Salt and cadmium stress tolerance caused by overexpression of the Glycine Max Na+/H+ Antiporter (GmNHX1) gene in duckweed (Lemna turionifera 5511)

Yang, Lin; Han, Yahong; Wu, Di; Wang, Yong; Liu, Miaomiao; Wang, Sutong; Liu, Wenxin; Lu, Mei-Yi; Wei, Ying; Sun, Jiufeng

doi:10.1016/j.aquatox.2017.08.010

Cited by 42 publications

(20 citation statements)

References 36 publications

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“…For example, GmSALT3/GmCHX1 ( Glyma.03G171600 ) which is a gene associated with salt tolerance with great potential for soybean improvement showed down-regulated expression pattern after salt treatment (Guan et al, 2014; Qi et al, 2014; Liu et al, 2016). The Na +/H+ antiporter gene GmNHX1 ( Glyma.20G229900 ) which can enhance salt tolerance of soybean roots (Li et al, 2006; Sun et al, 2006; Yang et al, 2017). A soybean glycogen synthase kinase 3 gene which can enhance tolerance to salt was up-regulated in salt treated soybean in this study (Wang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

et al. 2019

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Soybean is an important economic crop for human diet, animal feeds and biodiesel due to high protein and oil content. Its productivity is significantly hampered by salt stress, which impairs plant growth and development by affecting gene expression, in part, through epigenetic modification of chromatin status. However, little is known about epigenetic regulation of stress response in soybean roots. Here, we used RNA-seq and ChIP-seq technologies to study the dynamics of genome-wide transcription and histone methylation patterns in soybean roots under salt stress. Eight thousand seven hundred ninety eight soybean genes changed their expression under salt stress treatment. Whole-genome ChIP-seq study of an epigenetic repressive mark, histone H3 lysine 27 trimethylation (H3K27me3), revealed the changes in H3K27me3 deposition during the response to salt stress. Unexpectedly, we found that most of the inactivation of genes under salt stress is strongly correlated with the de novo establishment of H3K27me3 in various parts of the promoter or coding regions where there is no H3K27me3 in control plants. In addition, the soybean histone modifiers were identified which may contribute to de novo histone methylation and gene silencing under salt stress. Thus, dynamic chromatin regulation, switch between active and inactive modes, occur at target loci in order to respond to salt stress in soybean. Our analysis demonstrates histone methylation modifications are correlated with the activation or inactivation of salt-inducible genes in soybean roots.

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Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

et al. 2019

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“…In comparison to previous Agrobacterium ‐mediated transformation protocols in L. minor (Cantó‐Pastor et al ., ; Chhabra et al ., ; Firsov et al ., , ; Ko et al ., ; Sun et al ., ; Yamamoto et al ., ; Yang et al ., , , ), the increased efficiency of our protocol is likely resulted from adopting sonication coupled with vacuum infiltration infection method. In several previous studies, such as banana (Subramanyam et al ., ), chickpea (Indurker et al ., ) and cowpea (Bakshi et al ., ), it has been shown that the combination of vacuum infiltration and sonication can greatly improve the efficiency of Agrobacterium infection.…”

Section: Discussionmentioning

confidence: 99%

“…The development of genetic manipulation tools requires both an efficient transformation protocol and an effective genome editing system. Among the 37 duckweed species that belong to five genera: Spirodela , Landoltia , Lemna , Wolffiella and Wolffia (Landolt, ; Sree et al ., ), the Agrobacterium‐ mediated transformation system has been developed in at least seven of them, including Lemna gibba (Cantó‐Pastor et al ., ; Yamamoto et al ., ), Lemna minor (Chhabra et al ., ; Firsov et al ., , ; Ko et al ., ; Sun et al ., ; Yamamoto et al ., ; Yang et al ., , ), Lemna turionifera (Yang et al ., ), Landoltia punctata (formerly Spirodela oligorrhiza ) (Rival et al ., ; Vunsh et al .,), Spirodela polyrhiza (Yang et al ., ), Wolffia arrhiza (Khvatkov et al ., , ) and Wolffia globosa (Heenatigala et al ., ). However, the efficiency of the transformation varied dramatically among species (from 0.14% to 82.5%).…”

