Salvia miltiorrhiza polysaccharides protect against lipopolysaccharide-induced liver injury by regulating NF-κb and Nrf2 pathway in mice

Abstract

2019) Salvia miltiorrhiza polysaccharides protect against lipopolysaccharide-induced liver injury by regulating NF-κb and Nrf2 pathway in mice, Food and Agricultural Immunology, 30:1, 979-994, ABSTRACTThe current study was conducted to investigate the hepatoprotective effects of Salvia miltiorrhiza polysaccharides (SMP) against lipopolysaccharide (LPS) induced liver injury. The mice were treated with SMP first and LPS later. Results showed that SMPs significantly reduced the activities of serum alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin (TBIL), malondialdehyde (MDA) and contents of inflammation factors and mRNA expressions in LPS induced liver injury mice. However, SMP significantly increased the contents of glutathione (GSH), Superoxide dismutase (SOD) and Total antioxidant capacity (T-AOC). SMP also downregulated the expressions of p-p65, p-IκBα, inducible nitricoxide synthase (iNOS) and improved the expression of Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and Heme Oxygenase-1 (HO-1). The study indicates SMP protects the liver via attenuating inflammatory reactions and antioxidant effects.

L-Arginine protects ovine intestinal epithelial cells from lipopolysaccharide-induced intestinal barrier injury

“…Lipopolysaccharide (LPS) is the principal outer membrane component in Gram-negative bacteria, which can induce elevated oxidative injury to IECs through generating various reactive oxygen species (ROS), and the latter can lead to lipid peroxidation and apoptosis (Han et al, 2019;Talavera et al, 2017). In this study, LPS was employed to construct a cell injury model to study the protective mechanism of Arg in the intestinal epithelial barrier function of ovine intestinal epithelial cells (IOECs).…”

Anti-tumor activity of polysaccharides obtained from Leucocalocybe mongolica using solid-state fermentation

“…Immunohistochemical analysis was performed according to a previously described method [12]. After restoration in citric acid buffer (0.01 mol/L, pH 6.0), 1% H 2 O 2 solution was added to the slides to block peroxidase activity.…”

“…As one of the main bioactive components, polysaccharides are a key research topic [11,12]. Polysaccharides are carbohydrate polymers composed of monosaccharide units bound together by glycosidic linkages, and they are involved in a range of biological processes [13].…”

Protective role of Salvia miltiorrhiza Polysaccharides on Florfenicol Induced Oxidative and Apoptotic Injury of Liver in Broilers

“…Modern studies have found that SMPs also has the protective effect of liver injury (Song et al 2008;Sun et al 2011). Our previous research found that SMPs can inhibit in ammatory pathways TRL4/MyD88, NF-κB and chemokine CXCL-10 to reduce the release of in ammatory factors, so as to inhibit the in ammatory response, and then alleviate immune liver injury (Han et al 2019b;Han et al 2018;Wang et al 2019). In addition, SMPs can effectively regulate oxidative stress in the liver of mice, inhibit apoptosis of hepatocyte, and then alleviate liver injury (Han et al 2019b;Wang et al 2019).…”

“…Our previous research found that SMPs can inhibit in ammatory pathways TRL4/MyD88, NF-κB and chemokine CXCL-10 to reduce the release of in ammatory factors, so as to inhibit the in ammatory response, and then alleviate immune liver injury (Han et al 2019b;Han et al 2018;Wang et al 2019). In addition, SMPs can effectively regulate oxidative stress in the liver of mice, inhibit apoptosis of hepatocyte, and then alleviate liver injury (Han et al 2019b;Wang et al 2019). At the same time, we also found that SMPs can improve liver drug enzyme activity in liver, reduce oxidative stress of liver cells, so that, SMPs also had a good alleviation effect on chicken liver injury caused by CCL 4 (Han et al 2019a).…”