The family Bacillaceae is one of the largest taxa in the order Bacillales in which several novel genera have been reported. Some of these recently described genera include Streptohalobacillus (Wang et al, 2011), Allobacillus (Sheu et al, 2011), Natribacillus (Echigo et al, 2012), Domibacillus (Seiler et al, 2013), Saliterribacillus (Amoozegar et al, 2013), Fictibacillus (Glaeser et al, 2013), Sinibacillus (Yang & Zhou, 2014), Thermolongibacillus (Cihan et al, 2014), Aquibacillus (Amoozegar et al, 2014), Aureibacillus , Compostibacillus , Melghiribacillus (Addou et al, 2015) and Salibacterium (Reddy et al, 2015). In this study, we propose a novel species of a new genus in the family Bacillaceae, by characterizing two strains (S9 T and S12) based on the minimal standards (Logan et al, 2009) proposed for the description of new taxa of aerobic, endospore-forming bacteria.…”

Melghiribacillus thermohalophilus gen. nov., sp. nov., a novel filamentous, endospore-forming, thermophilic and halophilic bacterium

“…Halophilic bacteria are classified as slightly halophilic, moderately halophilic or extreme halophilic, according to their salt requirements (Kushner, 1985). They are represented by heterogeneous physiological and taxonomic groups (Ventosa et al , 1998a; Ventosa, 2006; Sanchez-Porro et al , 2009; Amoozegar et al , 2013), and are largely found within aerobic or facultatively anaerobic, Gram-staining-positive, endospore-forming bacteria. The term ‘aerobic endospore-forming bacteria’ is used to embrace species of Bacillus and related genera, and the production of resistant endospores in the presence of oxygen has long been their defining feature (Logan & Halket, 2011).…”

Salinibacillus xinjiangensis sp. nov., a halophilic bacterium from a hypersaline lake

“…Many new genera of Gram-positive, moderately halophilic bacteria (MHB), which grow well in media with 3 to 15 % (w/v) salt (de la Haba et al , 2011; Ren & Zhou (2005b)), have been reported within this family. At the time of writing, the known MHB genera of the family Bacillaceae contained Saliterribacillus (Amoozegar et al , 2013), Alteribacillus (Didari et al , 2012), Salisediminibacterium (Jiang et al , 2012), Natribacillus (Echigo et al 2012), Streptohalobacillus (Wang et al , 2011), Sediminibacillus (Carrasco et al , 2008), Aquisalibacillus (Márquez et al , 2008), Salimicrobium (Yoon et al , 2007), Salsuginibacillus (Carrasco et al , 2007), Piscibacillus (Tanasupawat et al , 2007), Pelagibacillus (Kim et al , 2007), Salinibacillus (Ren & Zhou, 2005b), Tenuibacillus (Ren & Zhou, 2005a), Thalassobacillus (García et al , 2005), Pontibacillus (Lim et al , 2005), Alkalibacillus (Jeon et al , 2005), Halobacillus (Spring et al , 1996), Virgibacillus (Heyndrickx et al , 1998), Gracilibacillus (Wainø et al , 1999), Filobacillus (Schlesner et al , 2001), Oceanobacillus (Lu et al , 2001) and ...…”