Section: Introductionmentioning

confidence: 99%

Efficient genetic transformation and CRISPR/Cas9‐mediated genome editing in Lemna aequinoctialis

Liu

Wang

et al. 2019

Plant Biotechnology Journal

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Summary The fast growth, ease of metabolic labelling and potential for feedstock and biofuels production make duckweeds not only an attractive model system for understanding plant biology, but also a potential future crop. However, current duckweed research is constrained by the lack of efficient genetic manipulation tools. Here, we report a case study on genome editing in a duckweed species, Lemna aequinoctialis, using a fast and efficient transformation and CRISPR/Cas9 tool. By optimizing currently available transformation protocols, we reduced the duration time of Agrobacterium‐mediated transformation to 5–6 weeks with a success rate of over 94%. Based on the optimized transformation protocol, we generated 15 (14.3% success rate) biallelic LaPDS mutants that showed albino phenotype using a CRISPR/Cas9 system. Investigations on CRISPR/Cas9‐mediated mutation spectrum among mutated L. aequinoctialis showed that most of mutations were short insertions and deletions. This study presents the first example of CRISPR/Cas9‐mediated genome editing in duckweeds, which will open new research avenues in using duckweeds for both basic and applied research.

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“…For instance, AtCHX17 mutant accumulated less K + than wide type under the salt stress and K + deficiency environment, indicating that AtCHX17 was involved in the absorption and transport of K + [28]. Among the CPA genes in other plants, it was NHX1 that could decrease the salt-tolerance ability of kallar grass at the concentration of 100 and 150 µM cadmium concentrations [43]. Moreover, NHX1 regulated Cd 2+ and H + flow during short-term Cd 2+ shock and confirmed that it could enhance tolerance during Cd 2+ stress [44].…”

Section: Roles Of Rscpa Genes In Response To Different Abiotic Stressesmentioning

confidence: 99%

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

Wang

Ying

Yang

et al. 2020

IJMS

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The CPA (cation proton antiporter) family plays an essential role during plant stress tolerance by regulating ionic and pH homeostasis of the cell. Radish fleshy roots are susceptible to abiotic stress during growth and development, especially salt stress. To date, CPA family genes have not yet been identified in radish and the biological functions remain unclear. In this study, 60 CPA candidate genes in radish were identified on the whole genome level, which were divided into three subfamilies including the Na+/H+ exchanger (NHX), K+ efflux antiporter (KEA), and cation/H+ exchanger (CHX) families. In total, 58 of the 60 RsCPA genes were localized to the nine chromosomes. RNA-seq. data showed that 60 RsCPA genes had various expression levels in the leaves, roots, cortex, cambium, and xylem at different development stages, as well as under different abiotic stresses. RT–qPCR analysis indicated that all nine RsNHXs genes showed up regulated trends after 250 mM NaCl exposure at 3, 6, 12, and 24h. The RsCPA31 (RsNHX1) gene, which might be the most important members of the RsNHX subfamily, exhibited obvious increased expression levels during 24h salt stress treatment. Heterologous over-and inhibited-expression of RsNHX1 in Arabidopsis showed that RsNHX1 had a positive function in salt tolerance. Furthermore, a turnip yellow mosaic virus (TYMV)-induced gene silence (VIGS) system was firstly used to functionally characterize the candidate gene in radish, which showed that plant with the silence of endogenous RsNHX1 was more susceptible to the salt stress. According to our results we provide insights into the complexity of the RsCPA gene family and a valuable resource to explore the potential functions of RsCPA genes in radish.

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Salt and cadmium stress tolerance caused by overexpression of the Glycine Max Na+/H+ Antiporter (GmNHX1) gene in duckweed (Lemna turionifera 5511)

Cited by 42 publications

References 36 publications

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

Efficient genetic transformation and CRISPR/Cas9‐mediated genome editing in Lemna aequinoctialis

Genome-Wide Identification and Functional Characterization of the Cation Proton Antiporter (CPA) Family Related to Salt Stress Response in Radish (Raphanus sativus L.)

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